A5026716563- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Pancreatic function and diabetes
- Peroxisome Proliferator-Activated Receptors
- Diabetes and associated disorders
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- interferon and immune responses
- Adipose Tissue and Metabolism
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Nuclear Receptors and Signaling
- DNA Repair Mechanisms
- Enterobacteriaceae and Cronobacter Research
- Antioxidant Activity and Oxidative Stress
- Microbial Community Ecology and Physiology
- Neuroscience and Neuropharmacology Research
- Diabetes Management and Research
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- NF-κB Signaling Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory mediators and NSAID effects
- Diabetes Treatment and Management
Université de Lille
2009-2024
Inserm
2009-2024
Centre Hospitalier Universitaire de Lille
2012-2024
Institut Pasteur de Lille
2004-2024
Centre National de la Recherche Scientifique
2022-2024
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2002-2024
National Institutes of Health
1999-2024
Lille Neurosciences & Cognition
2022
Centre de Recherche Jean Pierre Aubert
2014-2021
5-aminosalicylic acid (5-ASA) is an antiinflammatory drug widely used in the treatment of inflammatory bowel diseases. It known to inhibit production cytokines and mediators, but mechanism underlying intestinal effects 5-ASA remains unknown. Based on common activities peroxisome proliferator–activated receptor-γ (PPAR-γ) ligands 5-ASA, we hypothesized that this nuclear receptor mediates therapeutic action. To test possibility, colitis was induced heterozygous PPAR-γ+/− mice their wild-type...
Nucleic acid protection is a substantial challenge for neurons, which are continuously exposed to oxidative stress in the brain. Neurons require powerful mechanisms protect DNA and RNA integrity ensure their functionality longevity. Beside its well known role microtubule dynamics, we recently discovered that Tau also key nuclear player of neuronal genomic under reactive oxygen species (ROS)-inducing heat (HS) conditions primary cultures. In this report, analyzed capacity vivo adult mice...
Abstract Pericentromeric heterochromatin (PCH) gives rise to highly dense chromatin sub-structures rich in the epigenetic mark corresponding trimethylated form of lysine 9 histone H3 (H3K9me3) and protein 1α (HP1α), which regulate genome expression stability. We demonstrate that Tau, a involved number neurodegenerative diseases including Alzheimer’s disease (AD), binds localizes within or next neuronal PCH primary cultures from wild-type mice. Concomitantly, we show clustered distribution...
Twenty strains of Enterobacteriaceae isolated from water are assigned to a new species in the genus Serratia on basis phenetic (numerical analysis) and genetic (deoxyribonucleic acid [DNA]-DNA hybridization) evidence. They closely related each other (75 91% DNA-DNA relatedness) show 50% DNA relatedness with but only 20 30% members family Enterobacteriaceae. The acetoin, gelatinase, deoxyribonuclease negative, lysine, ornithine decarboxylase, Tween-esterase positive. metabolically very...
The objective of this trial was to study the effects fenofibrate (FF) and gemfibrozil (GF), most commonly used fibrates, on high-density lipoprotein (HDL) apolipoprotein (apo) A-I.In a head-to-head double-blind clinical trial, both FF GF decreased triglycerides increased HDL cholesterol levels similar extent, whereas plasma apoA-I only after but not GF. Results in human (h) apoA-Itransgenic (hA-ITg) peroxisome proliferator-activated receptor (PPAR) alpha-/- mice demonstrated that PPARalpha...
Transcription factors of the interferon regulatory factor (IRF) family bind to type I (IFN)responsive element (ISRE) and activate transcription from IFN-inducible genes.To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed nuclear extracts prepared tissue culture cells.The results demonstrated endogenous IRFs bound ISRE are complexed histone acetylases, PCAF, GCN5, p300/CREB protein acetylase activities accumulated on IRF-ISRE complexes.By testing...
Tauopathies such as Alzheimer's Disease (AD) are neurodegenerative disorders for which there is presently no cure. They named after the abnormal oligomerization/aggregation of neuronal microtubule-associated Tau protein. Besides its role a protein, DNA-binding capacity and nuclear localization protein has been described in neurons. While questioning potential Tau-DNA binding development tauopathies, we have carried out large-scale analysis interaction with genome under physiological heat...
