A5004566812- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Myxozoan Parasites in Aquatic Species
- Pesticide Residue Analysis and Safety
- Fungal Biology and Applications
- Plant and fungal interactions
- Indoor Air Quality and Microbial Exposure
- Fungal and yeast genetics research
- Fungal Plant Pathogen Control
- Microbial Natural Products and Biosynthesis
- Aquatic Invertebrate Ecology and Behavior
- Essential Oils and Antimicrobial Activity
- Microbial infections and disease research
- Carcinogens and Genotoxicity Assessment
- Insect and Pesticide Research
- Listeria monocytogenes in Food Safety
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- Neurogenesis and neuroplasticity mechanisms
- Potato Plant Research
- Plant biochemistry and biosynthesis
- SARS-CoV-2 detection and testing
- Allergic Rhinitis and Sensitization
National Institute of Health Sciences
2016-2025
National Institute of Health Sciences
2013-2025
The University of Tokyo
2003-2011
Tokyo University of Science
2006
Kyushu University
1966
Scope Cereal grains are commonly contaminated with Fusarium mycotoxins and their plant‐derived masked metabolites. The fate of in the human gut is poorly understood. Here we assess metabolism transport glucoside metabolites common trichothecenes (deoxynivalenol, nivalenol, T‐2 toxin) zearalenone compounds (zearalenone, α‐ β‐zearalenol) vitro. Methods results Masked were incubated artificial digestive juices absorption was assessed differentiated Caco‐2/TC7 cells. Colonic studied using fecal...
In almost all eukaryotes, mitochondrial (mt) genes are transmitted to progeny mainly from the maternal parent. The most popular explanation for this phenomenon is simple dilution of paternal mtDNA, because gametes (sperm) much smaller than (egg) and contribute a limited amount mitochondria progeny. Recently, has been challenged in several reports that describe active digestion sperm down-regulation mtDNA replication sperm, proteolysis triggered by ubiquitination. investigation, we visualized...
Dillapiol was isolated from the essential oil of dill as a specific inhibitor aflatoxin G1 production. It inhibited production by Aspergillus parasiticus with an IC50 value 0.15 microM without inhibiting B1 or fungal growth. Apiol and myristicin, congeners dillapiol, showed similar activity values 0.24 3.5 microM, respectively.
Dioctatin A (DotA), a metabolite of Streptomyces, is known to be an inhibitor human dipeptidyl aminopeptidase II. Here, it was found that DotA strongly inhibited aflatoxin production by Aspergillus parasiticus, with IC50 value 4.0 microM. The mycelial growth the fungus not affected addition at concentration 50 microM, but inhibition conidiation observed same concentration. norsolorinic acid, early biosynthetic intermediate aflatoxin, and reduced mRNA levels genes encoding enzymes,...
This study evaluated the ability of selected strains Trichoderma viride, T. viridescens, and atroviride to inhibit mycelium growth biosynthesis mycotoxins deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEN), α-(α-ZOL) β-zearalenol (β-ZOL) by Fusarium culmorum F. cerealis. For this purpose, an in vitro experiment was carried out on solid substrates (PDA rice). After 5 days co-culture, it found that all used significantly inhibited mycelium. Qualitative assessment pathogen-antagonist...
Toxicological information on moniliformin (MON), an emerging mycotoxin, is limited. This study examined the acute and 28-day toxicity of orally administered MON in male ICR mice. Regarding toxicity, among single oral doses 0, 20, 40, 80 mg/kg body weight (BW), caused proximal tubular necrosis kidneys at ≥ 40 BW, lethal dose 50 value was estimated as 68.1 BW. 10, BW/day, increased absolute heart but histopathological changes were not evident heart. In contrast, BW induced centrilobular liver...
In Japan, serious food poisoning among individuals who took supplement tablets for lowering plasma cholesterol levels have been publicized since late March 2024. The were prepared from red yeast rice (RYR), a product of Monascus pilosus. Puberulic acid (PA) was detected as an unexpected compound in that caused poisoning. We conducted on-site investigation at the RYR production factory to determine cause PA contamination tablets. Fungi capable producing wipe samples and identified Penicillium...
