A5045129875- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Insect symbiosis and bacterial influences
- Enzyme Production and Characterization
- Biocrusts and Microbial Ecology
- Tardigrade Biology and Ecology
- Parasite Biology and Host Interactions
- Marine Bivalve and Aquaculture Studies
- Genomics and Phylogenetic Studies
- Plant Parasitism and Resistance
- Microbial Metabolites in Food Biotechnology
- Spaceflight effects on biology
- Biofuel production and bioconversion
- Insect and Pesticide Research
- Microfluidic and Capillary Electrophoresis Applications
- Marine Biology and Ecology Research
- Microfluidic and Bio-sensing Technologies
- Aquatic Invertebrate Ecology and Behavior
- Dengue and Mosquito Control Research
- Microbial Metabolism and Applications
- Enzyme Structure and Function
- Heat Transfer and Boiling Studies
- Electrowetting and Microfluidic Technologies
- Gut microbiota and health
- Bacteriophages and microbial interactions
Tokyo Institute of Technology
2003-2023
The University of Tokyo
2011-2016
Japan Agency for Marine-Earth Science and Technology
2007-2011
Osaka University
2011
Kagoshima Prefectural College
1997
Tardigrades, also known as water bears, are small aquatic animals. Some tardigrade species tolerate almost complete dehydration and exhibit extraordinary tolerance to various physical extremes in the dehydrated state. Here we determine a high-quality genome sequence of Ramazzottius varieornatus, one most stress-tolerant species. Precise gene repertoire analyses reveal presence proportion (1.2% or less) putative foreign genes, loss pathways that promote stress damage, expansion families...
Tardigrades are able to tolerate almost complete dehydration by reversibly switching an ametabolic state. This ability is called anhydrobiosis. In the anhydrobiotic state, tardigrades can withstand various extreme environments including space, but their molecular basis remains largely unknown. Late embryogenesis abundant (LEA) proteins heat-soluble and prevent protein-aggregation in dehydrated conditions other organisms, relevance tardigrade anhydrobiosis not clarified. this study, we...
Wood-feeding lower termites harbour symbiotic gut protists that support the termite nutritionally by degrading recalcitrant lignocellulose. These themselves host specific endo- and ectosymbiotic bacteria, functions of which remain largely unknown. Here, we present draft genomes a dominant, uncultured ectosymbiont belonging to order Bacteroidales, 'Candidatus Symbiothrix dinenymphae', colonizes cell surface cellulolytic Dinenympha spp. We analysed four single-cell Ca. S. dinenymphae, highest...
Termite guts harbor diverse yet-uncultured bacteria, including a non-photosynthetic cyanobacterial group, the class "Melainabacteria". We herein reported phylogenetic diversity of "Melainabacteria" in termites and conducted single-cell genome analysis melainabacterium obtained from gut termite Termes propinquus. performed amplicon sequencing 16S rRNA genes 60 eight cockroach species, detected melainabacterial sequences 48 out 68 insect albeit with low abundances (0.02-1.90%). Most were...
Abstract The cellulolytic protist Trichonympha agilis in the termite gut permanently hosts two symbiotic bacteria, ‘Candidatus Endomicrobium trichonymphae’ and Desulfovibrio trichonymphae’. former is an intracellular symbiont, latter almost but still connected to outside via a small pore. complete genome of ‘Ca. has previously been reported, we here present (1 410 056 bp), many pseudogenes, retains biosynthetic pathways for various amino acids cofactors, which are partially complementary...
Tardigrades are tiny (less than 1 mm in length) invertebrate animals that have the potential to survive travel other planets because of their tolerance extreme environmental conditions by means a dry ametabolic state called anhydrobiosis. While adult tardigrades environments has been reported, there few reports on eggs. We examined ability hydrated and anhydrobiotic eggs tardigrade Ramazzottius varieornatus hatch after exposure ionizing irradiation (helium ions), extremely low high...
Termites depend nutritionally on their gut microbes, and protistan, bacterial, archaeal communities have been extensively studied. However, limited information is available viruses in the termite gut. We herein report complete genome sequence (99,517 bp) of a phage obtained during analysis "Candidatus Azobacteroides pseudotrichonymphae" phylotype ProJPt-1, which an obligate intracellular symbiont cellulolytic protist Pseudotrichonympha sp. Prorhinotermes japonicus. The phage, designated...
