A5050912648- Plant Pathogens and Resistance
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Plant Reproductive Biology
- Powdery Mildew Fungal Diseases
- Plant pathogens and resistance mechanisms
- Genetic and Environmental Crop Studies
- Aquaculture disease management and microbiota
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
- Mycorrhizal Fungi and Plant Interactions
- Genomics and Phylogenetic Studies
- Photosynthetic Processes and Mechanisms
- Agriculture, Plant Science, Crop Management
- Agronomic Practices and Intercropping Systems
- Plant tissue culture and regeneration
- Invertebrate Immune Response Mechanisms
- Infectious Diseases and Mycology
Laboratoire de Recherche en Sciences Végétales
2015-2025
Centre National de la Recherche Scientifique
2012-2025
Université Toulouse III - Paul Sabatier
2011-2025
Université de Toulouse
2010-2025
Centre National pour la Recherche Scientifique et Technique (CNRST)
2003-2025
AVL (France)
2007
Many oomycete and fungal plant pathogens are obligate biotrophs, which extract nutrients only from living tissue cannot grow apart their hosts. Although these cause substantial crop losses, little is known about the molecular basis or evolution of biotrophy. Here, we report genome sequence Hyaloperonospora arabidopsidis (Hpa), an biotroph natural pathogen Arabidopsis thaliana. In comparison with genomes related, hemibiotrophic Phytophthora species, Hpa exhibits dramatic reductions in genes...
Abstract Background Pythium ultimum is a ubiquitous oomycete plant pathogen responsible for variety of diseases on broad range crop and ornamental species. Results The P. genome (42.8 Mb) encodes 15,290 genes has extensive sequence similarity synteny with related Phytophthora species, including the potato blight infestans . Whole transcriptome sequencing revealed expression 86% genes, detectable differential suites under abiotic stress in presence host. predicted proteome includes large...
Pathogens use specialized secretion systems and targeting signals to translocate effector proteins inside host cells, a process that is essential for promoting disease parasitism. However, the amino acid sequences determine delivery of eukaryotic pathogen effectors remain mostly unknown. The Crinkler (CRN) oomycete plant pathogens, such as Irish potato famine organism Phytophthora infestans, are modular with predicted conserved N-terminal sequence motifs. Here, we provide direct evidence CRN...
Oomycetes in the class Saprolegniomycetidae of Eukaryotic kingdom Stramenopila have evolved as severe pathogens amphibians, crustaceans, fish and insects, resulting major losses aquaculture damage to aquatic ecosystems. We sequenced 63 Mb genome fresh water pathogen, Saprolegnia parasitica. Approximately 1/3 assembled exhibits loss heterozygosity, indicating an efficient mechanism for revealing new variation. Comparison S. parasitica with plant pathogenic oomycetes suggests that during...
The cellulose binding elicitor lectin (CBEL) from Phytophthora parasitica nicotianae contains two domains (CBDs) belonging to the Carbohydrate Binding Module1 family, which is found almost exclusively in fungi. mechanism by CBEL perceived host plant remains unknown. role of CBDs eliciting activity was investigated using modified versions protein produced Escherichia coli or synthesized planta through potato virus X expression system. Recombinant E. elicited necrotic lesions and defense gene...
SUMMARY The Oomycete genus Aphanomyces houses plant and animal pathogens found in both terrestrial aquatic habitats . euteiches Drechs. causes seedling damping off root rot diseases on many legumes. It is the most devastating pea ( Pisum sativum ) disease several countries, causing up to 80% losses each year. This strictly soil‐borne pathogen may survive years soil no efficient chemical control currently available. only way avoid cultivating legumes infested fields for 10 years. Although...
The cell wall of the oomycete plant pathogen Phytophthora parasitica var. nicotianae contains a protein called CBEL that shows cellulose-binding (CB), elicitor (E) defense in plants and lectin-like (L) activities. biological role this molecule was investigated by generating transgenic strains suppressed expression. Phenotypic characterization these showed they were severely impaired adhesion to cellophane membrane, differentiation lobed structures contact with cellophane, formation branched...
Aphanomyces euteiches is an oomycete pathogen that causes seedling blight and root rot of legumes, such as alfalfa pea. The genus phylogenically distinct from well-studied oomycetes Phytophthora sp., contains species pathogenic on plants aquatic animals. To provide the first foray into gene diversity A. euteiches, two cDNA libraries were constructed using mRNA extracted mycelium grown in artificial liquid medium or contact to plant roots. A unigene set 7,977 sequences was obtained 18,864...
Oomycetes are a group of filamentous eukaryotic microorganisms that have colonized all terrestrial and oceanic ecosystems, they include prominent plant pathogens. The Aphanomyces genus is unique in its ability to infect both animal species, as such exemplifies oomycete versatility adapting different hosts environments. Dissecting the underpinnings diversity provides insights into their specificity pathogenic mechanisms. By carrying out genomic analyses pathogen A. euteiches crustacean...
