A5065363214- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Food composition and properties
- Advanced Proteomics Techniques and Applications
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Microbial Metabolites in Food Biotechnology
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Plant Reproductive Biology
- Light effects on plants
- ATP Synthase and ATPases Research
- Plant Genetic and Mutation Studies
- Plant responses to water stress
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Plant and animal studies
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Legume Nitrogen Fixing Symbiosis
- Polyamine Metabolism and Applications
University of Zurich
1995-2024
University of California, Los Angeles
2014-2023
American Society of Plant Biologists
2022
Planta
2021
ETH Zurich
2008-2019
Board of the Swiss Federal Institutes of Technology
2011-2016
Institute of Molecular and Cell Biology
2014
In-Q-Tel
2011
Philip Morris International (United States)
2010
University of Neuchâtel
2010
Abstract Starch contains phosphate covalently bound to the C6-position (70 80% of total phosphate) and C3-position (20 30%) glucosyl residues amylopectin fraction. In plants, transient phosphorylation starch renders granule surface more accessible glucan hydrolyzing enzymes is required for proper degradation. Phosphate also confers desired properties starch-derived pastes industrial applications. Arabidopsis thaliana, removal by phosphatase Excess4 (SEX4) essential breakdown. We identified a...
Regulation of tetrapyrrole biosynthesis in higher plants has been attributed to negative feedback control. Two effectors inhibition have identified, heme and the FLU protein. Inhibition by implicates Fe-branch via regulation initial step synthesis. In present work a FLU-containing chloroplast membrane complex was that besides comprises four enzymes catalyzing final steps chlorophyll The results support notion links synthesis target control, glutamyl-tRNA reductase, thereby allowing also...
Abstract Import of nuclear-encoded precursor proteins from the cytosol is an essential step in chloroplast biogenesis that mediated by protein translocon complexes at inner and outer envelope membrane (TOC). Toc159 thought to be main receptor for photosynthetic proteins, but lacking a large-scale systems approach, this hypothesis has only been tested handful nonphotosynthetic proteins. To assess specificity, we quantitatively analyzed accumulation plastid two mutant lines deficient receptor....
DNA methylation is an epigenetic mechanism that has important functions in transcriptional silencing and associated with repressive histone (H3K9me). To further investigate mechanisms, we screened a mutagenized Arabidopsis thaliana population for expression of SDCpro-GFP, redundantly controlled by methyltransferases DRM2 CMT3. Here, identify the hypomorphic mutant mthfd1-1, carrying mutation (R175Q) cytoplasmic bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate...
To uncover components of the mechanism that adjusts rate leaf starch degradation to length night, we devised a screen for mutant Arabidopsis thaliana plants in which reserves are prematurely exhausted. The mutation one such mutant, named early starvation1 (esv1), eliminates previously uncharacterized protein. Starch leaves is degraded rapidly and nonlinear fashion, so exhausted 2 h prior dawn. ESV1 protein similar (named Like [LESV]) located chloroplast stroma also bound into granules....
Journal Article pep2pro: a new tool for comprehensive proteome data analysis to reveal information about organ-specific proteomes in Arabidopsis thaliana Get access Katja Baerenfaller, Baerenfaller Department of Biology, ETH Zurich, Universitaetstrasse 2, 8092 Switzerland. Fax: +41 44 6321044; Tel: 6323491 E-mail: kbaerenfaller@ethz.ch Search other works by this author on: Oxford Academic Google Scholar Matthias Hirsch-Hoffmann, Hirsch-Hoffmann Julia Svozil, Svozil Roger Hull, Hull Faculty...
Significance Members of the Arabidopsis microrchidia (AtMORC) ATPase family are involved in gene silencing and heterochromatin condensation without altering genome-wide DNA methylation patterns. Here, we examine functional relationship between several members show that AtMORC6 interacts two mutually exclusive protein complexes with AtMORC1 its closest homologue, AtMORC2. Consistently, RNA sequencing high-order mutants indicates AtMORC2 act redundantly silencing. We also genetic interactions...
Seedling establishment is a crucial phase during plant development when the germinating heterotrophic embryo switches to autotrophic growth and development. Positive regulators of embryonic need be turned off, while cell cycle machinery activated allow entry organ primordia initiation. However, it not yet understood how molecular mechanisms responsible for onset division, metabolism changes differentiation are coordinated this transition. Here, we demonstrate that Arabidopsis thaliana...
