A5090109192- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Bone health and treatments
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- Alzheimer's disease research and treatments
- Hedgehog Signaling Pathway Studies
- Fibroblast Growth Factor Research
- Axon Guidance and Neuronal Signaling
- Renal and related cancers
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Muscle Physiology and Disorders
- Bone fractures and treatments
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Advanced Proteomics Techniques and Applications
- Genomic variations and chromosomal abnormalities
- Immunotherapy and Immune Responses
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
KU Leuven
2019-2025
IMEC
2024
The Francis Crick Institute
2021
Fibrosis and fat replacement in skeletal muscle are major complications that lead to a loss of mobility chronic disorders, such as muscular dystrophy. However, the vivo properties adipogenic stem precursor cells remain unclear, mainly due high cell heterogeneity muscles. Here, we use single-cell RNA sequencing decomplexify interstitial populations healthy dystrophic We identify an CD142-positive population mice humans is responsible for inhibition adipogenesis through GDF10 secretion....
Abstract Regulatory CD4 + T cells (Treg) prevent tumor clearance by conventional (Tconv) comprising a major obstacle of cancer immune-surveillance. Hitherto, the mechanisms Treg repertoire formation in human cancers remain largely unclear. Here, we analyze clonal origin breast patients using T-Cell Receptor and single-cell transcriptome sequencing. While peripheral blood tumors are clonally distinct, Tconv clones, including tumor-antigen reactive effectors (Teff), detected both compartments....
ABSTRACT The frequent acquisition of genomic abnormalities in human preimplantation embryos is a leading cause pregnancy loss, but does not necessarily prohibit healthy offspring. However, the impact on cellular states and development early embryo remains largely unclear. Here, we characterise aneuploidy reconstruct gene regulatory networks embryos, investigate expression developmental perturbations instigated by using single-cell genome-and-transcriptome sequencing (G&T-seq). At level,...
Multiple lines of evidence have linked oxidative stress, tau pathology and neuronal cell cycle re-activation to Alzheimer's disease (AD). While a prevailing idea is that stress-induced reactivation acts as an upstream trigger for pathological phosphorylation, others identified inducer abnormalities in both mitotic postmitotic conditions. In addition, nuclear hypophosphorylated has been key player the DNA damage response stress. Whether what extent these observations are causally remains...
Abstract Single-cell multi-omics methods enable the study of cell state diversity, which is largely determined by interplay genome, epigenome, and transcriptome. Here, we describe Gtag&T-seq, a genome-and-transcriptome sequencing (G&T-seq) protocol same single cells that omits whole-genome amplification (WGA) using direct genomic tagmentation (Gtag). Gtag drastically decreases cost improves coverage uniformity at single-cell pseudo-bulk levels compared to WGA-based G&T-seq. We...
Abstract Recent advances in spatial omics methods are revolutionising biomedical research by enabling detailed molecular analyses of cells and their interactions native state. As most technologies capture only a specific type molecules, there is an unmet need to enable integration multiple spatial-omics datasets. This, however, presents several challenges as these typically operate on separate tissue sections at disparate resolutions. Here, we established multi-omics pipeline co-registration...
ABSTRACT Single-cell multi-omics methods are enabling the study of cell state diversity, which is largely determined by interplay genome, epigenome, and transcriptome. Here, we describe Gtag&T-seq, a genome-and-transcriptome sequencing (G&T-seq) protocol same single cells that omits whole-genome amplification (WGA) using direct genomic tagmentation (Gtag). Gtag drastically decreases cost improves coverage uniformity at both single-cell pseudo-bulk level when compared to WGA-based...