A5027042447- Plant Water Relations and Carbon Dynamics
- Tree-ring climate responses
- Tree Root and Stability Studies
- Plant Diversity and Evolution
- Plant Pathogens and Fungal Diseases
- Forest ecology and management
- African Botany and Ecology Studies
- Advanced Cellulose Research Studies
- Lignin and Wood Chemistry
- Wood and Agarwood Research
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Enzyme-mediated dye degradation
- Botanical Research and Applications
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Nanocomposite Films for Food Packaging
- Plant Molecular Biology Research
- Plant Surface Properties and Treatments
- Bamboo properties and applications
- Cultural Heritage Materials Analysis
- Postharvest Quality and Shelf Life Management
- Natural Fiber Reinforced Composites
- Remote Sensing and LiDAR Applications
Forest Products Laboratory
2012-2025
University of Washington
2020-2023
Nanjing Forestry University
2023
Royal Museum for Central Africa
2009-2022
US Forest Service
2016-2022
Tokyo University of Agriculture and Technology
2020-2022
University of Wisconsin–Madison
2012-2019
Nanyang Technological University
2019
Madison Group (United States)
2016
Madison Area Technical College
2015-2016
Concentrated solid dicarboxylic acid hydrolysis for producing highly thermal-stable and carboxylated cellulose nanomaterials with complete recovery.
Abstract Transgenic down-regulation of the Pt4CL1 gene family encoding 4-coumarate:coenzyme A ligase (4CL) has been reported as a means for reducing lignin content in cell walls and increasing overall growth rates, thereby improving feedstock quality paper bioethanol production. Using hybrid poplar (Populus tremula × Populus alba), we applied this strategy examined field-grown transformants both effects on wood biochemistry tree productivity. The reductions contents obtained correlated well...
ABSTRACT We studied xylem anatomy and hydraulic architecture in 14 transgenic insertion events a control line of hybrid poplar ( Populus spp.) that varied lignin content. Transgenic had different levels down‐regulation two genes encoding 4‐coumarate:coenzyme A ligase (4CL). Two‐year‐old trees were characterized after growing either as free‐standing the field or supported by stakes greenhouse. In trees, 20 to 40% reduction content was associated with increased vulnerability embolism, shoot...
This paper reviews the development of xylem vessels in ring-porous dicots and corresponding leaf phenology. Also included are our original observations on time-course vessel element growth, secondary wall deposition, end perforation deciduous hardwood Kalopanax septemlobus . Different patterns growth phenology serve different strategies species for adaptation to seasonal climates. Trees with form wide earlywood (EWV) spring narrow latewood summer. The EWV become embolized or blocked tyloses...
Abstract Of 14 transgenic poplar genotypes (Populus tremula × Populus alba) with antisense 4-coumarate:coenzyme A ligase that were grown in the field for 2 years, five had substantial lignin reductions also greatly reduced xylem-specific conductivity compared of control trees and those events small lignin. For two lowest xylem contents (greater than 40% reduction), we used light microscopy methods acid fuchsin dye ascent studies to clarify what caused their transport efficiency. novel...
Abstract Investigating plant structure is fundamental in botanical science and provides crucial knowledge for the theories of evolution, ecophysiology biotechnological practices. Modern anatomy often targets formation, localization characterization cellulosic, lignified or suberized cell walls. While classical methods developed 1960s are still popular, recent innovations tissue preparation, fluorescence staining microscopy equipment offer advantages to traditional practices investigation...
Teak forms xylem rings that potentially carry records of carbon sequestration and climate in the tropics. These are only useful when structural variations tree their periodicity formation known. The seasonality ring mature teak trees was examined via correlative analysis cambial activity, phloem formation, throughout 1·5 years. Xylem differentiation were visualized by light microscopy scanning electron microscopy. A 3 month dry season resulted semi-deciduousness, dormancy annual growth...
