A5019952642- Digestive system and related health
- Cancer Cells and Metastasis
- Viral gastroenteritis research and epidemiology
- 3D Printing in Biomedical Research
- Clinical Nutrition and Gastroenterology
- Epigenetics and DNA Methylation
- Escherichia coli research studies
- Hedgehog Signaling Pathway Studies
- Inflammatory Bowel Disease
- Microplastics and Plastic Pollution
- Infant Nutrition and Health
- Diet and metabolism studies
- Pancreatic and Hepatic Oncology Research
- Air Quality and Health Impacts
- Immune cells in cancer
- Pancreatic function and diabetes
- Congenital gastrointestinal and neural anomalies
- Graphene and Nanomaterials Applications
- Cystic Fibrosis Research Advances
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Helicobacter pylori-related gastroenterology studies
- SARS-CoV-2 and COVID-19 Research
- Liver Disease Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
University of New Mexico
2018-2024
Johns Hopkins University
2013-2022
Johns Hopkins Medicine
2013-2022
Nephrology Center of Maryland
2021
Johns Hopkins Hospital
2020
University of Pittsburgh
2017
University of Baltimore
2016
Catholic University of America
2006-2014
University of America
2006-2014
Shriners Hospitals for Children - Boston
2006
Human gastrointestinal tract research is limited by the paucity of in vitro intestinal cell models that recapitulate cellular diversity and complex functions human physiology disease pathology. enteroid (HIE) cultures contain multiple epithelial types comprise epithelium (enterocytes goblet, enteroendocrine, Paneth cells) are physiologically active based on responses to agonists. We evaluated these nontransformed, three-dimensional HIE as for pathogenic infections small intestine examining...
Abstract Organ interactions resulting from drug, metabolite or xenobiotic transport between organs are key components of human metabolism that impact therapeutic action and toxic side effects. Preclinical animal testing often fails to predict adverse outcomes arising sequential, multi-organ drugs xenobiotics. Human microphysiological systems (MPS) can model these predicted dramatically improve the efficiency drug development process. In this study, five MPS models were evaluated for...
Enterohemorrhagic E. coli (EHEC) causes over 70,000 episodes of foodborne diarrhea annually in the USA. The early sequence events which precede life-threatening hemorrhagic colitis and hemolytic uremic syndrome are not fully understood due to initial asymptomatic phase disease lack a suitable animal model. aim this study was determine molecular interaction between EHEC human colonic epithelium.Human colonoids derived from adult proximal stem cells were developed into monolayers...
Global plastic use has consistently increased over the past century with several different types of plastics now being produced. Much these end up in oceans or landfills leading to a substantial accumulation environment. Plastic debris slowly degrades into microplastics (MPs) that can ultimately be inhaled ingested by both animals and humans. A growing body evidence indicates MPs cross gut barrier enter lymphatic systemic circulation tissues such as lungs, liver, kidney, brain. The impacts...
Background & AimsOne of the features ulcerative colitis (UC) is a defect in protective mucus layer. This has been attributed to reduced number goblet cells (GCs). However, it not known whether abnormal GC secretion also contributes Our aims were investigate was UC and exists as long-term effect chronic inflammation.MethodsColonoids established from intestinal stem healthy subjects (HS) patients with UC. Colonoids maintained undifferentiated (UD) or induced differentiate (DF) studied...
Abstract Microplastics represent an emerging environmental contaminant, with large gaps in our understanding of human health impacts. Furthermore, factors may modify the plastic chemistry, further altering toxic potency. Ultraviolet (UV) light is one such unavoidable factor for airborne microplastic particulates and a known modifier polystyrene surface chemistry. As experimental model, we aged commercially available microspheres 5 weeks UV radiation, then compared cellular responses A549...
Abstract EAEC is a common cause of diarrheal illness worldwide. Pathogenesis believed to occur in the ileum and colon, where bacteria adhere form robust aggregating biofilm. Among multiple virulence factors produced by EAEC, Pic serine protease has been implicated bacterial colonization virtue its mucinolytic activity. Hence, potential role mucus barrier disruption during infection long postulated. In this study, we used human colonoids comprising goblet cells thick mucin as an intestinal...
Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of intestinal epithelium. Due to their closed structures and significant supporting extracellular matrix, 3D not ideal for host-pathogen studies. Enteroids colonoids can be grown as epithelial monolayers on permeable tissue culture membranes allow manipulation both luminal basolateral cell surfaces accompanying fluids. This enhanced surface accessibility...
Breastfeeding has been associated with long lasting health benefits. Nutrients and bioactive components of human breast milk promote cell growth, immune development, shield the infant gut from insults microbial threats. The molecular cellular events involved in these processes are ill defined. We have established pediatric enteroids interrogated maternal milk’s impact on epithelial maturation function comparison commercial formula. Colostrum applied apically to enteroid monolayers reduced...
Intestinal enteroids are ex vivo primary cultured single-layer epithelial cell spheroids of average diameter ∼150 μm with luminal surface facing inward. Measurement enteroid swelling in response to secretagogues has been applied genetic testing cystic fibrosis and evaluation drug candidates for secretory diarrheas. The current measurement method involves manual addition drugs solutions embedded a Matrigel matrix estimation volume changes from confocal images fluorescently stained enteroids....
Liver injury is accompanied by replication and apoptosis, molecular processes which remove replace the damaged cells.MRI CT can detect structural abnormalities in liver but cannot differentiate abscesses from metabolically active lesions.Likewise, it difficult to between metabolism using PET FDG or FLT.Recently, radiolabeled FIAU (2-fluro-2-deoxy-1 beta-D-arabinofuranosyl-5iodouracil) has been examined a number of groups for detection tumors various animal models.Likewise, annexin used...
Human 3-dimensional (3D) enteroid or colonoid cultures derived from crypt base stem cells are currently the most advanced ex vivo model of intestinal epithelium. Due to their closed structures and significant supporting extracellular matrix, 3D not ideal for host-pathogen studies. Enteroids colonoids can be grown as epithelial monolayers on permeable tissue culture membranes allow manipulation both luminal basolateral cell surfaces accompanying fluids. This enhanced surface accessibility...
Global plastic use has consistently increased over the past century with several different types of plastics now being produced. Much these end up in oceans or landfills leading to a substantial accumulation environment. Plastic debris slowly degrades into microplastics (MPs) that can ultimately be inhaled ingested by both animals and humans. A growing body evidence indicates MPs cross gut barrier enter lymphatic systemic circulation tissues such as lungs, liver, kidney, brain. The impacts...
Life-threatening intestinal and systemic effects of the Shiga toxins produced by enterohemorrhagic Escherichia coli (EHEC) require toxin uptake transcytosis across epithelial cells. We have recently demonstrated that EHEC infection cells stimulates macropinocytosis, an actin-dependent endocytic pathway. Host actin rearrangement necessary for attachment to enterocytes is mediated type 3 secretion system which functions as a molecular syringe translocate bacterial effector proteins directly...