A bacterium that secreted cyclodextrin glucanotransferase (CGTase) in a medium overlaid with n-hexane was isolated and identified as Paenibacillus illinoisensis strain ST-12 K. The CGTase of the purified from culture supernatant. molecular mass 70 kDa. enzyme stable at pH 6 to 10 active 5.0 8.0. optimum temperature 7.0 65 degrees C presence 5 mM CaCl2. produced mainly beta-cyclodextrin. total yield alpha-, beta-, gamma- cyclodextrins increased 1.4-fold by addition ethanol. In particular,...
Abstract Background Two Calyptogena clam intracellular obligate symbionts, Ca . Vesicomyosocius okutanii (Vok; C. symbiont) and Ruthia magnifica (Rma; symbiont), have small genomes (1.02 1.16 Mb, respectively) with low G+C contents (31.6% 34.0%, are thought to be in an ongoing stage of reductive genome evolution (RGE). They lack recA some genes for DNA repair, including mutY The loss is contribute the stabilization their architectures GC bias, respectively. To understand how these were lost...
Abstract Several Trichonympha protist species in the termite gut have independently acquired Desulfovibrio ectosymbionts apparently different stages of symbiosis. Here, we obtained near-complete genome sequence phylotype ZnDsv-02, which attaches to surface collaris cells, and compared it with a previously ‘Candidatus trichonymphae’ Rs-N31, is almost completely embedded cytoplasm agilis. Single-nucleotide polymorphism analysis indicated that although Rs-N31 clonal, ZnDsv-02 population on...
Outlines the fundamental considerations for a planetary rover. First, shapes which utilize limitless rotation such as wheels and crawlers, legged shapes, articulated body were compared, it was found that wheel type is currently optimum Next, authors study specific methods configuring rovers with one, two, three wheels, topic has not been studied much before, in particular, indicate effective to equip these vehicles manipulator arms make joint use of arm movement. The also four-wheel rover,...
The genus Endomicrobium is a dominant bacterial group in the gut of lower termites, and most phylotypes are intracellular symbionts protists. Here we report discovery ectosymbionts termite We found that bristle-like cells attached to surface spirotrichosomid protist inhabiting Stolotermes victoriensis. Transmission electron microscopy revealed putative cell likely via protrusion from tip bacterium. A phylotype, sharing 98.9% 16S rRNA sequence identity with protists, was also on Trichonympha...
Almost all examined cockroaches harbor an obligate intracellular endosymbiont, Blattabacterium cuenoti. On the basis of genome content, has been inferred to recycle nitrogen wastes and provide amino acids cofactors for its hosts. Most strains sequenced date a ∼630 kbp, with exception termite Mastotermes darwiniensis (∼590 kbp) Cryptocercus punctulatus (∼614 kbp), representative sister group termites. Such reduction may have led ultimate loss in termites other than Mastotermes. In this study,...
Abstract The Clostridia is a dominant bacterial class in the guts of various animals and are considered to nutritionally contribute animal host. Here, we discovered clostridial endosymbionts cellulolytic protists termite guts, which have never been reported with evidence. We obtained (near-)complete genome sequences three endosymbiotic Clostridia, each associated different parabasalid protist species infection rates: Trichonympha agilis, Pseudotrichonympha grassii, Devescovina sp. All these...
ABSTRACT Four Psychrobacter strains, JCM 18900, 18901, 18902, and 18903, related to either nivimaris or cibarius , were isolated from frozen marine animals. The genome information of these four strains will be useful for studies their physiology adaptation properties conditions.
Brachybacterium sp. strain LB25 produces a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents. The enzyme hydrolyzed starch to produce maltotriose primarily. structural gene encoding the from was cloned and sequenced. amino acid sequence of showed significant similarity (45 49%) amylases genus Streptomyces. expressed Escherichia coli, but specific activity recombinant lower than purified LB25.
The vast majority of prokaryotic species are difficult or impossible to culture in laboratories, which makes it study these organisms using conventional biochemical techniques.