A5006129290- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pharmacogenetics and Drug Metabolism
- RNA modifications and cancer
- Acute Lymphoblastic Leukemia research
- Circular RNAs in diseases
- Childhood Cancer Survivors' Quality of Life
- Renal Transplantation Outcomes and Treatments
- Medical Imaging and Pathology Studies
- Acute Myeloid Leukemia Research
- Renal Diseases and Glomerulopathies
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Drug Transport and Resistance Mechanisms
- Organ Transplantation Techniques and Outcomes
- Pharmacological Effects and Toxicity Studies
- Chronic Kidney Disease and Diabetes
- Neonatal Respiratory Health Research
- Caveolin-1 and cellular processes
- Cancer therapeutics and mechanisms
- Colorectal Cancer Treatments and Studies
- Cell Adhesion Molecules Research
Centre Hospitalier Universitaire de Lille
2016-2025
Université de Lille
2016-2025
Institut Pasteur de Lille
2012-2025
Centre National de la Recherche Scientifique
2020-2025
Inserm
2020-2025
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2021-2025
Allen Institute for Brain Science
2024
Institut du Cerveau
2023-2024
Université Côte d'Azur
2009-2024
Assistance Publique – Hôpitaux de Paris
2023
As miRNAs are associated with normal cellular processes, deregulation of is thought to play a causative role in many complex diseases. Nevertheless, the precise contribution fibrotic lung diseases, especially idiopathic form (IPF), remains poorly understood. Given poor response rate IPF patients current therapy, new insights into pathogenic mechanisms controlling fibroblasts activation, key cell type driving fibrogenic process, essential develop therapeutic strategies for this devastating...
Epithelial-mesenchymal interactions are critical in regulating many aspects of vertebrate embryo development, and for the maintenance homeostatic equilibrium adult tissues. The between epithelium mesenchyme believed to be mediated by paracrine signals such as cytokines extracellular matrix components secreted from fibroblasts that affect adjacent epithelia. In this study, we sought identify repertoire microRNAs (miRNAs) normal lung human their potential regulation TNF-alpha, IL-1beta...
MicroRNAs (miRNAs) are a class of noncoding RNA acting at post-transcriptional level to control the expression large sets target mRNAs. While there is evidence that miRNAs deregulation plays causative role in various complex disorders, their fibrotic kidney diseases largely unexplored. Here, we found strong up-regulation miR-21 kidneys mice with unilateral ureteral obstruction and also patients severe fibrosis. In addition, mouse primary fibroblasts derived from exhibited higher compared...
Rationale: Given the paucity of effective treatments for idiopathic pulmonary fibrosis (IPF), new insights into deleterious mechanisms controlling lung fibroblast activation, key cell type driving fibrogenic process, are essential to develop therapeutic strategies. TGF-β (transforming growth factor-β) is main profibrotic factor, but its inhibition associated with severe side effects because pleiotropic role. Objectives: To determine if downstream noncoding effectors in fibroblasts may...
Variability in genes involved drug pharmacokinetics or response can be responsible for suboptimal treatment efficacy predispose to adverse reactions. In addition common genetic variations, large-scale sequencing studies have uncovered multiple rare variants predicted cause functional alterations encoding proteins implicated metabolism, transport and response. To understand the importance of DPYD, a pharmacogene whose severe toxicity patients exposed fluoropyrimidine-based regimens, massively...
Renal proximal tubular epithelial cells play a central role in renal physiology and are among the cell types most sensitive to ischemia xenobiotic nephrotoxicity. In order investigate molecular cellular mechanisms underlying pathophysiology of kidney injuries, stable well-characterized primary culture model is required. An existing hampered by heterogeneity kidney; method based on sorting for specific markers must therefore be developed. this study, we present mechanical enzymatic...
Numerous lung cell lines are currently used as in vitro models for pharmacological and toxicological studies. However, no exhaustive report about the metabolic capacities of these comparison with those tissues is available. In present study, we a high-throughput quantitative real-time reverse transcription-polymerase chain reaction strategy to characterize expression profiles 380 genes encoding proteins involved metabolism disposition xenobiotics 10 commonly (A549, H292, H358, H460, H727,...
Abstract IgA nephropathy (IgAN) is the most prevalent primary glomerulonephritis, whose prognosis highly variable. Interstitial fibrosis a strong independent factor. Among microRNA involved in renal fibrogenesis, only few have been investigated IgAN. In context of IgAN, we aimed to analyze role miR-21-5p, miR-214-3p and miR-199a-5p, three established “fibromiRs” fibrosis. Fifty-six IgAN biopsy specimens were retrospectively scored according Oxford classification. Renal expression miR-199a-5p...
Background Childhood acute lymphoblastic leukemia (ALL) is the most common cancer in children, and can now be cured approximately 80% of patients. Nevertheless, drug resistance major cause treatment failure children with ALL. The methotrexate (MTX), which widely used to treat many human cancers, essentially all protocols worldwide for newly diagnosed Although MTX has been extensively studied years, relatively little known about mechanisms de novo primary cells, including cells. This lack...
MicroRNAs (miRNAs) have recently emerged as novel regulators in liver fibrosis. miR-30c and miR-193 are involved fibrotic remodeling processes cancer development, respectively. This study aimed to explore the role of fibrosis.The regulation miRNAs carbon tetrachloride-induced fibrosis was analyzed by microarray. Expression patterns were further confirmed samples obtained from two murine models hepatic human tissues. On a functional level, miRNA levels context transforming growth factor...
Telomeres are nucleoprotein structures at the end of chromosomes. The telomerase complex, constituted catalytic subunit TERT, RNA matrix hTR and several cofactors, including H/ACA box ribonucleoproteins Dyskerin, NOP10, GAR1, NAF1 NHP2, regulates telomere length. In humans, inherited defects in length maintenance responsible for a wide spectrum clinical premature aging manifestations pulmonary fibrosis (PF), dyskeratosis congenita (DC), bone marrow failure predisposition to cancer. NHP2...
Background Interstitial lung diseases (ILDs) include a large number of associated with progressive pulmonary fibrosis (PPF), including idiopathic (IPF). Despite the rarity each fibrotic ILDs individually, they cumulatively affect considerable patients. PPF is characterised by an excessive collagen deposition leading to functional decline. Objectives Therapeutic options are limited nintedanib and pirfenidone which only able reduce progression. CD206-expressing M2 macrophages involved in...
BackgroundGlucocorticoids are used in the curative treatment of acute lymphoblastic leukemia (ALL). Resistance to glucocorticoids is an important adverse prognostic factor newly diagnosed ALL patients but its mechanism unknown. Because SWI/SNF complex–mediated chromatin remodeling required for glucocorticoid transcriptional activity vitro, we investigated whether expression subunits complex was related resistance ALL.
Renal cell carcinoma, the most common neoplasm of adult kidney, accounts for about 3% malignancies and is usually highly resistant to conventional therapy. MicroRNAs are a class small non-coding RNAs, which have been previously shown promote malignant initiation progression. In this study, we focused our attention on miR-21, well described oncomiR commonly upregulated in cancer. Using cohort 99 primary renal carcinoma samples, showed that miR-21 expression cancer tissues was higher than...
While the transmembrane glycoprotein mucin 1 (MUC1) is clustered at apical borders of normal epithelial cells, with transformation and loss polarity, MUC1 found high levels in cytosol uniformly distributed over entire surface carcinoma where it can promote tumor progression adversely affects response to therapy. Clear cell renal (ccRCC), main histotype kidney cancer, typically highly resistant conventional targeted therapies for reasons that remain largely unknown. In this context, we...