A5058438038- Enzyme Structure and Function
- Research Data Management Practices
- Glutathione Transferases and Polymorphisms
- Enzyme Production and Characterization
- Data Quality and Management
- Scientific Computing and Data Management
- Cancer Research and Treatments
- Steroid Chemistry and Biochemistry
- Amino Acid Enzymes and Metabolism
- Housing Market and Economics
- Microbial Metabolic Engineering and Bioproduction
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Microbial Natural Products and Biosynthesis
- Biopolymer Synthesis and Applications
- Plant biochemistry and biosynthesis
- Microbial Metabolites in Food Biotechnology
- Synthesis and Characterization of Heterocyclic Compounds
- Biomedical Text Mining and Ontologies
- S100 Proteins and Annexins
- Phytochemistry and Bioactivity Studies
- Microbial bioremediation and biosurfactants
- Microbial Metabolism and Applications
- Peptidase Inhibition and Analysis
University of Illinois Urbana-Champaign
2014-2024
University of Arizona
2024
University of Illinois System
2020
Harvard University
2009-2015
The Ohio State University
2008
Albert Einstein College of Medicine
2007
University of Notre Dame
2007
United States Geological Survey
2004
Nonribosomal peptide synthetase (NRPS) assembly lines are major avenues for the biosynthesis of a vast array peptidyl natural products. Several hundred bacterial NRPS gene clusters contain small (∼70-residue) protein belonging to MbtH family which no function has been defined. Here we show that two strictly conserved Trp residues in MbtH-like proteins contribute stimulation amino acid adenylation some modules. We also demonstrate can be stimulated not only by cognate but homologues from...
We measured nitrification rates in sediment samples collected from a variety of aquatic habitats Navigation Pool 8 the Upper Mississippi River (UMR) 7 times between May 2000 and October 2001. also conducted nutrient-enrichment experiments analyzed vertical profiles to determine factors regulating nitrification. Nitrification were relatively high compared other ecosystems (ranging 0–8.25 μg N cm−2 h−1) exhibited significant temporal spatial patterns. greatest during summer spring autumn...
The rapid advance in genome sequencing presents substantial challenges for protein functional assignment, with half or more of new sequences inferred from these genomes having uncertain assignments. assignment enzyme function functionally diverse superfamilies represents a particular challenge, which we address through combination computational predictions, enzymology, and structural biology. Here describe the results focused investigation group enzymes enolase superfamily that are involved...
The reaction catalyzed by orotidine 5'-monophosphate decarboxylase (OMPDC) involves a stabilized anionic intermediate, although the structural basis for rate acceleration (k(cat)/k(non), 7.1 x 10(16)) and proficiency [(k(cat)/K(M))/k(non), 4.8 10(22) M(-1)] is uncertain. That OMPDCs from Methanothermobacter thermautotrophicus (MtOMPDC) Saccharomyces cerevisiae (ScOMPDC) catalyze exchange of H6 UMP product with solvent deuterium allows an estimate lower limit on associated stabilization...
Funders increasingly require that data sets arising from sponsored research must be preserved and shared, many publishers either or encourage accompanying articles are made available through a publicly accessible repository. Additionally, researchers wish to make their regardless of funder requirements both enhance impact also propel the concept open science. However, curation activities support these preservation sharing costly, requiring advanced practices, training, specific technical...
d-Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the most abundant enzyme, is paradigm member of recently recognized mechanistically diverse RuBisCO superfamily. The reaction initiated by abstraction proton from C3 d-ribulose substrate a carbamate oxygen carboxylated Lys 201 (spinach enzyme). Heterofunctional homologues found in species Bacilli catalyze tautomerization ("enolization") 2,3-diketo-5-methylthiopentane 1-phosphate (DK-MTP 1-P) methionine salvage pathway which...
