A5067625437- Muscle metabolism and nutrition
- Diet and metabolism studies
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Cannabis and Cannabinoid Research
- Metabolism and Genetic Disorders
- PI3K/AKT/mTOR signaling in cancer
- Nutrition and Health in Aging
- Clinical Nutrition and Gastroenterology
- Parkinson's Disease Mechanisms and Treatments
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Biochemical effects in animals
- Genetics and Neurodevelopmental Disorders
- Nutrition, Genetics, and Disease
- Genetic Associations and Epidemiology
- Diabetes and associated disorders
- Restless Legs Syndrome Research
- Vitamin D Research Studies
- Proteins in Food Systems
- Epigenetics and DNA Methylation
- Protein Hydrolysis and Bioactive Peptides
- Endoplasmic Reticulum Stress and Disease
- Coenzyme Q10 studies and effects
Centre de Recherche en Nutrition Humaine d'Auvergne
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2007-2025
Unité de Nutrition Humaine
2016-2025
Université Clermont Auvergne
2016-2025
Centre Hospitalier Universitaire de Clermont-Ferrand
2021
Génétique, Diversité, Écophysiologie des Céréales
2016
Inserm
2001-2014
Université de Lille
2011-2014
European Genomic Institute for Diabetes
2012-2014
Université Lille Nord de France
2012
The most common pathology associated with obesity is insulin resistance, which results in the onset of type 2 diabetes mellitus. Several studies have implicated mammalian target rapamycin (mTOR) signaling pathway obesity. Eukaryotic translation initiation factor 4E–binding (eIF4E-binding) proteins (4E-BPs), repress by binding to eIF4E, are downstream effectors mTOR. We report that combined disruption 4E-BP1 and 4E-BP2 mice increased their sensitivity diet-induced Increased adiposity was...
Mounting evidence links deregulated protein synthesis to tumorigenesis via the translation initiation factor complex eIF4F. Components of this are often overexpressed in a large number cancers and promote malignant transformation experimental systems. mTOR affects activity eIF4F by phosphorylating repressors complex, eIF4E binding proteins. The immunosuppressant rapamycin specifically inhibits retards cancer growth. Importantly, mutations upstream negative regulators cause hamartomas,...
Skeletal muscle mitochondrial function is the biggest component of whole-body energy output. Mitochondrial production during exercise impaired in vitamin D-deficient subjects. In cultured myotubes, loss D receptor (VDR) decreases respiration rate and ATP from oxidative phosphorylation. We aimed to examine effects deficiency supplementation on expenditure old rats, mice, human To gain further insight into mechanisms involved, we used C2C12 cells transgenic mice with muscle-specific VDR...
It was recently demonstrated that TLR4 activation via dietary lipids triggers inflammatory pathway and alters insulin responsiveness in the fat tissue during obesity. Here, we question whether other TLR family members could participate TLR-mediated processes occurring obese adipose tissue. We thus studied expression of TLR1, TLR2, TLR4, TLR6 These receptors are expressed omental subcutaneous human tissue, being higher independently metabolic status subject. a correlation TLRs within between...
To determine whether circulating citrulline can be manipulated in vivo humans, and, if so, availability affects the levels of related amino acids, nitric oxide, urinary citrulline, and urea nitrogen, 10 healthy volunteers were studied on 3 separate days: 1) under baseline conditions; 2) after a 24-h treatment with phenylbutyrate (0.36 g.kg(-1).day(-1)), glutamine "trapping" agent; 3) during oral L-citrulline supplementation (0.18 randomized order. Plasma, erythrocyte (RBC), concentrations...
Type 2 diabetes manifests when the β-cell fails to secrete sufficient amounts of insulin maintain normoglycemia and undergoes apoptosis. The disease progression results from an interplay environmental factors genetic predisposition. Polymorphisms in T-cell factor 7-like (TCF7L2) strongly correlate with type mellitus (T2DM). While TCF7L2 mRNA is upregulated islets diabetes, protein levels are downregulated. loss induces impaired function By analyzing human isolated islets, we provide three...
