A5020466456- Asthma and respiratory diseases
- Advanced Proteomics Techniques and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer, Hypoxia, and Metabolism
- Pulmonary Hypertension Research and Treatments
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Allergic Rhinitis and Sensitization
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Inflammation biomarkers and pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Respiratory and Cough-Related Research
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Occupational and environmental lung diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- Acute Lymphoblastic Leukemia research
- Cancer Immunotherapy and Biomarkers
- Context-Aware Activity Recognition Systems
Mayo Clinic in Arizona
2021-2025
Xiangya Hospital Central South University
2024
Central South University
2024
Delta Air Lines (United States)
2020-2024
Mayo Clinic
2023-2024
WinnMed
2024
XinHua Hospital
2006-2024
Shanghai Jiao Tong University
2022
University of Minnesota Rochester
2021
University Hospital of Basel
2009-2017
Abstract We have developed NetPath as a resource of curated human signaling pathways. As an initial step, provides detailed maps number immune pathways, which include approximately 1,600 reactions annotated from the literature and more than 2,800 instances transcriptionally regulated genes - all linked to over 5,500 published articles. anticipate become consolidated for pathways that should enable systems biology approaches.
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis. The kinase inhibitor nintedanib specific for vascular endothelial growth factor receptor (VEGFR), platelet-derived (PDGFR) and fibroblast (FGFR) significantly reduced the rate of decline forced vital capacity versus placebo.To determine in vitro effect on primary human fibroblasts.Fibroblasts were isolated from lungs IPF patients non-fibrotic controls. We assessed VEGF, PDGF-BB basic FGF (bFGF) ± on: (i)...
Hypoxia-inducible factor 1 (HIF-1) activates transcription of genes encoding proteins that play key roles in breast cancer biology. We hypothesized interaction HIF-1 with epigenetic regulators may increase transcriptional activity, and thereby promote progression. report the histone demethylase jumonji domain containing protein 2C (JMJD2C) selectively interacts HIF-1α, but not HIF-2α, HIF-1α mediates recruitment JMJD2C to hypoxia response elements target genes. decreases trimethylation H3 at...
Hypoxia-inducible factors (HIFs) are transcription that mediate adaptive responses to reduced oxygen availability. HIF-alpha subunits stabilized under conditions of acute hypoxia. However, prolonged hypoxia leads decay HIF-1alpha but not HIF-2alpha protein levels by unknown mechanisms. Here, we identify Hsp70 and CHIP (carboxyl terminus Hsc70-interacting protein) as HIF-1alpha-interacting proteins. Hsp70, through recruiting the ubiquitin ligase CHIP, promotes ubiquitination proteasomal...
Identification of phosphorylated proteins remains a difficult task despite technological advances in protein purification methods and mass spectrometry. Here, we report identification tyrosine-phosphorylated by coupling stable isotope labeling with amino acids cell culture (SILAC) to We labeled HeLa cells isotopes tyrosine, or, combination arginine lysine identify tyrosine proteins. This allowed 118 proteins, which only 45 were previously described as A total 42 vivo phosphorylation sites...
Adipose tissue plays a key role as fat-storage depot and an endocrine organ. Although mouse adipogenesis has been studied extensively, limited studies have conducted to characterize this process in humans. We carried out temporal proteomic analysis interrogate the dynamic changes secretome of primary human preadipocytes they differentiate into mature adipocytes. Using iTRAQ-based quantitative proteomics, we identified quantified 420 proteins from differentiated Our results revealed that...
Thymic stromal lymphopoietin (TSLP) is a four-helix bundle cytokine that plays critical role in the regulation of immune responses and differentiation hematopoietic cells. TSLP signals through heterodimeric receptor complex consisting an interleukin-7 α chain unique (TSLPR) [also known as receptor-like factor 2 (CRLF2)]. Cellular targets include dendritic cells, B mast regulatory T (Treg) cells CD4+ CD8+ The TSLP/TSLPR axis can activate multiple signaling transduction pathways including...
Background In allergic asthma, IgE increases airway remodelling but the mechanism is incompletely understood. Airway consists of two independent events increased cell numbers and enhanced extracellular matrix deposition, by which up-regulates proliferation deposition human smooth muscle cells in asthma unclear. Objective Characterise role receptors associated signalling cascades remodelling. Methods Primary (8 asthmatics, 8 non-asthmatics) were stimulated with purified antibody-activated...
