A5077983192- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Biochemical and biochemical processes
- Chromium effects and bioremediation
- Fungal and yeast genetics research
- Mycorrhizal Fungi and Plant Interactions
- Aluminum toxicity and tolerance in plants and animals
- Trace Elements in Health
- Plant Micronutrient Interactions and Effects
- Diatoms and Algae Research
- Metal Extraction and Bioleaching
- Microbial Metabolism and Applications
- Plant tissue culture and regeneration
- Slime Mold and Myxomycetes Research
- Plant Genetic and Mutation Studies
- Multicomponent Synthesis of Heterocycles
- Synthesis and biological activity
- Enzyme Production and Characterization
- Medicinal Plants and Neuroprotection
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer-related molecular mechanisms research
- Biofuel production and bioconversion
- Parathyroid Disorders and Treatments
- RNA modifications and cancer
- Magnesium in Health and Disease
Mahatma Gandhi University
2013-2023
Forest Products Laboratory
2011-2012
University of Wisconsin–Madison
2011-2012
Osmania University
2008-2011
Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major failure wooden structures. Recent work indicates distinct lineages fungi have arisen independently from ligninolytic white ancestors via loss lignocellulolytic enzymes. Brown thus proceeds without significant lignin removal, apparently beginning instead with oxidative attack on polymers by Fenton reagent produced when fungal hydroquinones or catechols reduce Fe(3+)...
Since uncertainty remains about how white rot fungi oxidize and degrade lignin in wood, it would be useful to monitor changes fungal gene expression during the onset of ligninolysis on a natural substrate. We grew Phanerochaete chrysosporium solid spruce wood included oxidant-sensing beads bearing fluorometric dye BODIPY 581/591 cultures. Confocal fluorescence microscopy showed that extracellular oxidation commenced 2 3 days after inoculation, coincident with cessation growth. Whole...
ABSTRACT Brown rot basidiomycetes have an important ecological role in lignocellulose recycling and are notable for their rapid degradation of wood polymers via oxidative hydrolytic mechanisms. However, most these fungi apparently lack processive ( exo -acting) cellulases, such as cellobiohydrolases, which generally required efficient cellulolysis. The recent sequencing the Postia placenta genome now permits a proteomic approach to this longstanding conundrum. We grew P. on solid aspen wood,...
ABSTRACT We report here the identification and characterization of mrdH , a novel chromosomal metal resistance determinant, located in genomic island 55 Pseudomonas putida KT2440. It encodes for MrdH, predicted protein ∼40 kDa with chimeric domain organization derived from RcnA RND (for resistance-nodulation-cell division) efflux proteins. The function was identified by ability to confer nickel upon its complementation into rcnA mutant (a nickel- cobalt-sensitive mutant) Escherichia coli ....
Oxidative cleavage of the recalcitrant plant polymer lignin is a crucial step in global carbon cycling, and accomplished most efficiently by fungi that cause white rot wood. These basidiomycetes secrete many enzymes metabolites with proposed ligninolytic roles, it not clear whether all these agents are physiologically important during attack on natural lignocellulosic substrates. One new approach to this problem infer properties oxidants from their spatial distribution relative fungus...
Nine actinobacterial isolates were purified from the sediment sample of Kogilvai village, Warangal, Telangana, based on their capability to grow minimal medium with Ferulic acid (FA) as only Carbon (C) source. FA Vanillin conversion capacity these was identified by Thin-layer chromatography (TLC). Biotransformation high four isolates, S1, S3, O3 and O4 when compared other five (O1, O2, S2, S4 S5) initial pH 7 in basal medium. Among optimal rapid bioconversion 140 mg/L shown isolate S1...
The Neurospora crassa genome encodes four putative CorA transporters Tmg-1, Tmg-2, Tmg-3, and Tmg-4. GQN motif is found on the Tmg-4 transporter encoded by NCU07816.5 (tmg-4) gene in contrast to conserved GMN which essential for structure function of magnesium transporters. We were intrigued see wide distribution variant place across fungi. Using various bioinformatics tools, we catalogued around 300 protein sequences with tentative superfamily features from 100 fungal species belonging all...
To unveil the physiological relevance of magnesium and its transport pathways in Neurospora crassa, vegetative, asexual, sexual phases development were investigated. Notably, a regular rate hyphal growth extension was observed media without supplementation. Further, conidia perithecia formation completely abolished under same conditions. By estimating levels mycelial cations, identified as 3rd most abundant ion found to be mediated by four putative CorA transporters: Tmg-1, Tmg-2, Tmg-3,...
In an attempt to synthesize new chemical entities with promising biological activity, a set of six novel pyrimidinedione analogues 6(a-f) was prepared. The compound 4 coupled different amines by using HBTU as coupling reagent. derivatives were characterized 1HNMR,13CNMR and HRMS. evaluated for their antibacterial antioxidant activity. DPPH ABTS methods used test the activity moieties. Among analogues, 6b, 6d, 6f exhibited significant effects. paper disc diffusion method adopted evaluate...