This study used polarized cell line Caco-2 as a model of human enterocytes to determine: 1) whether deprivation nutrients on the apical (luminal) side epithelium (fasting) alters protein synthesis in enterocytes; 2) if so, glutamine can attenuate effects fasting; and 3) depend its route (i.e., vs. basolateral) supply. cells were submitted nutrient mimic fasting, whereas basolateral remained exposed regular medium. Cells then incubated with [ 2 H 3 ]leucine or without glutamine, fractional...
Cancer and chemotherapy induce a severe loss of muscle mass (known as cachexia), which negatively impact cancer treatment patient survival. The aim the present study was to investigate whether cannabidiol (CBD) administration may potentially antagonize effects cisplatin in inducing atrophy, using model myotubes culture. Cisplatin resulted reduction myotube diameter (15.7 ± 0.3 vs. 22.2 0.5 µm,
Regulated associated protein of mTOR (Raptor) and rapamycin-insensitive companion (rictor) are two proteins that delineate different complexes, mTORC1 mTORC2 respectively. Recent studies demonstrated the role rictor in development function β-cells. has long been known to impact β-cell development. However, most evaluating its used either drug treatment (i.e. rapamycin) or modification expression modulate activity, direct raptor insulin secretion is unclear. In this study, using siRNA, we...
Zinc ions are essential for the formation of insulin crystals in pancreatic β cells, thereby contributing to packaging efficiency stored insulin. fluxes regulated through SLC30A (zinc transporter, ZNT) family. Here, we investigated effect metabolic stress associated with prediabetic state depletion, glucotoxicity, and lipotoxicity) on ZNT expression human islet function. Both zinc depletion lipotoxicity (but not glucotoxicity) downregulated ZNT8 ( SLC30A8 ) altered glucose-stimulated...
Sarcopenia, the age-related loss of skeletal muscle mass, is associated with lipid accumulation and anabolic resistance; phenomena also observed in obesity worsen when aging are combined. The endocannabinoid system (ECS) overactivated obesity, but its role obesity-related dysfunction unknown. aims this study were to evaluate effect inhibition ECS by rimonabant (RIM) on metabolic alterations induced a high-fat high-sucrose diet mass/function aged mice. Eighteen-month-old male mice subjected...
The phenotype of sarcopenic obesity is frequently associated with impaired muscle strength and performance. Ectopic lipid deposition may interfere anabolic response especially during aging. Evidence scarce concerning the potential interplay among aging nutrient imbalance on skeletal functionality. objective present study was to investigate impact protein intake in context an obesogenic diet functional properties intramuscular infiltration. Two groups forty-two adult thirty-seven old male...
To assess the effect of glutamine availability on rates protein synthesis in human enterocytes, Caco-2 cells were grown until differentiation and then submitted to deprivation produced by exposure glutamine-free medium or methionine sulfoximine [l- S-[3-amino-3-carboxypropyl]- S-methylsulfoximine (MSO)], a synthetase inhibitor. Cells incubated with 2 H 3 -labeled leucine without glutamine, fractional rate (FSR) total cell was determined from enrichments protein-bound intracellular free...
Abstract Background Sarcopenia is the loss of muscle mass/function that occurs during aging process. The links between mechanistic target rapamycin (mTOR) activity and development are largely documented, but role its downstream targets in sarcopenia poorly understood. Eukaryotic initiation factor 4E‐binding proteins (4E‐BPs) mTOR repress mRNA translation involved control several physiological processes. However, their skeletal still goal this study was to assess how 4E‐BP1 4E‐BP2 expression...
Plant proteins are attracting rising interest due to their pro-health benefits and environmental sustainability. However, little is known about the nutritional value of pea when consumed by older people. Herein, we evaluated digestibility efficiency compared casein whey in old rats. Thirty 20-month-old male Wistar rats were assigned an isoproteic isocaloric diet containing either (CAS), soluble milk protein (WHEY) or Pisane™ isolate for 16 weeks. The three had a similar effect on nitrogen...