Since uncertainty remains about how white rot fungi oxidize and degrade lignin in wood, it would be useful to monitor changes fungal gene expression during the onset of ligninolysis on a natural substrate. We grew Phanerochaete chrysosporium solid spruce wood included oxidant-sensing beads bearing fluorometric dye BODIPY 581/591 cultures. Confocal fluorescence microscopy showed that extracellular oxidation commenced 2 3 days after inoculation, coincident with cessation growth. Whole...
Xylogenesis is synchronous among trees in regions with a distinct growing season, leading to forest-wide time lag between growth and carbon uptake. In contrast, little known about interspecific or even intraspecific variability of xylogenesis tropical forests. Yet an understanding patterns key successfully combine bottom-up (e.g., from permanent forest inventory plots) top-down eddy covariance flux towers) estimates. Here, we monitor development 18 belonging 6 abundant species during 8 weeks...
Abstract There is an increasing need for inexpensive biodegradable sensors that can be easily employed in networks such as the Internet of Things. Paper materials are renewable, biodegradable, and sustainable, thus could used substrates electronic sensors. This work examined two commodity cellulose materials, envelope paper a linerboard, potential substrates. A multistage coating process was developed to create smooth surface screen-printing using microfibrillated cellulose. Employing this...
The three‐dimensional (3‐D) arrangement of vessels and the vessel‐to‐vessel connections in secondary xylem stem ring‐porous hardwood tree Fraxinus lanuginosa were studied series thick transverse sections with epifluorescence microscope confocal laser scanning microscope. Vessels traced sequential sections, vessel networks reconstructed two segments wood dimensions 2 × 1.4 21.2 mm 3 5.8 (tangential radial axial). intervessel pits visualized by electron microscopy low‐density polyethylene...
Background and AimsAlthough the lateral movement of water gas in tree stems is an important issue for understanding physiology, as well development wood preservation technologies, little known about vascular pathways radial flow. The aim current study was to understand occurrence structure anatomical features sugi (Cryptomeria japonica) including tracheid networks, area fractions intertracheary pits, tangential walls ray cells intercellular spaces that may be related permeability...
• Premise of the study: The pathway radial water movement in tree stems presents an unknown with respect to whole‐tree hydraulics. Radial profiles have shown substantial axial sap flow deeper layers sapwood (that may lack direct connection transpiring leaves), which suggests existence a for movement. Rays include ray tracheids and/or parenchyma cells and offer such transport. This study investigated relationships between hydraulic conductivity ( k s‐rad ) anatomical stem morphological...
Samples of a mature specimen Kalopanax pictus , ring-porous hardwood, were studied to compare the respective lengths fusiform cambial cells and vessel elements in stem. The dormant reactivated measured with confocal laser scanning microscope tissue that had been macerated by digestion pectinase thick tangential sections. early wood late tissues Franklin's method. varied considerably length within individual samples. mean corresponded cambium but not cambium. Significant differences observed...
Summary Polyhydroxybutyrate (PHB) is a bioplastic that can be produced in transgenic plants by the coexpression of three bacterial genes for its biosynthesis. PHB yields from have been constrained negative impacts on plant health result diversion resources into production; thus, we employed an ecdysone analogue–based system induced gene expression. We characterized 49 insertion events hybrid poplar ( Populus tremula x alba ) were using Agrobacterium transformation and studied two...
Charcoal was sampled in four soil profiles at the Mayumbe forest boundary (DRC). Five fire events were recorded and 44 charcoal types identified. One stratified profile yielded assemblages around 530 cal yr BP > 43.5 ka age. The oldest assemblage precedes period of anthropogenic burning, illustrating occasional long-term absence but also natural wildfire occurrences within tropical rainforest. No other older than 2500 recorded, perhaps due to bioturbation colluvial reworking. paleofires...
Colonization of wood blocks by brown and white rot fungi rapidly resulted in detectable oxidation, as shown a reduced phloroglucinol response, loss autofluorescence, acridine orange (AO) staining. This last approach is to provide novel method for identifying oxidation. When lignin was mildly oxidized, the association between AO such that stained sections emitted less green light during fluorescence microscopy. change after than week, an interval past work has be too short significant...