The cellulose binding elicitor lectin (CBEL) of the genus Phytophthora induces necrosis and immune responses in several plant species, including Arabidopsis thaliana. However, role CBEL-induced outcome interaction is still unclear. This study shows that some defence responses, but not necrosis, required receptor-like kinase BAK1, a general regulator basal immunity Arabidopsis, production reactive oxygen burst mediated by respiratory oxidases homologues (RBOH). Screening core collection 48...
Abstract Background Oomycetes are fungal-like microorganisms evolutionary distinct from true fungi, belonging to the Stramenopile lineage and comprising major plant pathogens. Both oomycetes fungi express proteins able interact with cellulose, a component of oomycete cell walls, through presence carbohydrate-binding module family 1 (CBM1). Fungal CBM1-containing were implicated in cellulose degradation whereas oomycetes, Cellulose Binding Elicitor Lectin (CBEL), well-characterized...
Summary To successfully colonize their host, pathogens produce effectors that can interfere with host cellular processes. Here we investigated the function of CRN 13 candidate produced by plant pathogenic oomycetes and detected in genome amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd 13). When expressed Nicotiana , Ae 13, from legume root pathogen Aphanomyces euteiches increases susceptibility leaves to oomycete Phytophthora capsici . transiently amphibians or cells, Bd localize...
PEG-mediated transformation of protoplasts in the presence lipofectin was achieved Phytophthora parasitica var. nicotianae, an oomycete pathogen tobacco. Using promoter and terminator sequences, a plant-adapted green fluorescent protein (GFP) introduced into microorganism. The data show for first time that this eukaryotic gene reporter can be used oomycete, both as quantitative induction vital marker allowing study development vitro host plant.
• Sterols are isoprenoid-derived molecules that have essential functions in eukaryotes but whose metabolism remains largely unknown a large number of organisms. Oomycetes fungus-like microorganisms evolutionarily related to stramenopile algae, group organisms for which no sterol metabolic pathway has been reported. Here, we present data support model biosynthesis Aphanomyces euteiches, an oomycete species causing devastating diseases legume crops. In silico analyses were performed identify...
Root diseases are a major global threat to sustainable agricultural production. Complexes of interacting soilborne fungal, oomycete and nematode pathogens involved in causing these that generally termed root disease complexes. These pathogen complexes typically have resilient long-term survival mechanisms, the ability cross-infect multiple crop genera species, wide range spatial dispersal modes. Although, some instances, individual within complex been identified using classical molecular...
CLPG1, an endopolygalacturonase (endoPG) gene of Colletotrichum lindemuthianum, was transferred to tobacco (Nicotiana tabacum) leaves by using the Agrobacterium tumefaciens transient delivery system. The following four constructs were prepared: with or without its signal peptide (SP; PG1, PG1deltaSP); CLPG1 expansin1 SP instead own (Exp::PG1deltaSP); and a mutated version latter on two amino acids potentially involved in catalytic site (D202N/D203N). Chlorotic necrotic lesions appeared 5 7 d...
Abstract Background The Oomycete genus Aphanomyces comprises devastating plant and animal pathogens. However, little is known about the molecular mechanisms underlying pathogenicity of species. In this study, we report on development a public database called AphanoDB which dedicated to genomic data. As first step, large collection Expressed Sequence Tags was obtained from legume pathogen A. euteiches , then processed collected into AphanoDB. Description Two cDNA libraries were created: one...
Oomycete plant pathogens secrete effector proteins to promote disease. The damaging soilborne legume pathogen Aphanomyces euteiches harbors a specific repertoire of Small Secreted Protein effectors (AeSSPs), but their biological functions remain unknown. Here we characterize AeSSP1256. function AeSSP1256 is investigated by physiological and molecular characterization Medicago truncatula roots expressing the effector. A potential protein target identified yeast-two hybrid,...
Pythium oligandrum is a soil-borne oomycete associated with rhizosphere and root tissues. Its ability to enhance plant growth, stimulate immunity parasitize fungal preys has led the development of agricultural biocontrol products. Meanwhile, effect P. on mutualistic interactions more generally microbial communities not been investigated. Here, we developed biological system comprising interacting two legume plants, Medicago truncatula Pisum sativum. activity was investigated at...
Pythium oligandrum is a soil born free living oomycete able to parasitize fungi and oomycetes prey, including important plant animals pathogens. can colonize endophytically the root tissues of diverse plants where it induces defenses. Here we report first long-read genome sequencing P. strain sequenced by PacBio technology. Sequencing genomic DNA loaded onto six SMRT cells permitted acquisition 913,728 total reads resulting in 112X coverage. The assembly polishing sequence yielded180 contigs...