A5019061307- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Chromosomal and Genetic Variations
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Aquaculture disease management and microbiota
- Seed Germination and Physiology
- DNA Repair Mechanisms
- Enzyme Production and Characterization
- Light effects on plants
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Plant and soil sciences
- Plant Stress Responses and Tolerance
- Genomics and Chromatin Dynamics
- Parvovirus B19 Infection Studies
- Plant responses to water stress
- Microtubule and mitosis dynamics
- Bacterial Identification and Susceptibility Testing
Universidad Nacional Autónoma de México
2014-2024
Instituto de Ecología
2013-2024
Centro de Edafología y Biología Aplicada del Segura
2024
Universidad Autónoma de la Ciudad de México
2019
Instituto de Biomedicina de Sevilla
2011-2018
Universidad de Sevilla
2018
Universidad Autónoma de Madrid
2004-2017
Centro de Biología Molecular Severo Ochoa
2008-2017
Consejo Superior de Investigaciones Científicas
2008-2017
University of Puerto Rico System
2017
Myeloid-derived suppressor cells (MDSC) promote tumor growth by blocking anti-tumor T cell responses. Recent reports show that MDSC increase fatty acid uptake and oxidation (FAO) to support their immunosuppressive functions. Inhibition of FAO promoted a therapeutic cell-mediated effect. Here, we sought determine the mechanisms which tumor-infiltrating exogenous lipids undergo metabolic functional reprogramming become highly cells. The results showed tumor-derived cytokines (G-CSF GM-CSF)...
ABSTRACT Aeromonas hydrophila is an opportunistic pathogen and the leading cause of fatal hemorrhagic septicemia in rainbow trout. A gene encoding elastolytic activity, ahyB , was cloned from AG2 into pUC18 expressed Escherichia coli nonproteolytic species salmonicida subsp. masoucida . Nucleotide sequence analysis revealed open reading frame 1,764 nucleotides with coding capacity for a 588-amino-acid protein molecular weight 62,728. The first 13 N-terminal amino acids purified protease...
Control of gene expression depends on a complex and delicate balance various posttranslational modifications histones. However, the relevance specific combinations histone is not fully defined. Downstream effector proteins recognize particular transduce this information into patterns. Methylation H3 at lysine 4 (H3K4me) landmark control in eukaryotes. Its recognition presence protein motif termed plant homeodomain (PHD) that specifically binds to H3K4me3. Here, we establish Arabidopsis ORC1,...
MADS-domain transcription factors play pivotal roles in numerous developmental processes Arabidopsis thaliana. While their involvement flowering transition and floral development has been extensively examined, functions root remain relatively unexplored. Here, we explored the function genetic interaction of three MADS-box genes ( XAL2 , SOC1 AGL24 ) primary development. By analyzing loss-of-function overexpression lines, found that both critical components transition, redundantly act as...
Abstract The cell cycle is the series of molecular events that allows cells to duplicate and segregate their chromosomes form new cells. finding a protein kinase, product yeast cdc2 gene, was fundamental in regulation G 2 /M 1 /S transitions, associated with unstable proteins named cyclins, opened very exciting dynamic research area. number gene products participate development may be hundreds, there high degree conservation sequences regulatory pathways among eukaryotes. Although are clear...
Synthetic oligonucleotide primers of 24 and 23 bases were used in a PCR assay to amplify sequence the lip gene, which encodes thermostable extracellular lipase Aeromonas hydrophila. A DNA fragment approximately 760 bp was amplified from both sources, i.e., lysed A. hydrophila cells isolated DNA. The detected ethidium bromide-stained agarose gels or by Southern blot analysis with an internal HindIII-BamHI 356-bp as hybridization probe. With cells, sensitivity < 10 CFU, target, lower...
Cell cycle control is fundamental in eukaryotic development. Several modeling efforts have been used to integrate the complex network of interacting molecular components involved cell dynamics. In this paper, we aimed at recovering regulatory logic upstream previously known control, with aim understanding mechanisms underlying emergence cyclic behavior such components. We focus on Arabidopsis thaliana, but given that many regulation are conserved among eukaryotes, when experimental data for...
Summary Root knot nematodes ( RKN s) penetrate into the root vascular cylinder, triggering morphogenetic changes to induce galls, de novo formed ‘pseudo‐organs’ containing several giant cells GC s). Distinctive gene repression events observed in early gall/ GCs development are thought be mediated by post‐transcriptional silencing via microRNAs (mi RNA s), a process that is far from being fully characterized. Arabidopsis thaliana backgrounds with altered activities based on target 35S::...
ABSTRACT The gap gene of Staphylococcus aureus , encoding glyceraldehyde-3-phosphate dehydrogenase, was used as a target to amplify 933-bp DNA fragment by PCR with pair primers 26 and 25 nucleotides in length. products, detected agarose gel electrophoresis, were also amplified from 12 spp. analyzed previously. Hybridization an internal 279-bp probe positive all PCR-positive samples. No products when other gram-positive gram-negative bacterial genera using the same primers. Alu I digestion...
ABSTRACT Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 spp. by PCR. In present study, Alu I digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 be identified with high specificity.
The proliferating cell nuclear antigen (PCNA) is a protein factor required for processive DNA synthesis that associated with G1 cycle proteins. It has been demonstrated previously that, in germinating maize (Zea mays) embryonic axes, PCNA forms complexes two Cdk-A proteins (32 and 36 kDa) putative D-type cyclin. These exhibit kinase activity on histone H1 the homologue of pRB (retinoblastoma) protein. Flow cytometry used to study influence phytohormones benzyladenine (BA) abscisic acid (ABA)...