A5037814992- Myxozoan Parasites in Aquatic Species
- Microbial infections and disease research
- Mycotoxins in Agriculture and Food
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Immune Response and Inflammation
- Advanced MRI Techniques and Applications
- Aquatic Invertebrate Ecology and Behavior
- Listeria monocytogenes in Food Safety
- Body Composition Measurement Techniques
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Pesticide Residue Analysis and Safety
- Toxoplasma gondii Research Studies
- Antimicrobial Peptides and Activities
- Escherichia coli research studies
- Food Safety and Hygiene
- Gallbladder and Bile Duct Disorders
- Probiotics and Fermented Foods
- Renal Diseases and Glomerulopathies
- Cardiac Imaging and Diagnostics
- Marine Ecology and Invasive Species
- MRI in cancer diagnosis
- NF-κB Signaling Pathways
National Institute of Health Sciences
2015-2025
National Institute of Health Sciences
2012-2024
Japan Sport Council
2017-2023
Ise Red Cross Hospital
1999-2019
National Institutes of Health
2016
Kansai University
2013
National Institute of Infectious Diseases
2012
University of Miyazaki
1994-2010
Public Health Department
2010
Siemens (Germany)
2008-2009
Background. Outbreaks of an unidentified food-borne illness associated with the consumption raw fish have increased in Japan since 2003. Those affected this develop diarrhea and emesis within 2–20 hours after a meal including fish. No known causative agents such as bacteria, viruses, bacterial toxins, or toxic chemicals been detected foods that were ingested. Fortunately, is self-limiting good prognosis all cases.
We examined the effect of curcumin (CUR) ingestion before or after exercise on changes in muscle damage and inflammatory responses exercise. conducted two parallel experiments with different CUR timings using a double-blind crossover. In Exp. 1, ten healthy men ingested 180 mg d-1 placebo (PLA) 7 days 2, other PLA They performed 30 maximal isokinetic (120°s-1 ) eccentric contractions elbow flexors an dynamometer, this was repeated arm ≥4 weeks later. Maximal voluntary contraction (MVC)...
Helicobacter pylori is recognized as an etiologic agent of gastroduodenal diseases. Among toxic substances produced by H. pylori, LPS exhibits extremely low endotoxic activity compared to the typical LPSs, such that Escherichia coli. We found LPS-low-responder stomach cancer cell line MKN28, which expresses Toll-like receptor 4 (TLR4) at levels, showed similar levels interleukin-8 (IL-8) induction or E. coli preparations. Weak IL-8 preparations was suppressed expression a dominant negative...
In Japan, serious food poisoning among individuals who took supplement tablets for lowering plasma cholesterol levels have been publicized since late March 2024. The were prepared from red yeast rice (RYR), a product of Monascus pilosus. Puberulic acid (PA) was detected as an unexpected compound in that caused poisoning. We conducted on-site investigation at the RYR production factory to determine cause PA contamination tablets. Fungi capable producing wipe samples and identified Penicillium...
Regions of mouse CD14 required for Toll-like receptor 2 (TLR2)- and TLR4-mediated activation NF-kappaB were studied in transiently transfected 293 cells. Wild-type enhanced lipopolysaccharide (LPS)-induced NF-kappaB-dependent reporter activity cells expressing TLR4/MD-2, deletion amino acid regions 35-44, 144-153, 235-243, 270-275 impaired the activation. Unlike human CD14, truncated at 151 lost activity. Deletion acids 35-44 or 235-243 also abrogated TLR2-mediated NF-kappaB, whereas mutants...
ABSTRACT MD-2 has been reported to be required for the translocation of Toll-like receptor 4 (TLR4) cell surface. However, mechanism by which promotes TLR4 is unknown. We identified presence two forms with different molecular masses (approximately 110 and 130 kDa) when was expressed together MD-2. Expressing alone produced only 110-kDa form. Using a membrane-impermeable biotinylation reagent, we found that 130-kDa form on When cellular extract prepared from cells expressing treated N...
MD-2 is physically associated with Toll-like receptor 4 (TLR4) and required for TLR4-mediated LPS signaling. Western blotting analysis revealed the presence of three forms human (h)MD-2 different electrophoretic mobilities. After N-glycosidase treatment cellular extract prepared from cells expressing hMD-2, only a single form fastest mobility was detected. Mutation either one two potential glycosylation sites (Asn(26) Asn(114)) resulted in disappearance slowest form, detected hMD-2 carrying...
Kudoa septempunctata is a myxosporean parasite of Paralichthys olivaceus (olive flounder) and causes foodborne illness that affects more than 100 cases in Japan each year. We previously reported the consumption raw olive flounder meat containing high concentration K. spores induces transient but severe diarrhea emesis through an unknown mechanism. Here, we demonstrate sporoplasm plays important role mediating toxicity septempunctata. When were inoculated Caco-2 human intestinal cells,...
