A5086949607- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Genomics and Chromatin Dynamics
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Soybean genetics and cultivation
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Long-Term Effects of COVID-19
- Plant Micronutrient Interactions and Effects
- Religion, Spirituality, and Psychology
- Seed Germination and Physiology
- Metalloenzymes and iron-sulfur proteins
- Dermatological and COVID-19 studies
- Protist diversity and phylogeny
- Ubiquitin and proteasome pathways
Universidad Autónoma de Madrid
2004-2023
Universidad Politécnica de Madrid
2016-2023
Centre for Plant Biotechnology and Genomics
2016-2023
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2016-2023
Instituto de Física Teórica
2023
Hospital Universitario HM Sanchinarro
2023
Centro de Biología Molecular Severo Ochoa
2004-2016
Universidad de Granada
2014
University of California, Los Angeles
2010-2012
University of Castilla-La Mancha
2011
Climate change is increasing the frequency of extreme heat events that aggravate its negative impact on plant development and agricultural yield. Most experiments designed to study adaption stress apply homogeneous high temperatures both shoot root. However, this treatment does not mimic conditions in natural fields, where roots grow a dark environment with descending temperature gradient. Excessively severely decrease cell division root meristem, compromising growth, while quiescent center...
In eukaryotic cells, the function of DNA replication licensing components (Cdc6 and Cdt1, among others) is crucial for cell proliferation genome stability. However, little known about their role in whole organisms whether control interfaces with differentiation developmental programs. Here, we study Arabidopsis thaliana CDT1, its regulation, consequences overriding control. The availability AtCDT1 strictly regulated at two levels: (1) transcription level, by E2F growth-arresting signals, (2)...
The relationship between epigenetic marks on chromatin and the regulation of DNA replication is poorly understood. Mutations H3K27 methyltransferase genes, ARABIDOPSIS TRITHORAX-RELATED PROTEIN5 (ATXR5) ATXR6, result in re-replication (repeated origin firing within same cell cycle). Here we show that mutations reduce methylation act to suppress phenotype atxr5 atxr6 mutants. This suggests methylation, a mark enriched at heterochromatic regions re-replicate atxr5/6 mutants, required for...
In eukaryotic cells, environmental and developmental signals alter chromatin structure modulate gene expression. Heterochromatin constitutes the transcriptionally inactive state of genome in plants mammals is generally characterized by DNA methylation histone modifications such as H3 lysine 9 (H3K9) methylation. Arabidopsis thaliana, H3K9 are usually colocated set up a mutually self-reinforcing stable state. Here, contrast, we found that SUVR5, plant Su(var)3–9 homolog with SET...
Abscisic acid (ABA) is fundamental for plant development. Multiple factors have been identified that participate in the ABA signaling network, although a role of many proteins still await to be demonstrated. Here we investigated GEM (GL2 EXPRESSION MODULATOR), originally annotated as an ABA-responsive protein. contains GRAM domain, feature shared with other eight Arabidopsis which propose name GRE (GEM-RELATED) proteins. We found (i) expression responds ABA, (ii) its promoter ABRE sites...
The generation of nitrogen fixing crops is considered a challenge that could lead to new agricultural 'green' revolution. Here, we report the use synthetic biology tools achieve and optimize production active nitrogenase Fe protein (NifH) in chloroplasts tobacco plants. Azotobacter vinelandii fixation genes, nifH, M, U S, were re-designed for accumulation cells. Targeting chloroplast was optimized by screening identifying minimal length transit peptides performing properly each specific Nif...
There is a need for the development of synthetic biology methods and tools to facilitate rapid efficient engineering yeast that accommodates needs specific biotechnology projects. In particular, manipulation mitochondrial proteome has interesting potential applications due its compartmentalized nature. One these advantages resides in fact metalation occurs after protein import into mitochondria, which contains pools iron, zinc, copper manganese ions can be utilized recombinant metalloprotein...
Summary DNA methylation is an important epigenetic mechanism for controlling innate immunity against microbial pathogens in plants. Little known, however, about the manner which viral infections interact with pathways. Here we investigate crosstalk between silencing and Arabidopsis inflorescences. We found that tobacco rattle virus (TRV) causes changes expression of key transcriptional gene factors RNA‐directed activities coincide at whole genome level. Viral susceptibility/resistance was...
Measure Yourself Concerns and Wellbeing (MYCaW) is a patient-centered questionnaire that allows cancer patients to identify quantify the severity of their "concerns" "wellbeing," as opposed using predetermined list. MYCaW administration brief aids in prioritizing treatment approaches. Our goal was assess convergent validity responsiveness scores over time, generalizability existing qualitative coding framework different complementary integrative oncology settings content validity.Baseline...
For more than 20 years, plant biologists have tried to achieve complete control of transgene expression. Until the techniques target transgenes safe harbor sites in genome become routine, flanking with genetic insulators, DNA sequences that create independent domains gene expression, can help avoid positional effects and stabilize their We have, for first time, compared effect three insulator previously described literature one never tested before. Our results indicate use increases but only...
Virulence, the effect of pathogen infection on progeny production, is a major determinant host and fitness as it affects fecundity transmission. In plant–virus interactions, ample evidence indicates that virulence genetically controlled by both partners. However, genetic determinants are poorly understood. Through genome-wide association study (GWAS) 154 Arabidopsis thaliana genotypes infected Cucumber mosaic virus (CMV), we identified eight genes associated with virulence, most them...
Most cellular proteins involved in genome replication are conserved all eukaryotic lineages including yeast, plants and animals. However, the mechanisms controlling their availability during cell cycle less well defined. Here we show that Arabidopsis encodes for two ORC1 highly similar amino acid sequence have partially overlapping expression domains but with distinct functions. The ancestral ORC1b gene, present before partial duplication of genome, has retained canonical function DNA...
Obtaining high and stable transgene expression is of vital importance for plant genetic engineering. A lot known about the relationship between terminator efficiency gene expression, but no studies have addressed usage stability or heritable silencing. In this paper, we aim to analyze if terminators are a determining factor in establishment promoter DNA methylation transgenes. Our experiments comparing plants with LUC reporter under 35S CaMV good (Thsp, T35S) show that use efficient...
Background Managing the inflammatory response to SARS-Cov-2 could prevent respiratory insufficiency. Cytokine profiles identify cases at risk of severe disease. Methods We designed a randomized phase II clinical trial determine whether combination ruxolitinib (5 mg twice day for 7 days followed by 10 BID days) plus simvastatin (40 once 14 days), reduce incidence insufficiency in COVID-19. 48 cytokines were correlated with outcome. Participants Patients admitted due COVID-19 infection mild...
Nucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions the translocon complexes at outer (TOC) and inner (TIC) membranes. These exist in multiple isoforms higher plants show differential specificity tissue abundance. While some for photosynthesis-related precursor proteins, others distinctly recognize non-photosynthetic housekeeping proteins. Here we used TPs from four Arabidopsis thaliana...
Cell division and cell fate decisions are highly regulated processes that need to be coordinated both spatially temporally for correct plant growth development. Gaining a deeper molecular cellular understanding of these links is especially relevant biology since, unlike in animals, formation new organs process takes place after embryogenesis continues throughout the entire lifespan. The recent identification novel factor, GEM, has provided framework coordinates Arabidopsis epidermis. GEM an...