A5053726753- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Nutrition and Health in Aging
- Antioxidant Activity and Oxidative Stress
- Atherosclerosis and Cardiovascular Diseases
- Lipid metabolism and biosynthesis
- Diet and metabolism studies
- T-cell and B-cell Immunology
- Frailty in Older Adults
- Immune Cell Function and Interaction
- Metabolism and Genetic Disorders
- Dementia and Cognitive Impairment Research
- GDF15 and Related Biomarkers
- Fatty Acid Research and Health
- Renal function and acid-base balance
- Health Systems, Economic Evaluations, Quality of Life
- CAR-T cell therapy research
- Sphingolipid Metabolism and Signaling
- Vitamin C and Antioxidants Research
- Nutritional Studies and Diet
- Clinical Nutrition and Gastroenterology
- Regulation of Appetite and Obesity
- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cholesterol and Lipid Metabolism
Université Toulouse III - Paul Sabatier
2015-2024
Centre Hospitalier Universitaire de Toulouse
2001-2024
Inserm
2014-2024
Institut des Maladies Métaboliques et Cardiovasculaires
2014-2024
Université de Toulouse
2009-2024
Laboratoire de Biochimie
2006-2023
Médipole Garonne
2022
French Clinical Research Infrastructure Network
2021
Hôpital Larrey
2021
Centre d’Investigation Clinique 1436
2020
Close relationships have been demonstrated between adipose tissue and the inflammatory/immune system. Furthermore, obesity is increasingly considered as a state of chronic inflammation. Cytofluorometric analysis reveals presence significant levels lymphocytes in stroma‐vascular fraction white tissues. In epididymal (EPI) fat, display an “ancestral” immune system phenotype (up to 70% natural killer (NK), γδ + T NKT cells among all lymphocytes) whereas inguinal (ING) presents more adaptive...
Partial inhibition of adipose tissue lipolysis does not increase fat mass but improves glucose metabolism and insulin sensitivity through modulation fatty acid turnover induction cell de novo lipogenesis.
We hypothesized that skeletal muscle contraction produces a cellular stress signal, triggering adipose tissue lipolysis to sustain fuel availability during exercise. The present study aimed at identifying exercise-regulated myokines, also known as exerkines, able promote lipolysis. Human primary myotubes from lean healthy volunteers were submitted electrical pulse stimulation (EPS) mimic either acute intense or chronic moderate Conditioned media (CM) experiments with human adipocytes...
TNF-alpha is a pleiotropic cytokine activating several signaling pathways initiated at distinct intracellular domains of the TNF receptors. Although C-terminal region believed to be responsible for apoptosis induction, functions more membrane-proximal domains, including domain that couples neutral sphingomyelinase activation, are not yet fully elucidated. The roles this and associated adapter protein FAN (factor with SMase activation) in cytotoxic response have been investigated. We now...
An efficient regiospecific total synthesis of several branched fatty acyl hydroxyl-fatty acids (FAHFA) has been achieved from available terminal alkenes and alkynes. The key steps feature a boron trifluoride mediated epoxide ring opening with acetylide carbanions, followed by hydrogenation the alkyne function. carboxylic acid hydroxylated chains is introduced at last step to allow esterification hydroxyl group beforehand. chemical syntheses "linear" FAHFA analog containing Z-olefin in chain...
Nutrigenomics investigates relationships between nutrients and all genome-encoded molecular entities. This holistic approach requires systems biology to scrutinize the effects of diet on tissue biology. To decipher adipose (AT) response induced weight changes we focused key (lipids transcripts) AT species during a longitudinal dietary intervention. obtain model, network was used combine sets variables (bio-clinical, fatty acids mRNA levels) get an overview their interactions. levels were...
ABSTRACT Whole-body energy expenditure, as well glucose and lipid metabolism, are regulated by skeletal muscles, which account for 40-50% of human body mass. Peroxisomes dynamic organelles that play a crucial role in metabolism clearance reactive oxygen species, however their muscles remains poorly understood. To clarify this issue, we generated muscle-specific transgenic mouse line with peroxisome import deficiency resulting from deletion peroxisomal biogenesis factor 5 (Pex5). Pex5...
Oxidized low density lipoproteins (oxLDL) are thought to play a central role in the development of atherosclerosis. Toxic concentrations mildly oxidized LDL elicit massive apoptosis endothelial cells (Escargueil-Blanc, I., Meilhac, O., Pieraggi, M. T., Arnal J. F., Salvayre, R., Nègre-Salvayre, A. (1997)Arterioscler. Thromb. Vasc.Biol. 17, 331–339). Since lipid mediator ceramide emerged as potent inducer apoptosis, we aimed at investigating occurrence formation and its potential...
Sarcopenia, the age-related loss of skeletal muscle mass, is associated with lipid accumulation and anabolic resistance; phenomena also observed in obesity worsen when aging are combined. The endocannabinoid system (ECS) overactivated obesity, but its role obesity-related dysfunction unknown. aims this study were to evaluate effect inhibition ECS by rimonabant (RIM) on metabolic alterations induced a high-fat high-sucrose diet mass/function aged mice. Eighteen-month-old male mice subjected...
Recently, it was demonstrated that CD8+ T cells are important for the response against Chlamydia pneumoniae. By use of human monocytic cell line U937 and monocytes taken from peripheral blood, we investigated effect infection on various molecules critical function. A strong secretion interleukin (IL)—10 by infected observed, together with an inhibited expression major histocompatibility complex (MHC) class I antigens, but without significant alteration tumor growth factor—β or MHC II...
The properties of coenzymes Q (CoQ9 and CoQ10) are closely linked to their redox state (CoQox/total CoQ) × 100. In this work, CoQ was biologically validated by high performance liquid chromatography‐electrochemical measurement after modulation mitochondrial electron flow cultured cells molecules increasing (rotenone, carbonyl cyanide chlorophenylhydrazone) or decreasing (antimycin) oxidation. tissue specificity content were investigated in control hypoxic rats. rats, there a strong negative...
Adipose tissues are differently involved in lipid metabolism and obesity according to their type location. Increasing reports stress on the impact of redox metabolic syndrome. The aim this work is investigate site‐specific three different adipose its changes occurring obesity. We analysed enzymatic non‐enzymatic parameters, focused reduced/oxidized glutathione coenzyme Q couples. In lean compared with obese non‐diabetic Zucker rats, interscapular brown fat seems well protected against...
Coenzyme Q (CoQ) is not only the single antioxidant synthesized in humans but also an obligatory element of mitochondrial functions. We have previously reported CoQ deficiency white adipose tissue ob/ob mice. sought to determine (i) whether this deficit exists all species and its relevance human obesity (ii) what extent could be involved adipocyte differentiation. Here we identified rodents as well a specific very strong nonlinear negative correlation between content subcutaneous indexes....
Lipids are essential cellular constituents that have many critical roles in physiological functions. They notably involved energy storage and cell signaling as second messengers, they major of membranes, including lipid rafts. As a consequence, implicated large number heterogeneous diseases, such cancer, diabetes, neurological disorders, inherited metabolic diseases. Due to the high structural diversity complexity species, presence isomeric isobaric their occurrence at concentration scale,...