Trichothecene mycotoxins such as nivalenol and deoxynivalenol frequently contaminate foodstuffs. Recently, several trichothecene glucosides have been found in trichothecene-contaminated foods, information about their chemistry, toxicity, occurrence is required. In this study, a glucoside of was isolated from nivalenol-contaminated wheat identified nivalenol-3-O-β-D-glucopyranoside. Analytical methods using multifunctional column or an immunoaffinity developed for the simultaneous...
Inhibitors of deoxynivalenol production by Fusarium graminearum are useful for protecting crops from contamination. We isolated precocenes and piperitone the essential oils Matricaria recutita Eucalyptus dives, respectively, as specific inhibitors 3-acetyldeoxynivalenol, a biosynthetic precursor deoxynivalenol. Precocenes I II inhibited 3-acetyldeoxynivalenol F. in liquid culture with IC50 values 16.6, 1.2, 306 μM, without inhibiting fungal growth. Precocene also fungus solid on rice an...
There is a strain of Clostridium perfringens, W5052, which does not produce known enterotoxin. We herein report that the W5052 expressed homologue iota-like toxin components sa and sb C. spiroforme, named perfringens enterotoxin, CPILE-a CPILE-b, respectively, based on results genome sequencing analysis systematic protein screening. In nicotinamide glyco-hydrolase (NADase) assay hydrolysis activity was dose-dependently increased by concentration rCPILE-a, as judged mass spectrometry...
The contamination levels of beauvericin and four enniatins, A, A1, B B1, in 207 samples wheat flour corn grits on the Japanese market were determined by an analytical method based LC-MS/MS. toxins extracted from with acetonitrile-water (85:15, v/v) then purified C18 cartridges. was validated a single laboratory using spiked at two levels; recovery five ranged 91.1% to 113.8%. Enniatin frequently detected imported (81.8%) domestic (85.6%), highest concentration enniatin present sample (633 μg...
Small grain cereals are frequently infected with mycotoxigenic Fusarium fungi. Oats have a particularly high risk of contamination type A trichothecene mycotoxins; their glucoside conjugates also been reported. Agronomy practices, cereal variety and weather conditions suggested to play role in infection oats. The current study investigates concentrations free conjugated mycotoxins organic conventional oats grown Scotland. In 2019, 33 milling oat samples (12 organic, 21 conventional) were...
The determination of important regulated mycotoxins in rice has been reported previously but not the individual matrix white, brown, red, and basmati with respect to effect, recovery, stability. A total 190 Asian rices were examined for mycotoxin contamination by LC-ESI-MS/MS method. Significant variation (p < 0.05) effect was observed fumonisins. Methanol improved limits detection (LOD) HT-2 from 50 μg/kg 2.3 promoting ionization efficiency ammonium-adduct. LOD quantitation ranged 0.1 18...
The most common diagnostic method used for coronavirus disease-2019 (COVID-19) is real-time reverse transcription polymerase chain reaction (PCR). However, it requires complex and labor-intensive procedures involves excessive positive results derived from viral debris. We developed a the direct detection of severe acute respiratory syndrome 2 (SARS-CoV-2) in nasopharyngeal swabs, which uses matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-ToF MS) to...
Eighty-seven samples of malt from several Polish malting plants and 157 beer the available on market (in 2018) were tested for Fusarium mycotoxins (deoxynivalenol (DON), nivalenol (NIV)), their modified forms ((deoxynivalenol-3-glucoside (DON-3G), nivalenol-3-glucoside (NIV-3G), 3-acetyldeoxynivalenol (3-AcDON)). DON its metabolite, DON-3G, found most, among analyzed; DON-3G present in 90% 91% samples, 97% 99% respectively. NIV was 24% 64% NIV-3G 48% 39% samples. In mean concentration 52.9...
Enniatins are emerging mycotoxins that contaminate foods. The present study investigated the oral pharmacokinetics and 28-day repeated-dose toxicity of enniatin B (ENNB) in CD1 (ICR) mice. In pharmacokinetic study, male mice received a single or intravenous dose ENNB [30 mg/kg body weight (BW) 1 BW, respectively]. After dosing, exhibited 139.9% bioavailability, 5.1-h elimination half-life, 5.26% fecal excretion from 4 to 24 h post-dose, upregulation Cyp7a1, Cyp2a12, Cyp2b10, Cyp26a1 liver 2...