Exosomes are of increasing research interest because they integral to cell-cell communication and implicated in various disease states. Here, we investigated the utility using an ion-depletion zone a microfluidic device concentrate exosomes from culture media four types cell lines. Furthermore, evaluated extent damage following concentration by microchannel compared with concentrated conventional ultra-centrifugation technique. Our results conclusively demonstrate that significantly less is...

We demonstrated a multiplexed fiber Bragg grating (FBG) sensor with static strain resolution of 10 nanostrain (nε) for crustal monitoring. Each unit consists pair identical FBGs sensing and reference, respectively. A narrow linewidth tunable laser is used to interrogate the FBGs, cross-correlation algorithm incorporated demodulate wavelength difference induced by strain. When no applied, an ultrahigh precision corresponding 3.3 nε was obtained, indicating ultimate system. With variable...

This article describes a novel assembly technique with highly accurate semi-automatic alignment of polydimethylsiloxane (PDMS) substrates for microfluidic devices. The is realized by specific concave structures, consisting fringes PDMS named the "Pliant Zipper". Microchannels and Pliant Zipper are easily fabricated together soft lithography. Applying two Zippers on different enables them to be engaged semi-automatically in position. aligned held tightly without any external support. Highly...

The photocured elastomer presented here provided extremely high replication accuracy due to its thermal shrinkage of less than 0.02%, compared 2.91% in the heat-cured elastomer.

In this paper, we report a new method for size-based capturing of suspended particles by specially designed micro fluidic structure named 'vernier walls'. A number in suspension can be captured based on their sizes series gaps between the closest walls, one standing ceiling and other bottom wide microfluidic channel. The dimensions narrow stepwise along flow direction with fixed step size per gap, which determined according to required resolution. Particles are line each corresponding...

Correction for ‘Nanometer-level high-accuracy molding using a photo-curable silicone elastomer by suppressing thermal shrinkage’ Katsuo Mogi <italic>et al.</italic>, <italic>RSC Adv.</italic>, 2015, <bold>5</bold>, 10172–10177.

The development and growth of microfluidics has been mainly based on various novel fabrication techniques for downsizing integration the micro/nano components. Especially, an effective technique three-dimensional structures still continues to be strongly required in order improve device performance, functionality, packing density because conventional lamination-based integrating several two-dimensional components is not enough satisfy requirement. Although printers have a high potential...

Digital microfluidics based on electrowetting dielectric (EWOD) devices has potential as a fundamental technology for the accurate preparation of dangerous reagents, high-speed dispensing rapidly deteriorating and fine adjustment expensive such positron emission tomography (PET). To allow single substrate type EWODs to be practically used in an automatic operation system, we developed dimple structure key technique highly droplet manipulation method. The three-dimensional shape is embossed...

Printed electronics, in which microcircuit patterns are fabricated using printing methods, the focus of considerable attention for fabrication devices such as flat panel displays, solar cells, and flexible electronics. In particular, gravure offset is considered to be a highly promising approach rapid fine with well-controlled thickness. At present, however, because occurrence side etching during metal plates, features dimensions less than 10 µm cannot printed. order overcome this problem,...

The ion depletion zone of concentration polarization has a strong potential to act as an immaterial barrier, separating delicate submicron substances, including biomolecules, without causing physical damage. However, the detailed mechanisms barrier effect remain incompletely understood because it is difficult visualize linked behavior protons, cations, anions, and charged molecules in thin zone. In this study, pH distribution was measured estimate role proton behavior. This done order use...

The vast majority of prokaryotic species are difficult or impossible to culture in laboratories, which makes it study these organisms using conventional biochemical techniques.

Two typical medium replacement methods are employed to culture cells, that is, traditional manual change, wherein complete changes performed at short-term intervals; and perfusion, continuous media delivery discharge performed. The former is associated with the issue of readily accumulation metabolic waste products, which resolved by latter. However, latter requires a specific chamber for cell culturing such as microfluidic channel flow stability. Generally, bio-medical researchers require...

SUMMARY Developing a new concentration technique of virus, which is continuous, quick, easy, mobile, and low cost energy, one the key issue to realize high‐sensitive virus sensing prevent infectious diseases. In this work, we proposed experimentally demonstrated device for using ion depletion zone generated by polarization. The performance method was evaluated fluorescent nanoparticles, baculovirus, albumin, dextran. As result, all samples were successfully concentrated method. We also found...

Cell culture medium replacement is necessary to replenish nutrients and remove waste products, perfusion batch media exchange methods are available. The former can establish an environment similar that in vivo, microfluidic devices frequently used. However, these hampered by incompatibility with commercially available circular dishes the difficulty controlling liquid flow. Here, we fabricated a dish adapter using polydimethylsiloxane has small recess structure for flow control compatible...

We observed the effect of ethanol toxicity on individual cells Botryococcus braunii (B. braunii) in microchannel environment. For continuous observation B. environment, a microfluidic device which has specific channel structure was developed. By using structure, microorganisms were sorted by their size and captured corresponding area channel. In experiment, suspension with DAPI injected into for cell capturing experiment. Following capturing, 0.5% 60 minutes. Damaged confirmed deformation...

Applying high intensity electric field (>MV/m), a non-linear measurement of DNA was conducted by impedance spectroscopy. Low alternate voltage enough to generate strength (0.5 MV/m) in the nanometer gap several micrometer and providing non-faradaic condition. With strength, subjected electrostatic forces, which induced conformational change motion. interaction with alternated would reflect non-liner responses signal. We could differentiate characteristic based on length conformation 100,...

Nanoparticle (NP) concentration is crucial for liquid biopsies and analysis, various NP concentrators (NPCs) have been developed. Methods using ion polarization (ICP), an electrochemical phenomenon based on NPCs consisting of microchannels, attracted attention because samples can be non-invasively concentrated devices with simple structures. The fabrication such limited by the need lithography, requiring special equipment time. To overcome this, we reported a rapid prototyping method...

Delicate animal experiments and microdose clinical trials using short-lived radioisotopes require rapid preparation with high accuracy careful attention to safety within a limited timeframe. We have developed an open-style electrowetting on dielectric (EWOD) device containing dimple structures for the of radiolabelled reagents. The was demonstrated by automatic technetium diethylenetriamine pentaacetate ( 99m Tc-DTPA) chelation efficiency (99.7 ± 0.13%). Additionally, we single-photon...

Abstract An electric double layer is the local distribution of ions created at any liquid and solid interface. The thickness typically in nanometers, so that it dominant nanoscale. Because size nanospace same order magnitude as layer, should be partially/totally filled with an layer. Therefore, investigation electrokinetics inside nanospaces, such nanopores nanochannels, very important for electrical/electrochemical analysis manipulation molecules nanoparticles nanofluidic systems. Here,...

Moth-eye, which is the nanoscale tapered pillars array and has a remarkable anti-reflection property, been paid more attention to be coated on optical materials for high performance surface. However, there are some problems in conventional fabrication method of moth-eye structure, such as labor-intensive mold master nanoimprint fabrication. In order overcome these problems, we demonstrated novel possible apply large area non-flat surfaces at low cost by printing (stamping). The process was...