A5089865758- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Wastewater Treatment and Nitrogen Removal
- Metal Extraction and Bioleaching
- Microbial Fuel Cells and Bioremediation
- Protist diversity and phylogeny
- Hydrocarbon exploration and reservoir analysis
- Environmental DNA in Biodiversity Studies
- Radioactive contamination and transfer
- Bacteriophages and microbial interactions
- Anaerobic Digestion and Biogas Production
- Photosynthetic Processes and Mechanisms
- Mine drainage and remediation techniques
- Radioactive element chemistry and processing
- Metabolomics and Mass Spectrometry Studies
- Enzyme function and inhibition
- Microbial Applications in Construction Materials
- Calcium Carbonate Crystallization and Inhibition
- Mitochondrial Function and Pathology
- Geological Studies and Exploration
- Oral microbiology and periodontitis research
- Coal Properties and Utilization
- CO2 Sequestration and Geologic Interactions
- Atmospheric and Environmental Gas Dynamics
Russian Academy of Sciences
2012-2024
Yandex (Russia)
2024
Czech Academy of Sciences, Institute of Microbiology
2003-2024
Federal Center Research Fundamentals of Biotechnology
2021-2024
Lomonosov Moscow State University
2011
Engelhardt Institute of Molecular Biology
2001
ABSTRACT Activity measurements by radioisotopic methods and cultural molecular approaches were used in parallel to investigate the microbial biodiversity its physiological potential formation waters of Samotlor high-temperature oil reservoir (Western Siberia, Russia). Sulfate reduction with rates not exceeding 20 nmol H 2 S liter −1 day occurred at 60 80°C. In upper horizons (AB, A, B), methanogenesis (lithotrophic and/or acetoclastic) was detected only wells which sulfate did occur. some...
A novel, moderately thermophilic, strictly anaerobic, mixotrophic bacterium, designated strain LF13T, was isolated from a deep-sea hydrothermal chimney sample that collected at vent site 14° 45′ N, 44° 59′ W on the Mid-Atlantic Ridge. Cells were Gram-negative, thin, non-motile rods of variable length. Strain LF13T grew optimally pH 6·8–7·0 and 60 °C with 2·5 % (w/v) NaCl. It chemo-organoheterotrophically, fermenting proteinaceous substrates, pyruvate Casamino acids. The able to grow by...
ABSTRACT Three new molecular approaches were developed to identify drug-resistant strains of Mycobacterium tuberculosis using biochips with oligonucleotides immobilized in polyacrylamide gel pads. These are significantly faster than traditional bacteriological methods. All three approaches—hybridization, PCR, and ligase detection reaction—were designed analyze an 81-bp fragment the gene rpoB encoding β-subunit RNA polymerase, where most known mutations rifampin resistance located. The call...
A thermophilic, anaerobic, spore-forming, dissimilatory Fe(III)-reducing bacterium, designated strain SR4T, was isolated from sediment of newly formed hydrothermal vents in the area eruption Karymsky volcano on Kamchatka peninsula. Cells SR4T were straight-to-curved, peritrichous rods, 0.4-0.6 μ diameter and 3.5-9.0 length, exhibited a slight tumbling motility. Strain round, refractile, heatresistant endospores terminally swollen sporangia. The temperature range for growth 39–78 °C with an...
A new anaerobic, thermophilic, facultatively carboxydotrophic bacterium, strain Nor1 T , was isolated from a hot spring at Norris Basin, Yellowstone National Park. Cells of were curved motile rods with length 2·6–3 μm, width about 0·5 μm and lateral flagellation. The cell wall structure the Gram-negative type. Strain thermophilic (temperature range for growth 40–68 °C, an optimum 60 °C) neutrophilic (pH 6·5–7·6, 6·8–7·0). It grew chemolithotrophically on CO (generation time, 1·15 h),...
The genome of Caldithrix abyssi, the first cultivated representative a phylum-level bacterial lineage, was sequenced within framework Genomic Encyclopedia Bacteria and Archaea (GEBA) project. genomic analysis revealed mechanisms allowing this anaerobic bacterium to ferment peptides or implement nitrate reduction with acetate molecular hydrogen as electron donors. encoded five different [NiFe]- [FeFe]-hydrogenases, one which, group 1 [NiFe]-hydrogenase, is presumably involved in...
Respiratory ammonification of nitrate is the microbial process that determines retention nitrogen in an ecosystem. To date, sulfur-dependent dissimilatory reduction to ammonium has been demonstrated only with sulfide as electron donor. We detected a novel pathway couples sulfur and cycles. Thermophilic anaerobic bacteria Thermosulfurimonas dismutans Dissulfuribacter thermophilus, isolated from deep-sea hydrothermal vents, grew autotrophically elemental donor acceptor producing sulfate...
The Calvin–Benson–Bassham (CBB) cycle assimilates CO 2 for the primary production of organic matter in all plants and algae, as well some autotrophic bacteria. key enzyme CBB cycle, ribulose-bisphosphate carboxylase/oxygenase (RubisCO), is a main determinant de novo on Earth. Of three carboxylating forms RubisCO, I II participate autotrophy, form III so far has been associated only with nucleotide nucleoside metabolism. Here, we report that RubisCO functions thermophilic...
A new anaerobic, thermophilic, CO-utilizing marine bacterium, strain JMT, was isolated from a submarine hot vent in Okinawa Trough. Cells of JMT were non-motile thin straight rods, sometimes branching, with cell wall the Gram-positive type, surrounded an S-layer. Chains three to five cells often observed. The isolate grew chemolithotrophically on CO, producing equimolar quantities H2 and CO2 (according equation CO+H2O-->CO2+H2) organotrophically peptone, yeast extract, starch, cellobiose,...