Abstract The translocon at the outer membrane of chloroplast assists import a large class preproteins with amino-terminal transit sequences. preprotein receptors Toc159 and Toc33 in Arabidopsis (Arabidopsis thaliana) are specific for accumulation abundant photosynthetic proteins. homologous GTPases known to be regulated by phosphorylation within their GTP-binding domains. In addition central domain, has an acidic N-terminal domain (A-domain) C-terminal membrane-anchoring (M-domain). A-domain...
Abstract Mediator 12 (MED12) and MED13 are components of the multi-protein complex, that facilitates initial steps gene transcription. Here, in an Arabidopsis mutant screen, we identify MED12 as positive regulators, both which contribute broadly to morc1 de-repressed expression. Both preferentially required for expression genes depleted active chromatin marks, a signature shared with re-activated loci. We further discover tends interact responsive environmental stimuli, including light...
Giardia lamblia is an intestinal protozoan parasite required to survive in the environment order be transmitted a new host. To ensure survival, flagellated trophozoites colonizing small intestine differentiate into non-motile environmentally-resistant cysts which are then shed environment. This cell differentiation process called encystation characterized by significant morphological remodeling includes secretion of large amounts cyst wall material. Although much known about transcriptional...
In Arabidopsis (Arabidopsis thaliana) leaves, starch is synthesized during the day and degraded at night to fuel growth metabolism. Starch primarily by β-amylases, liberating maltose, but this activity preceded glucan phosphorylation accompanied dephosphorylation. A phosphatase family member, LIKE SEX4 1 (LSF1), binds required for normal degradation, its exact role unclear. Here, we show that LSF1 does not dephosphorylate glucans. The recombinant dual specificity (DSP) domain of had no...
Abstract MORPHEUS’ MOLECULE1 (MOM1) is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we find that MOM1 localizes with sites RNA-directed (RdDM). Tethering artificial zinc finger unmethylated FWA promoter leads establishment and silencing. This process blocked by mutations in components the Pol V arm RdDM machinery, as well mutation MICRORCHIDIA 6 (MORC6) . We at some endogenous sites, required maintain a closed...
In flowering plants, the transition from somatic to reproductive cell fate is marked by differentiation of spore mother cells (SMCs), functional equivalent animal primordial germ (PGCs). Like their counterparts, SMCs undergo extensive structural and compositional changes in chromatin, initiated loss linker histones, a stepwise process. To investigate role mechanism H1 eviction, we focused on two candidate pathways. Our findings show that specific point mutations, affecting either putative...
The GTPases atToc33 and atToc159 are pre-protein receptor components of the translocon complex at outer chloroplast membrane in Arabidopsis. Despite their participation same vivo, evidence for interaction is still lacking. Here, a split-ubiquitin system engineered use plants, vivo Toc Arabidopsis tobacco protoplasts shown. Using method, self-interaction peroxisomal protein atPex11e demonstrated. finding suggests more general suitability as plant assay.
Isoamylase-type debranching enzymes (ISAs) play an important role in determining starch structure. Amylopectin – a branched polymer of glucose is the major component granules and its architecture underlies semi-crystalline nature starch. Mutants several species lacking ISA1-subclass isoamylase are impaired amylopectin synthesis. Consequently, levels decreased aberrant soluble glucan (phytoglycogen) with altered branch lengths branching pattern accumulates. Here we use TAP (tandem affinity...
The branched glucans glycogen and starch are the most widespread storage carbohydrates in living organisms. production of semicrystalline granules plants is more complex than that small, soluble particles microbes animals. However, factors determining whether or formed not fully understood. tropical tree Cecropia peltata a rare example an organism able to make either polymer type. Electron micrographs quantitative measurements show accumulates very high levels specialized myrmecophytic...
Abstract DNA methylation is a conserved epigenetic modification that typically associated with silencing of transposable elements and promoter methylated genes. However, some DNA-methylated loci are protected from silencing, allowing transcriptional flexibility in response to environmental developmental cues. Through genetic screen Arabidopsis (Arabidopsis thaliana), we uncovered an antagonistic relationship between the MICRORCHIDIA (MORC) protein IMITATION SWITCH (ISWI) complex regulating...
In this paper, we describe for the first time existence of organic anion transport in T lymphocytes, exemplified by transmembrane anions L-lactate and Ca2+ indicator fluo-3. The either was found to be inhibitable probenecid, a common blocker transport. Transport observed long-term cultured cell lines, as well freshly ex vivo isolated cells, occurred via saturable, pH-dependent, stereospecific process. L-Lactate uptake dependent on activation state because cells Con A strongly enhanced...