In Alzheimer disease brain the microtubule associated protein (MAP) tau is abnormally hyperphosphorylated. The role of phosphatases (PP) in regulation phosphorylation was studied undifferentiated SY5Y cells. cells treated with 10 nM okadaic acid (OA), a PP-2A/PP-1 inhibitor, PP-1 and -2A activities decreased by 60% 100% respectively MAPKs, cdc2 kinase cdk5, but not GSK-3, increased. OA increased at Thr-231/Ser-235 Ser-3961404, Ser-262/356 or Ser-199/202. An increase tyrosinated/detyrosinated...
Silver nanoparticles (11+/-1.5 nm) could greatly enhance the electron-transfer reactivity of myoglobin (Mb) and its catalytic ability toward hydrogen peroxide (H2O2). Direct fast electron transfer between Mb a pyrolytic graphite (PG) electrode was achieved, pair well-defined, quasi-reversible redox peaks obtained. The cathodic anodic were located at -329 -281 mV, respectively. Meanwhile, protein reduction H2O2 also studied, biosensor subsequently fabricated. Its detection limit 1.0 x 10(-6)...
Abstract Pleural malignant mesothelioma is a locally aggressive tumor of mesothelial cell origin. In other types high expression matrix metalloproteinase (MMP)‐2, together with membrane‐type1‐MMP (MT1‐MMP), and low levels the tissue inhibitor MMP (TIMP)‐2 have been correlated progression survival rates. Therefore, we compared activation these three factors their regulation by two associated growth factors, platelet‐derived factor (PDGF)‐BB, transforming (TGF)‐β 1 in six human one line....
The modification of intracellular proteins by monosaccharides O ‐linked β‐ N ‐acetylglucosamine ( O‐ GlcNAc) is an essential and dynamic PTM metazoans. addition removal ‐GlcNAc catalyzed the transferase (OGT) ‐GlcNAcase, respectively. One mechanism which thought to mediate regulating phosphorylation. To provide insight into pathways regulated ‐GlcNAc, we have utilized SILAC‐based quantitative proteomics carry out comparisons site‐specific phosphorylation in OGT wild‐type Null cells....
Airway wall remodeling in allergic asthma is reduced after treatment with humanized anti-IgE-antibodies. We reported earlier that purified IgE, without the presence of allergens, sufficient to induce airway due smooth muscle cell (ASMC) activity deposing extracellular matrix.We postulate IgE contained serum patients, absence stimulates ASMC activities and can be prevented by anti-IgE antibodies.Isolated human were exposed obtained from: (i) healthy controls, or patients (ii) asthma, (iii)...
// Xinyan Wu 1, 2, * , Muhammad Saddiq Zahari Binyun Ma 6 Ren Liu Santosh Renuse 5 Nandini A. Sahasrabuddhe Lily Chen 3 Raghothama Chaerkady 2 Min-Sik Kim Jun Zhong Christine Jelinek Mustafa Barbhuiya Pamela Leal-Rojas 7 Yi Yang Manoj Kumar Kashyap Arivusudar Marimuthu Min Ling 1 Mary Jo Fackler Vanessa Merino Zhen Zhang Cynthia Zahnow Edward Gabrielson 3, 4 Vered Stearns Juan Carlos Roa 8 Saraswati Sukumar Parkash S. Gill Akhilesh Pandey Department of Biological Chemistry, Johns Hopkins...
Liver fibrosis is characterized by the activation of perivascular hepatic stellate cells (HSCs), release fibrogenic nanosized extracellular vesicles (EVs), and increased HSC glycolysis. Nevertheless, how glycolysis in HSCs coordinates amplification through tissue zone-specific pathways remains elusive. Here, we demonstrate that HSC-specific genetic inhibition reduced liver fibrosis. Moreover, spatial transcriptomics revealed a fibrosis-mediated up-regulation EV-related pericentral zone,...
During infection and cancer, mTORC1-mediated metabolic regulation impacts CD8+ T cell effector expansion memory development. However, the mechanisms by which cells regulate mTORC1 to support their unique requirements remain unknown. Here we show that NKG7, a lysosomal protein whose expression is restricted cytotoxic lymphocytes, negatively regulates recruitment activation inhibiting assembly function of proton pump, vacuolar ATPase (v-ATPase). Human mouse lacking NKG7 more acidic lysosomes...