A novel anaerobic, thermophilic, CO-utilizing bacterium, strain 41(T), was isolated from a terrestrial hot vent on the Kamchatka Peninsula. Strain 41(T) found to be Gram-positive its cells being short, straight, motile rods. Chains of three five were often observed. The isolate grew only chemolithoautotrophically CO, producing equimolar quantities H2 and CO2 (according equation CO+H2O --> CO2+H2). Growth observed in temperature range 40-68 degrees C, with an optimum at 58 pH 6.5-7.6, 7.0....
A novel anaerobic, thermophilic, alkalitolerant bacterium, strain 2204 T , was isolated from a hot spring of the Baikal Lake region. The cells were straight rods variable length, Gram-positive with an S-layer, motile one to two lateral flagella, and often formed aggregates 3–15 cells. isolate shown be obligate anaerobe oxidizing CO producing equimolar quantities H 2 according equation CO+H O→CO +H . No organic substrates used as energy sources. For lithotrophic growth on CO, 0·2 g acetate or...
An obligately anaerobic, sulfate-reducing micro-organism, strain 3127-1T, was isolated from geothermally heated soil (Oil Site, Uzon Caldera, Kamchatka, Russia). The new isolate a moderately thermoacidophilic anaerobe able to grow with H2 or formate by respiration of sulfate thiosulfate. pH range for growth 3.7-6.5, an optimum at 4.8-5.0. temperature 37-65 °C, 55 °C. G+C content the genomic DNA 33.7 mol%. genome 3127-1T contained two almost identical 16S rRNA genes, differing single...
The goal of this work was to study the diversity microorganisms inhabiting a deep subsurface aquifer system in order understand their functional roles and interspecies relations formed course buried organic matter degradation. A microbial community thermal Tomsk Region, Western Siberia monitored over five years via 2.7 km borehole 3P, drilled down Palaeozoic basement. water discharges with temperature ca. 50oC. Its chemical composition varies, but it steadily contains acetate, propionate,...
Terrestrial mud volcanoes (TMVs) are important natural sources of methane emission. The microorganisms inhabiting these environments remain largely unknown. We studied the phylogenetic composition and metabolic potential prokaryotic communities TMVs located in Taman Peninsula, Russia, using a metagenomic approach. One examined sites harbored unique community with high abundance anaerobic methane-oxidizing archaea belonging to ANME-3 group (39% all 16S rRNA gene reads). number was confirmed...
The key microbial group involved in anaerobic methane oxidation is methanotrophic archaea (ANME). From a terrestrial mud volcano, we enriched community containing ANME-2a, using as an electron donor, Fe(III) oxide (ferrihydrite) acceptor, and anthraquinone-2,6-disulfonate shuttle. Ferrihydrite reduction led to the formation of black, highly magnetic precipitate. A significant relative abundance ANME-2a batch cultures was observed over five subsequent transfers. Phylogenetic analysis revealed...
New thermoacidophilic organisms that were able to grow anaerobically on starch isolated from the acidic hot springs of Kamchatka. Strain 1904T, a spring Moutnovski volcano, was characterized in detail. Its cells regular or irregular cocci 1-2 microm diameter, non-motile, and had cell envelope consisting one layer subunits. The new organism hyperthermophile, growing temperature range 60-92 degrees C (with an optimum at 85 C), acidophile, having pH for growth 2.0-6.0 3.8), obligate anaerobe....
Two extremely thermophilic archaea, designated W-12 and P-4, were isolated from a geothermal vent in the tidal zone of Whale Island, New Zealand, geothermally heated bottom deposits Bay Plenty, respectively. Cells isolate are irregular cocci, 0.3-1.2 microns diameter, motile with polar flagella. The cell envelope consists one layer subunits major protein M(r) 75,000. produce protrusions different kinds: prostheca-like, chains bubbles, or network fimbriae. P-4 regular 0.7-1.0 micron two...
An obligately anaerobic, hyperthermophilic, organoheterotrophic archaeon, strain Z-1312(T), was isolated from a freshwater hot spring of the Uzon caldera (Kamchatka Peninsula, Russia). The cells were regular cocci, 1-4 microm in diameter, with one long flagellum. cell envelope composed globular layer attached to cytoplasmic membrane. temperature range for growth 63-89 degrees C, an optimum between 80 and 82 C. pH at C 4.8-6.8, 6.0. Strain Z-1312(T) grew by hydrolysis and/or fermentation wide...
An extremely thermophilic, strictly anaerobic, facultatively chemolithoautotrophic bacterium designated strain DST was isolated from Treshchinnyi Spring, one of the hottest springs Uzon Caldera (Kamchatka, Russia). Cells novel organism were Gram-negative rods, about 1.0–1.2 μm long and 0.5 wide. The temperature range for growth 52–82 °C, with an optimum at 75 °C. Growth observed pH 6.8–7.4, 7.0–7.2. Strain able to grow lithoautotrophically hydrogen in presence CO2 as a carbon source...
A thermophilic, anaerobic, chemolithoautotrophic bacterium (designated strain SL50T) was isolated from a hydrothermal sample collected at the Mid-Atlantic Ridge deepest of known World ocean fields, Ashadze field (1 ° 58′ 21″ N 4 51′ 47″ W) depth 4100 m. Cells SL50T were motile, straight to bent rods with one polar flagellum, 0.5–0.6 μm in width and 3.0–3.5 length. The temperature range for growth 25–75 °C, an optimum 60 °C. pH 5.0–7.5, 6.5. Growth observed NaCl concentrations ranging 1.0 6.0...