A5080109601- Air Quality and Health Impacts
- Pharmacogenetics and Drug Metabolism
- Energy and Environment Impacts
- Air Quality Monitoring and Forecasting
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Smoking Behavior and Cessation
- Carcinogens and Genotoxicity Assessment
- Drug Transport and Resistance Mechanisms
- Biochemical and Molecular Research
- Glutathione Transferases and Polymorphisms
- Climate Change and Health Impacts
- Eicosanoids and Hypertension Pharmacology
- Toxic Organic Pollutants Impact
- Acute Lymphoblastic Leukemia research
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Pharmaceutical studies and practices
- Noise Effects and Management
- Proteoglycans and glycosaminoglycans research
- Cystic Fibrosis Research Advances
- Childhood Cancer Survivors' Quality of Life
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Chemical Reactions and Isotopes
- Metabolomics and Mass Spectrometry Studies
Institut Pasteur de Lille
2016-2025
Université de Lille
2016-2025
IMPact de l'Environnement Chimique sur la Santé humaine
2016-2024
Centre Hospitalier Universitaire de Lille
2002-2024
Institut Pasteur
2024
Université Lille Nord de France
2009-2019
Institut de Biologie de Lille
2019
Laboratoire de Génétique Cellulaire
2019
Hôpital Albert Calmette
1995-2012
Université du littoral côte d'opale
2012
The polymorphic cytochrome P450 CYP2D6 is involved in the metabolism of various drugs wide therapeutic use and a presumed susceptibility factor for certain environmentally-induced diseases. Our aim was to define mutations alleles gene evaluate their frequencies European population. Using polymerase chain reaction-single strand conformation polymorphism analysis, 672 unrelated subjects were screened 9 exons exon-intron boundaries. A total 48 point identified, which 29 novel. Mutations 1749...
As miRNAs are associated with normal cellular processes, deregulation of is thought to play a causative role in many complex diseases. Nevertheless, the precise contribution fibrotic lung diseases, especially idiopathic form (IPF), remains poorly understood. Given poor response rate IPF patients current therapy, new insights into pathogenic mechanisms controlling fibroblasts activation, key cell type driving fibrogenic process, essential develop therapeutic strategies for this devastating...
Epithelial-mesenchymal interactions are critical in regulating many aspects of vertebrate embryo development, and for the maintenance homeostatic equilibrium adult tissues. The between epithelium mesenchyme believed to be mediated by paracrine signals such as cytokines extracellular matrix components secreted from fibroblasts that affect adjacent epithelia. In this study, we sought identify repertoire microRNAs (miRNAs) normal lung human their potential regulation TNF-alpha, IL-1beta...
MicroRNAs (miRNAs) are a class of noncoding RNA acting at post-transcriptional level to control the expression large sets target mRNAs. While there is evidence that miRNAs deregulation plays causative role in various complex disorders, their fibrotic kidney diseases largely unexplored. Here, we found strong up-regulation miR-21 kidneys mice with unilateral ureteral obstruction and also patients severe fibrosis. In addition, mouse primary fibroblasts derived from exhibited higher compared...
Electronic cigarette use has raised concern worldwide regarding potential health risks and its position in tobacco cessation strategies. As part of any toxicity assessment, the chemical characterization e-liquids their related vapors are among fundamental data to be determined. Considering lack available reference methods, we developed validated several analytical procedures order conduct a multicomponent analysis six e-liquid refills resultant vapor emissions (generated by smoking machine),...
Characterization of allelic variants the TPMT gene ( ) responsible for changes in activity, and elucidation mechanism by which these alleles act, are required because clinical importance this polymorphism patients receiving thiopurine drugs. We defined mutational spectrum a group 191 Europeans. Using PCR–SSCP, we screened mutation entire coding sequence, exon‐intron boundaries, promoter region 3′‐flanking gene. Six mutations were detected throughout ten exons seven characterized. Four them,...
Mucin glycopeptides were prepared from the sputum of a patient suffering cystic fibrosis.The carbohydrate chains released by alkaline borohydride treatment.Sialylated and sulfated oligosaccharide-alditols purified ion-exchange chromatography, gel filtration, high performance anion-exchange chromatography.The structures determined resolution 'H N M R spectroscopy in combinatlon with fast atom bombardmentmass spectrometry.Twenty-four oligosaccharides characterized illustrate diversity fibrosis...
Numerous lung cell lines are currently used as in vitro models for pharmacological and toxicological studies. However, no exhaustive report about the metabolic capacities of these comparison with those tissues is available. In present study, we a high-throughput quantitative real-time reverse transcription-polymerase chain reaction strategy to characterize expression profiles 380 genes encoding proteins involved metabolism disposition xenobiotics 10 commonly (A549, H292, H358, H460, H727,...
Nowadays ambient particulate matter (PM) levels still regularly exceed the guideline values established by World Health Organization in most urban areas. Numerous experimental studies have already demonstrated airway toxicity of fine fraction PM (FP), mainly triggered oxidative stress-induced inflammation. However, only few actually paid close attention to ultrafine (UFP), which is likely be more easily internalized cells and biologically reactive. Mitochondria are major endogenous sources...
To detect mutations in the thiopurine S-methyltransferase gene (TPMT), we have developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of amplified by polymerase chain reaction (PCR). The sensitivity method was first evaluated analyzing DNA samples from five individuals, including two high methylators (HMs), intermediate (IMs), and one deficient methylator (DM). TPMT alleles each these individuals had previously been characterized conventional PCR-based assays...
Intestinal cell lines are used as in vitro models for pharmacological and toxicological studies. However, a general report of the gene expression spectrum proteins that involved metabolism disposition xenobiotics these systems is not currently available. To fill this information gap, we systematically characterized profile 377 genes encoding xenobiotic-metabolizing enzymes, transporters, nuclear receptors transcription factors intestinal mucosa (ileum, ascending colon, transverse descending...
Metabolite identification in untargeted metabolomics is complex, with the risk of false positive annotations. This work aims to use machine learning successively predict retention time (Rt) and collision cross-section (CCS) an open-access database accelerate interpretation metabolomic results. Standards metabolites were tested using liquid chromatography coupled high-resolution mass spectrometry. In CCSBase QSRR predictor models, experimental results used generate predicted CCS Rt Human...
ABSTRACT HT-29 cells selected by adaptation to 10−5 M methotrexate (HT-29 MTX) are a homogeneous cell population producing high amounts of mucin. Intracellular mucins and proteoglycans were isolated from these ultra-centrifugation lysates on cesium bromide gradient further separated anion-exchange perfomance liquid chromatography. The major mucin fraction was characterized hydroxy amino acid content (40%), Thr/Ser ratio 1.52, sialic content, low sulfate content. When the same procedure...
We compared the chemical composition of salivary mucin glycopeptides from cystic fibrosis (CF) and non-CF subjects adhesion Pseudomonas aeruginosa to these different glycopeptides. Three pools CF saliva, four one individual saliva were studied. The soluble fraction was treated with pronase, gel filtration performed obtain high low molecular mass yield total significantly higher than saliva. Furthermore, revealed a sialic acid content in glycopeptides, sulfate fucose glycopeptides.We studied...