A5013471585- Pharmacogenetics and Drug Metabolism
- Cancer-related molecular mechanisms research
- Renal Transplantation Outcomes and Treatments
- MicroRNA in disease regulation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Circular RNAs in diseases
- Inflammatory mediators and NSAID effects
- Organ Transplantation Techniques and Outcomes
- Eicosanoids and Hypertension Pharmacology
- Medical Imaging and Pathology Studies
- Renal and related cancers
- Renal Diseases and Glomerulopathies
- RNA modifications and cancer
- Chemotherapy-induced organ toxicity mitigation
- Biochemical and Molecular Research
- Neonatal Respiratory Health Research
- Glutathione Transferases and Polymorphisms
- Birth, Development, and Health
- Chronic Kidney Disease and Diabetes
- Childhood Cancer Survivors' Quality of Life
- Hormonal Regulation and Hypertension
- Acute Lymphoblastic Leukemia research
- HIV/AIDS drug development and treatment
Université de Lille
2015-2024
Centre Hospitalier Universitaire de Lille
2000-2024
Institut Pasteur de Lille
2023-2024
Centre National de la Recherche Scientifique
2020-2024
CANTHER - Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers
2021-2024
Inserm
2020-2024
Onco Lille
2021
IMPact de l'Environnement Chimique sur la Santé humaine
2016-2019
Institut de Biologie de Lille
2013-2015
Département de Chimie Moléculaire
2013
As miRNAs are associated with normal cellular processes, deregulation of is thought to play a causative role in many complex diseases. Nevertheless, the precise contribution fibrotic lung diseases, especially idiopathic form (IPF), remains poorly understood. Given poor response rate IPF patients current therapy, new insights into pathogenic mechanisms controlling fibroblasts activation, key cell type driving fibrogenic process, essential develop therapeutic strategies for this devastating...
MicroRNAs (miRNAs) are a class of noncoding RNA acting at post-transcriptional level to control the expression large sets target mRNAs. While there is evidence that miRNAs deregulation plays causative role in various complex disorders, their fibrotic kidney diseases largely unexplored. Here, we found strong up-regulation miR-21 kidneys mice with unilateral ureteral obstruction and also patients severe fibrosis. In addition, mouse primary fibroblasts derived from exhibited higher compared...
Rationale: Given the paucity of effective treatments for idiopathic pulmonary fibrosis (IPF), new insights into deleterious mechanisms controlling lung fibroblast activation, key cell type driving fibrogenic process, are essential to develop therapeutic strategies. TGF-β (transforming growth factor-β) is main profibrotic factor, but its inhibition associated with severe side effects because pleiotropic role. Objectives: To determine if downstream noncoding effectors in fibroblasts may...
Variability in genes involved drug pharmacokinetics or response can be responsible for suboptimal treatment efficacy predispose to adverse reactions. In addition common genetic variations, large-scale sequencing studies have uncovered multiple rare variants predicted cause functional alterations encoding proteins implicated metabolism, transport and response. To understand the importance of DPYD, a pharmacogene whose severe toxicity patients exposed fluoropyrimidine-based regimens, massively...
Renal proximal tubular epithelial cells play a central role in renal physiology and are among the cell types most sensitive to ischemia xenobiotic nephrotoxicity. In order investigate molecular cellular mechanisms underlying pathophysiology of kidney injuries, stable well-characterized primary culture model is required. An existing hampered by heterogeneity kidney; method based on sorting for specific markers must therefore be developed. this study, we present mechanical enzymatic...
Abstract IgA nephropathy (IgAN) is the most prevalent primary glomerulonephritis, whose prognosis highly variable. Interstitial fibrosis a strong independent factor. Among microRNA involved in renal fibrogenesis, only few have been investigated IgAN. In context of IgAN, we aimed to analyze role miR-21-5p, miR-214-3p and miR-199a-5p, three established “fibromiRs” fibrosis. Fifty-six IgAN biopsy specimens were retrospectively scored according Oxford classification. Renal expression miR-199a-5p...
The effect of potentially relevant genetic polymorphisms, CYP3A5 6986A>G and ABCB1 3435C>T, on Tacrolimus pharmacokinetics graft clinical outcome was investigated in donor recipient DNA samples from 209 kidney transplant patients.The mean follow-up 21.8 ± 9 months. dose, trough blood concentrations (C0) C0/dose ratio were only statistically correlated with the genotype. genotypes appeared to have no influence incidence Biopsy Proven Acute Rejection Delayed Graft Function. Renal function not...
Renal cell carcinoma, the most common neoplasm of adult kidney, accounts for about 3% malignancies and is usually highly resistant to conventional therapy. MicroRNAs are a class small non-coding RNAs, which have been previously shown promote malignant initiation progression. In this study, we focused our attention on miR-21, well described oncomiR commonly upregulated in cancer. Using cohort 99 primary renal carcinoma samples, showed that miR-21 expression cancer tissues was higher than...
Abstract Pro‐aging effects of endogenous advanced glycation end‐products (AGEs) have been reported, and there is increasing interest in the pro‐inflammatory ‐fibrotic their binding to RAGE (the main AGE receptor). The role dietary AGEs aging remains ill‐defined, but predominantly renal accumulation carboxymethyllysine (CML) suggests kidneys may be particularly affected. We studied impact invalidation a CML‐enriched diet on aging. Two‐month‐old male, wild‐type (WT) −/− C57Bl/6 mice were fed...
While the transmembrane glycoprotein mucin 1 (MUC1) is clustered at apical borders of normal epithelial cells, with transformation and loss polarity, MUC1 found high levels in cytosol uniformly distributed over entire surface carcinoma where it can promote tumor progression adversely affects response to therapy. Clear cell renal (ccRCC), main histotype kidney cancer, typically highly resistant conventional targeted therapies for reasons that remain largely unknown. In this context, we...
Aims To identify the molecular basis for a low CYP1A2 metabolic status, as determined by caffeine phenotyping test, in 71‐year‐old, nonsmoking, Caucasian woman who presented with very high clozapine concentrations despite being administered standard dose of drug. Methods The nucleotide sequence 7 exons, exon‐intron boundaries and 5′‐flanking region gene was analysed direct sequencing. Results Only one heterozygous point mutation identified donor splice site intron 6 (3534 G > A) . This...
Five novel natural allelic variants -951A>C, 1042G>A (D348N), 1156A>T (I386F), 1217G>A (C406Y) and 1291C>T
Cadmium is an environmental pollutant well known for its nephrotoxic effects. Nevertheless, mechanisms underlying nephrotoxicity continue to be elucidated. MicroRNAs (miRNAs) have emerged in recent years as modulators of xenobiotic-induced toxicity. In this context, our study aimed at elucidating whether miRNAs are involved renal proximal tubular toxicity induced by cadmium exposure. We showed that exposure, 2 distinct cell models (renal epithelial [RPTEC]/human telomerase reverse...
Non-small cell lung cancer is characterized by a dismal prognosis largely owing to inefficient diagnosis and tenacious drug resistance. Therefore, the identification of new molecular determinants underlying sensitivity cells existing therapy particular importance develop effective combinatorial treatment strategy. MicroRNAs (miRNAs), class small non-coding RNAs, have been established as master regulators variety cellular processes that play key role in tumor initiation, progression...