Syringolins are a class of cyclic tripeptide natural products that potent and irreversible inhibitors the eukaryotic proteasome. In addition to being hybrid NRPS/PKS molecules, they also feature an unusual ureido-linkage (red) between two amino acid monomers. Here we report first in vitro characterization enzymatic formation which is catalyzed by NRPS, SylC. Using (13)C- (18)O-labeling studies, show biosynthesis occurs via N-carboxylation form initial N-carboxy-aminoacyl-S-Ppant enzyme...
The Pseudomonas aeruginosa toxin L-2-amino-4-methoxy-trans-3-butenoic acid (AMB) is a non-proteinogenic amino which toxic for prokaryotes and eukaryotes. Production of AMB requires five-gene cluster encoding putative LysE-type transporter (AmbA), two nonribosomal peptide synthetases (AmbB AmbE), iron(II)/α-ketoglutarate-dependent oxygenases (AmbC AmbD). Bioinformatics analysis predicts one thiolation (T) domain AmbB T domains (T1 T2) AmbE, suggesting that generated by processing step from...
INTRODUCTION Data curation may be an emerging service for academic libraries, but researchers actively “curate” their data in a number of ways—even if terminology not always align. Building on past userneeds assessments performed via survey and focus groups, the authors sought direct input from importance utilization specific activities. METHODS Between October 21, 2016, November 18, study team held groups with 91 participants at six different institutions to determine which activities were...
<strong>Objective</strong>: Many academic and research institutions are exploring opportunities to better support researchers in sharing their data. As partners the Data Curation Network project, our six developed a comparison of current levels provided for meet data goals through library-based repository curation services. <strong>Methods</strong>: Each institutional lead written summary services based on previously structure, followed by group discussion refinement descriptions. Service...
Online resources enable unfettered access to and analysis of scientific data are considered crucial for the advancement modern science. Despite clear power online resources, including web-available databases, proliferation can be problematic due challenges in sustainability long-term persistence. As areas research become increasingly dependent on collections data, an understanding community’s capacity develop maintain such is needed. The advent Internet coincided with expanding adoption...
Data sharing and reuse are regarded as important components of the research workflow key elements in open science. While is well-documented some circumstances, utility data for all domains less clear, limited evidence wide-spread demand can make it challenging to justify effort funds required format, document, share, preserve data. This paper describes a project that: (1) surveyed authors highly cited papers published 2015 at University Illinois Urbana-Champaign nine STEM disciplines...
Some homologues of d-ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) do not catalyze carboxylation and are designated RuBisCO-like proteins (RLPs). The RLP from Rhodospirillum rubrum (gi:83593333) catalyzes a novel isomerization reaction (overall 1,3-proton transfer reaction; likely, two 1,2-proton reactions) that converts 5-methylthio-d-ribulose 1-phosphate to 3:1 mixture 1-methylthioxylulose 5-phosphate 1-methylthioribulose 5-phosphate. Disruption the gene encoding abolishes...
<strong>Objective and Setting</strong>: While data management planning becomes more commonplace, moving from into implementation remains a hurdle for many researchers. With little specific guidance funding agencies libraries in the early stages of developing services to assist researchers, insights what contributes successful are sorely needed. The objective this study was document how multidisciplinary research team, after consultation with University Illinois Library, took steps implement...
Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P isomerized 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) reductively dethiomethylated 1-deoxy-d-xylulose (DXP), an intermediate nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., press]....
The CODATA Data Science Journal is a peer-reviewed, open access, electronic journal, publishing papers on the management, dissemination, use and reuse of research data databases across all domains, including science, technology, humanities arts. scope journal includes descriptions systems, their implementations publication, applications, infrastructures, software, legal, reproducibility transparency issues, availability usability complex datasets, with particular focus principles, policies...
Long-term agricultural experiments are essential to measure the impacts of farming practices on crop yields, soil fertility and biogeochemical processes. However, these often only manifest at decadal timescales, requiring committed consistent data collection that exceeds timelines for most experiments. The second oldest experiment in world, Morrow Plots University Illinois Urbana-Champaign (USA) has examined impact rotation treatments maize (Zea mays L.) yields since 1876. While results have...