A5080654989- Insect and Arachnid Ecology and Behavior
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Plant and animal studies
- Plant Parasitism and Resistance
- Machine Learning in Bioinformatics
- Algorithms and Data Compression
- Plant Pathogens and Fungal Diseases
- Animal Nutrition and Physiology
- CRISPR and Genetic Engineering
- Oral Health Pathology and Treatment
- Salivary Gland Disorders and Functions
- Salmonella and Campylobacter epidemiology
- Plant Disease Resistance and Genetics
- Lichen and fungal ecology
- DNA Repair Mechanisms
- Oral microbiology and periodontitis research
- Gene expression and cancer classification
- Metabolomics and Mass Spectrometry Studies
- Clostridium difficile and Clostridium perfringens research
- Indoor Air Quality and Microbial Exposure
- Mycotoxins in Agriculture and Food
- Gut microbiota and health
- Glycosylation and Glycoproteins Research
- Carcinogens and Genotoxicity Assessment
National Institute of Health Sciences
2023-2025
Tokyo Institute of Technology
2015-2023
Wood-feeding lower termites harbour symbiotic gut protists that support the termite nutritionally by degrading recalcitrant lignocellulose. These themselves host specific endo- and ectosymbiotic bacteria, functions of which remain largely unknown. Here, we present draft genomes a dominant, uncultured ectosymbiont belonging to order Bacteroidales, 'Candidatus Symbiothrix dinenymphae', colonizes cell surface cellulolytic Dinenympha spp. We analysed four single-cell Ca. S. dinenymphae, highest...
Abstract Background Bleomycin (Bleo) is a glycopeptide with potent antitumor activity that induces DNA double-strand breaks (DSBs) through free radical generation, similar to ionizing radiation (IR). Therefore, Bleo considered radiomimetic drug. However, differences in repair mechanisms between IR- and Bleo-induced damage have not been fully elucidated. the present study, we examined panel of repair-deficient human TK6 cell lines elucidate relative contributions individual factors. Results...
Abstract The cellulolytic protist Trichonympha agilis in the termite gut permanently hosts two symbiotic bacteria, ‘Candidatus Endomicrobium trichonymphae’ and Desulfovibrio trichonymphae’. former is an intracellular symbiont, latter almost but still connected to outside via a small pore. complete genome of ‘Ca. has previously been reported, we here present (1 410 056 bp), many pseudogenes, retains biosynthetic pathways for various amino acids cofactors, which are partially complementary...
The genus Endomicrobium is a dominant bacterial group in the gut of lower termites, and most phylotypes are intracellular symbionts protists. Here we report discovery ectosymbionts termite We found that bristle-like cells attached to surface spirotrichosomid protist inhabiting Stolotermes victoriensis. Transmission electron microscopy revealed putative cell likely via protrusion from tip bacterium. A phylotype, sharing 98.9% 16S rRNA sequence identity with protists, was also on Trichonympha...
Abstract The Clostridia is a dominant bacterial class in the guts of various animals and are considered to nutritionally contribute animal host. Here, we discovered clostridial endosymbionts cellulolytic protists termite guts, which have never been reported with evidence. We obtained (near-)complete genome sequences three endosymbiotic Clostridia, each associated different parabasalid protist species infection rates: Trichonympha agilis, Pseudotrichonympha grassii, Devescovina sp. All these...
Fungal community analyses in homes have been attracting attention because fungi are now generally considered to be allergens. Currently, these conducted using the culture method, although fungal communities households often contain species that difficult culture. In contrast, next-generation sequencing (NGS) represents a comprehensive, labor- and time-saving approach can facilitate identification. However, reliability of NGS method has not compared method. this study, an attempt demonstrate...
Campylobacter jejuni is one of the leading causes gastrointestinal illness worldwide and mainly transmitted from chicken through food chain. Previous studies have provided increasing evidence that this pathogen can colonize replicate in broiler during its breeding; however, temporal kinetics laying hen are poorly understood. Considering possible interaction between C. gut microbiota, current study was conducted to address dynamics cecum over 40 weeks, with alteration microbiota fatty acid...
Sequence similarity searches have been widely used in the analyses of metagenomic sequencing data. Finding homologous sequences a reference database enables estimation taxonomic and functional characteristics each query sequence. Because current data consist large number nucleotide sequences, time required for sequence account proportion total time. This time-consuming step makes it difficult to perform large-scale analyses. To analyze data, such as those found human oral microbiome, we...
Periodontitis is an inflammation of tooth-supporting tissues, which caused by bacteria in the subgingival plaque (biofilm) and host immune response. Traditionally, pathogens have been investigated using methods such as culturing, DNA probes, or PCR. The development next-generation sequencing made it possible to investigate whole microbiome plaque. Previous studies implicated dysbiosis etiology periodontitis. However, details are still lacking. In this study, we conducted a metagenomic...
Citreoviridin (CTV) is a mycotoxin that produced by Aspergillus terreus, Eupenicillium ochrosalmoneum and Penicillium citreonigrum, CTV has been detected in wide range of cereal grains throughout the world. Furthermore, it especially serious problem regions where rice consumed as staple food. Moreover, well-known yellow toxin, outbreaks beriberi have occurred due to consumption contaminated even recent years. Although biosynthetic genes A. terreus described, those P. citreonigrum remain...
Metagenomic analysis, a technique used to comprehensively analyze microorganisms present in the environment, requires performing high-precision homology searches on large amounts of sequencing data, size which has increased dramatically with development next-generation sequencing. NCBI BLAST is most widely software for searches, but its speed insufficient throughput current DNA sequencers. In this paper, we propose new, high-performance search algorithm that employs two-step seed strategy...
Abstract The Clostridia is a dominant bacterial class in the guts of various animals and are considered to nutritionally contribute animal host. Here, we discovered clostridial endosymbionts cellulolytic protists termite guts, which have never been reported with evidence. We obtained (near-)complete genome sequences three endosymbiotic , each associated different parabasalid protist species infection rates: Trichonympha agilis Pseudotrichonympha grassii Devescovina sp. All these previously...
Mutagenicity, the ability of chemical agents to cause mutations and potentially lead cancer, is a critical aspect substance safety assessment for protecting human health environment. Metabolic enzymes activate multiple mutagens in living organisms, thus vivo animal models provide highly important information evaluating mutagenicity human. Rats are considered suitable as they share similar metabolic pathway with humans processing toxic exhibit higher responsiveness carcinogens than mice. To...
Metagenome techniques allow analyses of microorganisms and their genes present in a given environment without isolation culture. Thus, metagenomics has become broadly applied tool to study various environments elucidate the relationship between diseases host microbiota. With continuous improvement performance genome sequencers, number sequence reads generated increased exponentially; thus, methods for efficient processing such large numbers sequences are required. To this end, we developed...