A5035026466- Cancer Genomics and Diagnostics
- Evolution and Genetic Dynamics
- Geomechanics and Mining Engineering
- Genomics and Phylogenetic Studies
- Geoscience and Mining Technology
- CRISPR and Genetic Engineering
- Rock Mechanics and Modeling
- Epigenetics and DNA Methylation
- Mathematical Biology Tumor Growth
- Genetic factors in colorectal cancer
- Chromosomal and Genetic Variations
- Hippo pathway signaling and YAP/TAZ
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Civil and Geotechnical Engineering Research
- Science, Research, and Medicine
- Grouting, Rheology, and Soil Mechanics
- Gene expression and cancer classification
- Geotechnical and Geomechanical Engineering
- Offshore Engineering and Technologies
- Environmental DNA in Biodiversity Studies
- earthquake and tectonic studies
- Single-cell and spatial transcriptomics
- Earthquake Detection and Analysis
University of Science and Technology Beijing
2022
University of Washington
2017-2021
Chinese Academy of Sciences
2008-2020
Beijing Institute of Genomics
2008-2020
University of Chinese Academy of Sciences
2019
Next-generation sequencing methods suffer from low recovery, uneven coverage, and false mutations. DNA fragmentation by sonication is a major contributor to these problems because it produces randomly sized fragments, PCR amplification bias, end artifacts. In addition, oligonucleotide-based hybridization capture, common target enrichment method, has limited efficiency for small genomic regions, contributing recovery. This becomes critical problem in clinical applications, which value...
Cells of multi-cellular organisms evolve toward uni-cellularity in the form cancer and, if humans intervene, continue to cell culture. During this process, gene dosage relationships may novel ways cope with new environment and regress back ancestral uni-cellular state. In context, evolution sex chromosomes vis-a-vis autosomes is particular interest. Here, we report chromosomal ~ 600 lines. Many them jettisoned either Y or inactive X; thus, free-living male female cells converge by becoming...
Chromosomal inversion is an important type of genomic variations involved in both evolution and disease pathogenesis. Here, we describe the refined genetic structure a 3.8-Mb polymorphism at chromosome 8p23. Using HapMap data 1,073 SNPs generated from 209 unrelated samples CEPH-Utah residents with ancestry northern western Europe (CEU); Yoruba Ibadan, Nigeria (YRI); Asian (ASN) samples, which were comprised Han Chinese Beijing, China (CHB) Japanese Tokyo, Japan (JPT)-we successfully deduced...
Large genomes with elevated mutation rates are prone to accumulating deleterious mutations more rapidly than natural selection can purge (Muller's ratchet). As a consequence, it may lead the extinction of small populations. Relative most unicellular organisms, cancer cells, large and nonrecombining genome high rate, could be particularly susceptible such "mutational meltdown." However, common type in organismal evolution, namely, mutation, has received relatively little attention biology...
Abstract Classical r- vs. K-selection theory describes the trade-offs between high reproductive output and competitiveness guides research in evolutionary ecology. While its impact has waned recent past, cancer evolution may rekindle it. Herein, we impose or on cell lines to obtain strongly proliferative r cells highly competitive K test ideas life-history strategy evolution. RNA-seq indicates that are associated with distinct expression of genes involved cycle, adhesion, apoptosis, contact...
ABSTRACT Current next-generation sequencing techniques suffer from inefficient target enrichment and frequent errors. To address these issues, we have developed a targeted genome fragmentation approach based on CRISPR/Cas9 digestion. By designing all fragments to similar lengths, regions of interest can be size-selected prior library preparation, increasing hybridization capture efficiency. Additionally, homogenous length reduce PCR bias maximize read usability. We combine this novel with...
Abstract Cells of multi-cellular organisms evolve toward uni-cellularity in the form cancer and, if humans intervene, continue to cell culture. During this process, gene dosage relationships may novel ways cope with new environment and regress back ancestral unicellular state. In context, evolution sex chromosomes vis-a-vis autosomes is particular interest. Here, we report chromosomal ~600 lines. Many them jettisoned either Y or inactive X; thus, free-living male female cells converge by...
Abstract Classical r - vs. K -selection theory describes the trade-offs between high reproductive output and competitiveness guides research in evolutionary ecology 1–5 . While its impact has waned recent past, cancer evolution may rekindle it 6–10 Indeed, solid tumors are an ideal theater for and, hence, a good testing ground ideas on life-history strategy 11,12 In this study, we impose or HeLa cells to obtain strongly proliferative highly competitive cells. RNA-seq analysis indicates that...
Abstract Populations accumulate deleterious mutations that have yet to be removed by natural selection. This mutated portion of the population does not contribute its fitness is called genetic load and proportional mutation rate. Given genomic instability cancer cells, could substantial if indeed leads loss cell fitness. We hypothesize because aneuploidy copy number variations (CNVs) are most common forms genome instability, their consequences may determine in cells. To test this hypothesis,...
ABSRACT Large and non-recombining genomes are prone to accumulating deleterious mutations faster than natural selection can purge (Muller’s ratchet). A possible consequence would then be the extinction of small populations. Relative most single-cell organisms, cancer cells, with large genomes, could particularly susceptible such “mutational meltdown”. Curiously, in cells rarely noticed despite strong signals genome sequences. Here, by monitoring clones from HeLa cell lines, we characterize...
Abstract Objective: The development of Pap tests as a molecular diagnostic for serous ovarian cancer is promising concept, but previous studies reported limited sensitivity. In addition, the presence cancer-associated somatic mutations in normal tissue increasingly recognized specificity challenge mutational tests. We aimed to determine performance detection using ultra-accurate deep sequencing. Methods: used CRISPR-DS, novel duplex sequencing method that employs CRISPR-based target...
Abstract A new approach to future FLNG is presented which will significantly reduce the overall CAPEX, EPCIC schedule, and OPEX compared past conventional bespoke designs project execution methods. Using a standardized design on both topside Hull, this concept provide of liquefaction process modules with flexible hull forms cargo containment systems. This can be deployed around world near shore or offshore Jetty Single Point Mooring, such as SSY an External Turret. The standardization...