Trichothecene mycotoxins such as nivalenol and deoxynivalenol frequently contaminate foodstuffs. Recently, several trichothecene glucosides have been found in trichothecene-contaminated foods, information about their chemistry, toxicity, occurrence is required. In this study, a glucoside of was isolated from nivalenol-contaminated wheat identified nivalenol-3-O-β-D-glucopyranoside. Analytical methods using multifunctional column or an immunoaffinity developed for the simultaneous...
Abstract Purpose To confirm the superiority of navigator‐triggered prospective acquisition correction (PACE) technique over conventional respiratory‐triggered (RESP) technique, something that has been perceived experimentally but without definite evidence, for free‐breathing three‐dimensional (3D) magnetic resonance cholangiopancreatography (MRCP) using healthy volunteers. Materials and Methods Free‐breathing 3D turbo spin‐echo MRCP both PACE RESP techniques were prospectively performed on...
Fabry disease is an X-linked recessive inborn metabolic disorder caused by a deficiency of the lysosomal enzyme alpha-galactosidase A (EC 3.2.1.22). The causative mutations are diverse, include both large rearrangements and single-base substitutions, dispersed throughout 7 exons gene (GLA). Mutation hotspots for do not exist. We examined 62 patients in Japan found 24 GLA mutations, including 11 novel ones. potential treatment reported active site specific chaperone (ASSC) therapy using...
The National Food Surveillance System in Japan was formed 1998 to monitor the contamination of retail foods with bacterial pathogens. Approximately 2000-3000 samples were tested annually, and data from food categories that had more than 400 collected during 1998-2008 analysed. With regard meat, frequency positive for Salmonella chicken raw consumption ground 12.7% 33.5%, respectively. Moreover, Shiga toxin-producing Escherichia coli (STEC) O157 found organ meat processed although at a low...
An unidentified foodborne disease associated with the consumption of raw fresh fish was noticed from 1999 in West Japan. In 2010, a novel multivalvulid parasite, Kudoa septempunctata (Myxozoa: Myxosporea) discovered as being causative agent this and Ministry Health, Labour Welfare Japan (MHLW) named "Kudoa food poisoning". is myxosporean 6–7 polar capsules shell valves spore. The life-cycle K. has not been elucidated yet. However, it probably involves an alternative invertebrate host such...
Background:The aim of this study was to assess the echocardiographic characteristics chronic hemodialysis (HD) patients with end-stage renal disease (ESRD) in a multicenter prospective cohort study.
The Myxozoa are oligo-cellular parasites with alternate hosts—fish and annelid worms—and some myxozoan species harm farmed fish. phylum Myxozoa, comprising 2,100 species, was difficult to position in the tree of life, due its fast evolutionary rate. Recent phylogenomic studies utilizing an extensive number nuclear-encoded genes have confirmed that Myxozoans belong Cnidaria. Nevertheless, evolution parasitism extreme body simplification is not well understood, no mitochondrial DNA sequence...
The contamination levels of beauvericin and four enniatins, A, A1, B B1, in 207 samples wheat flour corn grits on the Japanese market were determined by an analytical method based LC-MS/MS. toxins extracted from with acetonitrile-water (85:15, v/v) then purified C18 cartridges. was validated a single laboratory using spiked at two levels; recovery five ranged 91.1% to 113.8%. Enniatin frequently detected imported (81.8%) domestic (85.6%), highest concentration enniatin present sample (633 μg...
ABSTRACT Salmonella lipid A is inactive in human macrophages despite being potently active murine macrophages. We investigated the molecular basis for this species-specific action of A. When CD14 (mCD14), mTLR4, and mMD-2 were all expressed monocytic THP-1 cells, these cells capable responding to Expressing each proteins separately did not impart such responsiveness. Expression mTLR4 plus mMD-2, but mCD14 or conferred In expressing mCD14, replacing with had no effect on responsiveness...
Many microorganisms produce extracellular polymers referred to collectively as "slime" or glycocalyx, and form biofilms on solid surfaces in natural ecosystems. Campylobacter jejuni, one of the most important foodborne pathogens, also has ability biofilm stainless steel, glass, polyvinyl chloride vitro. However, issue formation by species not been extensively examined. The present study was performed examine mode adhesion C. jejuni a smooth surface. When bacterial suspensions Brucella broth...
Raw fish consumption is increasing worldwide. Since around the year 2000, western regions of Japan have reported a foodborne disease unknown cause that occurred after flounder. In October 2010, particularly large outbreak was in these among individuals who consumed flounder had been raised aquaculture systems. The median incubation period 5 h (range, 4-19 h), and most frequently symptom diarrhea (80%). risk estimate significantly higher than development symptoms (odds ratio=9.50; 95%...