Xander Spotbeen
A5040319024- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Ferroptosis and cancer prognosis
- Analytical Chemistry and Chromatography
- Prostate Cancer Treatment and Research
- Molecular Biology Techniques and Applications
- Immunotherapy and Immune Responses
- Immune cells in cancer
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Melanoma and MAPK Pathways
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
- MicroRNA in disease regulation
- Computational Drug Discovery Methods
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
KU Leuven
2020-2024
South Australian Health and Medical Research Institute
2020-2023
VIB-KU Leuven Center for Cancer Biology
2023
Abstract Dysregulated lipid metabolism is a prominent feature of prostate cancer that driven by androgen receptor (AR) signaling. Here we used quantitative mass spectrometry to define the “lipidome” in tumors with matched benign tissues (n = 21), independent unmatched 47), and primary explants cultured clinical AR antagonist enzalutamide 43). Significant differences composition were detected spatially visualized compared samples. Notably, featured higher proportions monounsaturated lipids...
Abstract Prostate cancer treatment resistance is a significant challenge facing the field. Genomic and transcriptomic profiling have partially elucidated mechanisms through which cells escape treatment, but their relation toward tumor microenvironment (TME) remains elusive. Here we present comprehensive landscape of prostate TME at multiple points in standard timeline employing single-cell RNA-sequencing spatial transcriptomics data from 120 patients. We identify club-like as key epithelial...
A major therapeutic barrier in melanoma is the coexistence of diverse cellular states marked by distinct metabolic traits. Transitioning from a proliferative to an invasive phenotype coupled with increased ferroptosis vulnerability. However, regulatory circuits controlling susceptibility across cell are unknown. In this work, we identified Apolipoprotein E ( APOE ) as top lipid-metabolism gene segregating MITF high /AXL low proliferative/ferroptosis-resistant invasive/ferroptosis-sensitive...
One of the key limitations targeted cancer therapies is rapid onset therapy resistance. Taking BRAF-mutant melanoma as paradigm, we previously identified lipogenic regulator SREBP-1 a central mediator resistance to MAPK-targeted therapy. Reasoning that lipogenesis-mediated alterations in membrane lipid poly-unsaturation lie at basis resistance, fatty acid synthase (FASN) player this pathway evoke an exquisite vulnerability clinical inducers reactive oxygen species (ROS), thereby...
Abstract Recent advances in spatial omics methods are revolutionising biomedical research by enabling detailed molecular analyses of cells and their interactions native state. As most technologies capture only a specific type molecules, there is an unmet need to enable integration multiple spatial-omics datasets. This, however, presents several challenges as these typically operate on separate tissue sections at disparate resolutions. Here, we established multi-omics pipeline co-registration...
Abstract Matrix‐assisted laser/desorption ionisation‐mass spectrometry imaging (MALDI‐MSI) enables label‐free of biomolecules in biological tissues. However, many molecules remain undetected due to their poor ionisation efficiencies. These efficiencies practically limit spatial resolution. Herein, we address this challenge for aromatic antioxidants by reporting an innovative approach involving sequential matrix‐assisted laser desorption and two‐photon desorbed neutrals. It is shown that ion...
Abstract Dysregulated lipid metabolism is a prominent feature of prostate cancer that driven by androgen receptor (AR) signaling. Herein, we used quantitative mass spectrometry to define the “lipidome” in tumors with matched benign tissues (n=21), independent (n=47), and primary explants cultured clinical AR antagonist, enzalutamide (n=43). Significant differences composition were detected spatially visualized compared samples. Notably, featured higher proportions monounsaturated lipids...
Abstract Prostate cancer treatment resistance is a significant challenge facing the field. Genomic and transcriptomic profiling have partially elucidated mechanisms through which cells escape treatment, but their relation toward tumor microenvironment (TME) remains elusive. Here we present comprehensive landscape of prostate TME at multiple points in standard timeline employing single-cell RNA-sequencing spatial transcriptomics data from 110 patients. We identify club-like as key epithelial...
<div>Abstract<p>Dysregulated lipid metabolism is a prominent feature of prostate cancer that driven by androgen receptor (AR) signaling. Here we used quantitative mass spectrometry to define the “lipidome” in tumors with matched benign tissues (<i>n</i> = 21), independent unmatched 47), and primary explants cultured clinical AR antagonist enzalutamide 43). Significant differences composition were detected spatially visualized compared samples. Notably, featured higher...
<p>Supplementary Figures S1-S6</p>
<p>Supplementary Figures S1-S6</p>
<div>Abstract<p>Dysregulated lipid metabolism is a prominent feature of prostate cancer that driven by androgen receptor (AR) signaling. Here we used quantitative mass spectrometry to define the “lipidome” in tumors with matched benign tissues (<i>n</i> = 21), independent unmatched 47), and primary explants cultured clinical AR antagonist enzalutamide 43). Significant differences composition were detected spatially visualized compared samples. Notably, featured higher...
Abstract Membranes are unique phospholipid (PL) interfaces that play a central role in cancer cell biology. However, PL composition of clinical tumors, and its dynamic regulation, remains critical gap the molecular profiling this disease. Here, we used mass spectrometry-based spatial imaging lipidomics to generate profiles prostate tissues upon development patient-derived tumor explants (PDEs; n=43) cultured with current agent enzalutamide. Analysis more than 100 species tumors matched...
Abstract Tumor growth is inevitably accompanied by changes in the tumor-microenvironment to which cancer cells have adapt order thrive. Alterations metabolism and blood perfusion of solid tumors been suggested drive a spontaneous increase tumoral temperature. However, it currently unknown if this phenomenon affects biology. We found increased temperature human pancreatic ductal adenocarcinoma (PDAC) tumors. By mimicking observation PDAC cell lines, we that altering cellular lipidome...