Insoluble intracellular aggregation of tau proteins into filaments and neurodegeneration are histopathological hallmarks Alzheimer disease (AD) other tauopathies. Recently, prefibrillar, soluble, oligomeric intermediates have emerged as relevant pathological species; however, the molecular mechanisms neuronal responses to oligomers not fully understood. Here, we show that hippocampal neurons in six-month-old transgenic mouse model tauopathy, THY-Tau22, enriched with tau, contain elongated...
Obese patients have chronic, low-grade inflammation that predisposes to type 2 diabetes and results, in part, from dysregulated visceral white adipose tissue (WAT) functions. The specific signaling pathways underlying WAT dysregulation, however, remain unclear. Here we report the PPARgamma pathway operates differently of lean obese mice. visceral, but not subcutaneous, mice displayed increased sensitivity activation by its agonist rosiglitazone. This correlated with expression gene encoding...
While the transmembrane glycoprotein mucin 1 (MUC1) is clustered at apical borders of normal epithelial cells, with transformation and loss polarity, MUC1 found high levels in cytosol uniformly distributed over entire surface carcinoma where it can promote tumor progression adversely affects response to therapy. Clear cell renal (ccRCC), main histotype kidney cancer, typically highly resistant conventional targeted therapies for reasons that remain largely unknown. In this context, we...
In this study, we used an immunodeficient mouse model to explore, in vivo, the longitudinal adaptation of human islets obesogenic environment. Non-diabetic Rag2 –/– mice (n = 61) were transplanted with (400 islet equivalents [IEQ]) from six pancreases: four non-diabetic and two overt metabolic dysfunction (older, high HbAlc or history diabetes). Animals fed for 12 weeks a control high-fat diet (HFD), followed weight, serum triacylglycerol, fasting blood glucose C-peptide. After killed,...
Multiple lines of evidence have linked oxidative stress, tau pathology and neuronal cell cycle re-activation to Alzheimer's disease (AD). While a prevailing idea is that stress-induced reactivation acts as an upstream trigger for pathological phosphorylation, others identified inducer abnormalities in both mitotic postmitotic conditions. In addition, nuclear hypophosphorylated has been key player the DNA damage response stress. Whether what extent these observations are causally remains...
Rosiglitazone displays powerful antidiabetes benefits but is associated with increased body weight and adipogenesis. Keeping in mind the concept of selective peroxisome proliferator-activated receptor (PPAR)gamma modulator, aim this study was to characterize properties a new PPARgamma ligand, S 26948, special attention body-weight gain.We used transient transfection binding assays characterized characteristics 26948 GST pull-down experiments investigate its pattern coactivator recruitment...
It has been shown that adult pancreatic ductal cells can dedifferentiate and act as progenitors. Dedifferentiation of epithelial is often associated with the epithelial-mesenchymal transition (EMT). In this study, we investigated occurrence EMT in human exocrine both vitro vivo. Cells fraction isolated from pancreas brain-dead donors were first cultured suspension for eight days. This led to formation spheroids, composed a principal population duct-like phenotype. When cultivated tissue...
Regulated associated protein of mTOR (Raptor) and rapamycin-insensitive companion (rictor) are two proteins that delineate different complexes, mTORC1 mTORC2 respectively. Recent studies demonstrated the role rictor in development function β-cells. has long been known to impact β-cell development. However, most evaluating its used either drug treatment (i.e. rapamycin) or modification expression modulate activity, direct raptor insulin secretion is unclear. In this study, using siRNA, we...
Zinc ions are essential for the formation of insulin crystals in pancreatic β cells, thereby contributing to packaging efficiency stored insulin. fluxes regulated through SLC30A (zinc transporter, ZNT) family. Here, we investigated effect metabolic stress associated with prediabetic state depletion, glucotoxicity, and lipotoxicity) on ZNT expression human islet function. Both zinc depletion lipotoxicity (but not glucotoxicity) downregulated ZNT8 ( SLC30A8 ) altered glucose-stimulated...