A5062198223- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant and Biological Electrophysiology Studies
- Plant Reproductive Biology
- Sphingolipid Metabolism and Signaling
- Photosynthetic Processes and Mechanisms
- Antimicrobial Peptides and Activities
- Ion channel regulation and function
- Plant tissue culture and regeneration
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Botanical Research and Applications
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- 14-3-3 protein interactions
- Calcium signaling and nucleotide metabolism
- Ubiquitin and proteasome pathways
- bioluminescence and chemiluminescence research
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- Botanical Research and Chemistry
- Ginseng Biological Effects and Applications
- Nuclear Structure and Function
- Modular Robots and Swarm Intelligence
- Erythrocyte Function and Pathophysiology
Laboratoire de Recherche en Sciences Végétales
2011-2024
Centre National de la Recherche Scientifique
1998-2023
Institut National Polytechnique de Toulouse
2023
Université Toulouse III - Paul Sabatier
1994-2016
Université de Toulouse
2011-2016
University of California, San Diego
1991-1994
ABSTRACT Cadmium is suspected to exert its toxic action on cells through oxidative damage. However, the transition metal unable directly generate reactive oxygen species (ROS) via redox reactions with molecular in a biological environment. Here, we show that bright yellow‐2 (BY‐2) tobacco exposed millimolar concentrations of CdCl 2 developed cell death within 2–3 h. The process was preceded by two successive waves ROS differing their nature and subcellular localization. Firstly, these...
MicroRNAs (miRNAs) are transcribed as long primary transcripts (pri-miRNAs) by RNA polymerase II. Plant pri-miRNAs encode regulatory peptides called miPEPs, which specifically enhance the transcription of pri-miRNA from they originate. However, paradoxically, whereas miPEPs have been identified in different plant species, poorly conserved, raising question mechanisms underlying their specificity. To address this point, we identify and re-annotate multiple Arabidopsis thaliana order to ORF...
Summary Depolarization‐activated plasma membrane calcium channels have been suggested to play prominent roles in signal perception and transduction processes during growth development of higher plants. The existence such has recently established plant cells. However, patch–clamp experiments shown that their activity is very low decreases rapidly after the establishment whole‐cell configuration, due most probably protein–protein interactions involving microtubules. present study takes...
Abstract The current agriculture main challenge is to maintain food production while facing multiple threats such as increasing world population, temperature increase, lack of agrochemicals due health issues and uprising weeds resistant herbicides. Developing novel, alternative, safe methods hence paramount importance. Here, we show that complementary peptides (cPEPs) from any gene can be designed target specifically plant coding genes. External application synthetic increases the abundance...
Ca2+ channels have been suggested to play major roles in the initiation of a large number signal transduction processes higher plant cells, including bud formation, polar growth, gas exchange regulation, secretion, move? ments, and light-regulated hormone-regulated growth development (Hepler Wayne, 1985; Leonard Hepler, 1990). Activation allows transmembrane fluxes Ca2+. These result elevation cytosolic concentration from resting levels approximately 150 nM excitatory greater than 300 400...
MicroRNAs (miRNAs) are small regulatory RNA molecules (21–24 nt) regulating the expression of target genes at post-transcriptional level, by cleaving their mRNA. Because these mainly genes, miRNAs involved crossroads several biological processes (Liu et al., 2018). transcribed as long primary transcripts (pri-miRNAs) (Xie 2005). Recent findings revealed that plant pri-miRNAs encode peptides called miRNA-encoded (miPEPs) (Lauressergues 2015; Sharma 2020). miPEPs specifically enhance...
Plasma membrane‐bound voltage‐dependent calcium channels may couple the perception of an initial stimulus to a regulated pathway for influx. The activities these have been shown be very low and highly unstable but recruited by large‐predepolarizing pulses, according process referred as recruitment. By combining pharmacological electrophysiological approaches, we demonstrate in present paper that cytoskeleton plays important role regulation activity stability during whole‐cell patch‐clamp...
MiPEPs are short natural peptides encoded by microRNAs in plants. Exogenous application of miPEPs increases the expression their corresponding miRNA and, consequently, induces consistent phenotypical changes. Therefore, carry huge potential agronomy as gene regulators that do not require genome manipulation. However, to this end, it is necessary know mode action, including where they act and how enter Here, after analyzing effect Arabidopsis thaliana miPEP165a on root aerial part...
Sphinganine or dihydrosphingosine (d18:0, DHS), one of the most abundant free sphingoid long chain bases (LCBs) in plants, is known to induce a calcium-dependent programmed cell death (PCD) plants. In addition, tobacco BY-2 cells, it has been shown that DHS triggers rapid production H2O2 and nitric oxide (NO). Recently, analogy what animal field, plant cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), ubiquitous enzyme involved glycolysis, suggested fulfill other functions...
The biggest challenge for global agriculture today is to combat pathogens, especially fungi, with fewer and active molecules available. In fact, fungi are responsible 10%–20% of crop losses, equivalent a loss up $200 billion per year (www.ars.usda.gov). Moreover, the use current fungicides (mainly chemicals) often associated health environmental issues (Fungicide_Uses_and_Risks). Finally, becoming increasingly resistant currently used (Fungicide_Resistance_Management). this context,...
Calcium channels have been suggested to play a major role in the initiation of large number signal transduction processes higher plant cells. However, molecular components Ca2+ remain unidentified date. channel blockers phenylalkylamine family and bepridil specifically inhibit influx into carrot (Daucus carota L.) By using azido derivative, 75-kDa membrane protein has previously identified. Here we partially purified this blocker-binding by lectin-affinity ion-exchange chromatographies. The...