A5010639330- Pharmacogenetics and Drug Metabolism
- Glutathione Transferases and Polymorphisms
- Chemical Reactions and Isotopes
- Epigenetics and DNA Methylation
- Veterinary medicine and infectious diseases
- Social Media and Politics
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Treatments and Mutations
- Acute Lymphoblastic Leukemia research
- Biochemical and Molecular Research
- Gastric Cancer Management and Outcomes
- Computational Drug Discovery Methods
- Hate Speech and Cyberbullying Detection
- Blood groups and transfusion
- Hemoglobinopathies and Related Disorders
- Drug Transport and Resistance Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Statistical Methods in Clinical Trials
- E-Government and Public Services
- Helicobacter pylori-related gastroenterology studies
- Systemic Lupus Erythematosus Research
- Liver Disease Diagnosis and Treatment
- Analytical Chemistry and Chromatography
World Health Organization Regional Office for the Eastern Mediterranean
2011
Laboratoire de Biochimie
1998
Centre Hospitalier Universitaire de Lille
1995-1998
Calmette Hospital
1998
Laboratoire de Biochimie
1998
Biologie Labor
1997
Hôpital Albert Calmette
1995
The polymorphic cytochrome P450 CYP2D6 is involved in the metabolism of various drugs wide therapeutic use and a presumed susceptibility factor for certain environmentally-induced diseases. Our aim was to define mutations alleles gene evaluate their frequencies European population. Using polymerase chain reaction-single strand conformation polymorphism analysis, 672 unrelated subjects were screened 9 exons exon-intron boundaries. A total 48 point identified, which 29 novel. Mutations 1749...
To detect mutations in the cytochrome P450 CYP2D6 gene (CYP2D6), we developed a strategy based on single-strand conformation polymorphism (SSCP) analysis of amplified by polymerase chain reaction (PCR). The efficiency method was evaluated analysing DNA samples from extensive metabolizers (EM) and poor (PM) debrisoquine. Haplotypes, alleles had previously been characterized each individual using PCR assays, Xba I restriction fragment length (RFLP) sequencing. PCR-SSCP results were complete...
Purpose: The objective of this study was to explore netizens' external and internal political efficacy as being exposed branding on social media show how would level up the citizens’ awareness boost their participation in voting parliamentary election. Lebanon taken a case study. Theoretical framework: Recent literature has reported impact youth while other studies have been done separately about particularly developed countries. (Binder et al., 2021; Hassan & Ridzuan, Moorman 2019;...
A novel loss-of function allele of the CYP2D6 gene was characterized in a PM individual using exon-by-exon PCR-SSCP analysis. This allele, we termed CYP2D6(F), harbours four mutations including new mutation (D6-F) which abolishes splice acceptor site 1st intron and results premature stop codon. DNA samples from large population healthy unrelated volunteers were tested for D6-F PCR-assay developed specific identification genomic DNA. The prevalence very low. However, its combined with that...
Many studies have been performed in an attempt to establish a link between the polymorphism of cytochrome P45O CYP2D6 gene and incidence lung cancer. Nevertheless, whether or not this genetic has role development disease remains unclear. Recently, new advances our knowledge its locus (CYP2D) achieved. In particular, was found be highly polymorphic multiple novel mutations allelic variants identified. addition, number CYP2D rearrangements, including those with amplification gene,...
The possible association between lung cancer and the CYP2D6*9 mutant allele, which reduces catalytic activity of cytochrome P450 CYP2D6, was examined by PCR-SSCP using peripheral blood DNA from 249 cases 265 controls, with detailed data on smoking. allele present in 4.9% controls 6% cases. Adjusted for age, hospital smoking, odds ratio (OR) associated presence 12 [95% confidence interval (CI) 0.5–2.9]. According to histological type, adenocarcinoma small cell carcinoma were not a...
Earlier reports on the detection of Helicobacter DNA in gallbladder tissue patients with biliary diseases have shown discordant results. This study aimed to detect presence gallstone, and bile specimens from subjects H. pylori-positive gastritis cholelithiasis. The pylori antrum biopsies was confirmed by rapid urease test and/or histopathological examination. extracted gallbladder, gallstone samples 50 undergoing cholecystectomy. genus-specific (16S rRNA genes) determined nested polymerase...
Previous reports of an association between the polymorphic cytochrome P450 CYP2D6 and systemic lupus erythematosus are conflicting. Following elucidation molecular basis genetic polymorphism, we re-examined hypothesis this gene with a susceptibility to by analysing complete coding sequence. For purpose, studied occurrence 16 mutations in genomic DNA from 69 patients large control group using previously described polymerase chain reaction-single strand confirmation polymorphism analysis. In...