A5009613995- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Essential Oils and Antimicrobial Activity
- Fungal Biology and Applications
- Mycotoxins in Agriculture and Food
- Wheat and Barley Genetics and Pathology
- Insect Resistance and Genetics
- Mycorrhizal Fungi and Plant Interactions
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Leptospirosis research and findings
- Plant and Fungal Interactions Research
- Plant tissue culture and regeneration
- Toxin Mechanisms and Immunotoxins
- Insect Pest Control Strategies
- Fungal Plant Pathogen Control
- Vibrio bacteria research studies
- Beetle Biology and Toxicology Studies
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Evolution and Genetic Dynamics
- Medicinal plant effects and applications
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Biologie et Gestion des Risques en Agriculture
2015-2024
Université Paris-Saclay
2017-2024
Sainsbury Laboratory
2019-2024
University of East Anglia
2019-2024
Norwich Research Park
2019-2024
AgroParisTech
2019-2021
University of Bonn
2021
Syngenta (Switzerland)
2021
Institut National de la Recherche Agronomique du Niger
2019
Recognition of a pathogen avirulence (AVR) effector protein by cognate plant resistance (R) triggers set immune responses that render the resistant. Pathogens can escape this so-called Effector-Triggered Immunity (ETI) different mechanisms including deletion or loss-of-function mutation AVR gene, incorporation point mutations allow recognition to be evaded while maintaining virulence function, and acquisition new effectors suppress recognition. The Dothideomycete Leptosphaeria maculans,...
Bioengineering of plant immune receptors has emerged as a key strategy for generating novel disease resistance traits to counteract the expanding threat pathogens global food security. However, current approaches are limited by rapid evolution in field and may lack durability when deployed. Here, we show that rice nucleotide-binding, leucine-rich repeat (NLR) receptor Pik-1 can be engineered respond conserved family effectors from multihost blast fungus pathogen Magnaporthe oryzae . We...
Summary Ustilago maydis is the causal agent of maize smut disease. During colonization process, fungus secretes effector proteins that suppress immune responses and redirect host metabolism in favor pathogen. As effectors play a critical role during plant colonization, their identification functional characterization are essential to understanding biotrophy Using biochemical, molecular, transcriptomic techniques, we performed U. Jasmonate/Ethylene signaling inducer 1 (Jsi1). Jsi1 interacts...
Accelerated gene evolution is a hallmark of pathogen adaptation and specialization following host-jumps. However, the molecular processes associated with adaptive between host-specific lineages multihost plant remain poorly understood. In blast fungus Magnaporthe oryzae (Syn. Pyricularia ), host on different grass hosts generally dynamic patterns gain loss virulence effector genes that tend to define distinct genetic this pathogen. Here, we unravelled biochemical structural basis APikL2, an...
Abstract Bioengineering of plant immune receptors has emerged as a key strategy for generating novel disease resistance traits to counteract the expanding threat pathogens global food security. However, current approaches are limited by rapid evolution in field and may lack durability when deployed. Here, we show that rice nucleotide-binding, leucine-rich repeat (NLR) receptor Pik-1 can be engineered respond conserved family effectors from multihost blast fungus pathogen Magnaporthe oryzae ....
Interactions between Leptosphaeria maculans, causal agent of stem canker oilseed rape, and its Brassica hosts are models choice to explore the multiplicity 'gene-for-gene' complementarities how they diversified increased complexity in course plant-pathogen co-evolution. Here, we support this postulate by investigating AvrLm10 avirulence that induces a resistance response when recognized nigra gene Rlm10. Using genome-assisted map-based cloning, identified cloned two candidates as genes...
The blast fungus Magnaporthe oryzae (syn. Pyricularia oryzae) is a destructive plant pathogen that can infect about 50 species of both wild and cultivated grasses, including important crops such as rice wheat. M. composed genetically differentiated lineages tend to specific host genera. To date, most studies effectors have focused on the rice-infecting lineage. We describe clone resource 195 predicted from all major host-specific lineages. These clones are freely available Golden...
Abstract Fungal effectors (small‐secreted proteins) have long been considered as species or even subpopulation‐specific. The increasing availability of high‐quality fungal genomes and annotations has allowed the identification trans‐species trans‐genera families effectors. Two avirulence effectors, AvrLm10A AvrLm10B , Leptosphaeria maculans fungus causing stem canker oilseed rape, are members such a large family neighbouring genes, organized in divergent transcriptional orientation. Sequence...
Abstract Despite large omics datasets, the establishment of a reliable gene annotation is still challenging for eukaryotic genomes. Here, we used reference genome major fungal wheat pathogen Zymoseptoria tritici (isolate IPO323) as case study to develop methods improve prediction. Four previous IPO323 annotations identified 10,933 13,260 models, but only one third these coding sequences (CDS) have identical structures. To resolve discrepancies and generated full-length transcripts using...
Abstract Recognition of a pathogen avirulence (AVR) effector protein by cognate plant resistance (R) triggers set immune responses that render the resistant. Pathogens can escape this so-called Effector-Triggered Immunity (ETI) different mechanisms including deletion or loss-of-function mutation AVR gene, incorporation point mutations allow recognition to be evaded while maintaining virulence function, and acquisition new effectors suppress recognition. The Dothideomycete Leptosphaeria...
Abstract Fungal effectors play crucial roles in plant infection. Despite low sequence identity, were recently classified into structural families. In this study, we have elucidated the structures of Zt-NIP1 and Mycgr3-91409 wheat fungal pathogen Zymoseptoria tritici, using X-ray crystallography NMR. These displayed a homology with, respectively, KP4 KP6α killer toxins, from UmV dsRNA viruses infecting corn Ustilago maydis . Consequently, renamed Zt-KP4-1 Zt-KP6-1. Orthologs paralogs Zt-KP6-1...
Abstract Accelerated gene evolution is a hallmark of pathogen adaptation and specialization following host-jumps. However, the molecular processes associated with adaptive between host-specific lineages multihost plant remain poorly understood. In blast fungus Magnaporthe oryzae (Syn. Pyricularia oryzae), host on different grass hosts generally dynamic patterns gain loss virulence effector genes that tend to define distinct genetic this pathogen. Here, we unravelled biochemical structural...
Abstract Ustilago maydis (U. maydis) is the causal agent of maize smut disease. During colonization process, fungus secretes effector proteins which suppress immune responses and redirect host-metabolism in favor pathogen. Here we describe a novel strategy by U. induces plant jasmonate/ethylene (JA/ET) hormone signaling thereby biotrophic susceptibility. The Jasmonate/Ethylene inducer 1 ( Jsi1 ) possesses an ethylene-responsive element binding factor-associated amphiphilic repression (EAR)...
Abstract With only a few exceptions, fungal effectors (small secreted proteins) have long been considered as species- or even isolate-specific. the increasing availability of high-quality genomes and annotations, trans-species trans-genera families are being uncovered. Two avirulence effectors, AvrLm10A AvrLm10B , Leptosphaeria maculans fungus responsible for stem canker oilseed rape, members such large family effectors. neighboring genes, organized in divergent transcriptional orientation....
No matter how long the journey, arriving at Asilomar conference grounds for Fungal Genetics Conference is like walking into an oasis – both ecologically and scientifically. Here, unending views of Pacific sound ocean together with energizing interaction between peers combined to make yet another memorable (Fig. 1). Keeping time-honoured tradition, this year's organisers brought visionaries in field fungal genetics discuss latest our understanding genomic mechanistic underpinnings symbiosis,...