A5036963997- Proteoglycans and glycosaminoglycans research
- Protease and Inhibitor Mechanisms
- Pulmonary Hypertension Research and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Retinal Diseases and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Retinal and Macular Surgery
- Peptidase Inhibition and Analysis
- Intraocular Surgery and Lenses
- Fibroblast Growth Factor Research
- Cancer Treatment and Pharmacology
- Glaucoma and retinal disorders
- Neuropeptides and Animal Physiology
- Microbial infections and disease research
- Connective tissue disorders research
- Neuroendocrine regulation and behavior
- Retinal Imaging and Analysis
- Hormonal and reproductive studies
- Blood Coagulation and Thrombosis Mechanisms
- Veterinary Equine Medical Research
- Sexual function and dysfunction studies
- Histiocytic Disorders and Treatments
Aristotle University of Thessaloniki
2011-2020
Indiana University School of Medicine
2012
University Hospital of Basel
2006
University of Patras
1988-2004
SKiN Health
2004
National and Kapodistrian University of Athens
1995
University of Vienna
1995
University of London
1982
Skin aging is a multifactorial process consisting of two distinct and independent mechanisms: intrinsic extrinsic aging. Youthful skin retains its turgor, resilience pliability, among others, due to high content water. Daily external injury, in addition the normal aging, causes loss moisture. The key molecule involved moisture hyaluronic acid (HA) that has unique capacity retaining There are multiple sites for control HA synthesis, deposition, cell protein association degradation, reflecting...
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis. The kinase inhibitor nintedanib specific for vascular endothelial growth factor receptor (VEGFR), platelet-derived (PDGFR) and fibroblast (FGFR) significantly reduced the rate of decline forced vital capacity versus placebo.To determine in vitro effect on primary human fibroblasts.Fibroblasts were isolated from lungs IPF patients non-fibrotic controls. We assessed VEGF, PDGF-BB basic FGF (bFGF) ± on: (i)...
The effect of the nitric oxide (NO)‐producing nitrovasodilators isosorbide mononitrate (ISMN) and dinitrate (ISDN) were assessed on (a) in vivo model angiogenesis chick chorioallantoic membrane (CAM) (b) growth metastatic properties Lewis Lung carcinoma (LLC) mice Isosorbide 5‐mononitrate (ISDN), inhibited CAM dose‐dependently. ISMN was more potent inhibiting this process. Both compounds capable completely reversing angiogenic α‐thrombin. These effects ISDN comparable to those previously...
Abstract: Extrinsic skin ageing or ‘photoageing’, as opposed to intrinsic ageing, is the result of exposure external factors, mainly ultraviolet irradiation. Glycosaminoglycans (GAG) and particularly hyaluronic acid (HA) are major components cutaneous extracellular matrix involved in tissue repair. However, their involvement extrinsic remains elusive. In this study, we investigated expression HA its metabolizing enzymes photoexposed photoprotected human specimens, obtained from same patient....
Total glycans from the cell layer and culture medium of human vascular smooth muscle cells (VSMC) that had been cultivated in presence platelet-derived growth factor (PDGF) were isolated purified by gel filtration after Pronase DNase digestion alkaliborohydride treatment. Measurements content neutral hexoses uronic acids revealed PDGF stimulates total glycan synthesis proliferating VSMC a linear fashion 24 h to 72 incubation. In contrast, fibroblasts, epithelial cells, or endothelial was not...
To investigate possible correlations between vitreous and/or serum levels of platelet-derived growth factor isoforms (PDGF-AA, -AB and -BB) with parameters associated non-proliferative diabetic retinopathy (NPDR) clinically significant macula oedema (CSMO); to compare the results relevant regarding vascular endothelial (VEGF), which is an established affecting NPDR.Fifteen patients NPDR, 31 proliferative (PDR) 15 non-diabetic were included in study. Vitreous samples obtained during...
Hypoxia is associated with extracellular matrix remodeling in several inflammatory lung diseases, such as fibrosis, chronic obstructive pulmonary disease, and asthma. In a human cell culture model, we assessed whether modification by hypoxia platelet-derived growth factor (PDGF) involves the action of metalloproteinases (MMPs) thereby affects proliferation. Expression MMP its activity were zymography enzyme-linked immunosorbent assay fibroblasts vascular smooth muscle cells (VSMCs),...
Purpose: We investigated possible correlations between vitreous and/or serum levels of platelet derived growth factor isoforms (PDGF-AA, -AB, -BB) with parameters associated proliferative diabetic retinopathy (PDR), and compared the results to vascular endothelial (VEGF), which is an established affecting PDR. Methods: Thirty-one patients PDR 15 non-diabetic were included in study. Vitreous samples obtained during vitrectomy. PDGF-AA, -BB, as well VEGF, measured by enzyme-linked...
Glycosaminoglycans (GAG) are essential extracellular matrix molecules which regulate tissue flexibility, a parameter that is reduced in airways of patients with asthma and chronic obstructive pulmonary disease (COPD). We investigated the expression GAG their metabolising enzymes primary human airway smooth muscle cells (ASMC) obtained from healthy donors (controls) or COPD. Total synthesis was assessed by [ 3 H]-glucosamine incorporation. were isolated, purified, fractionated electrophoresis...
Acute exacerbations of chronic obstructive pulmonary disease (AE-COPD) are associated with accelerated aggravation clinical symptoms and deterioration function. The mechanisms by which may contribute to airway remodeling declined lung function poorly understood. In this study, we investigated if AE-COPD differential expression matrix metalloproteinases (MMPs) their tissue inhibitors (TIMPs) in bronchoalveolar lavage (BAL). COPD patients undergoing diagnostic bronchoscopy, either stable (n =...
Idiopathic pulmonary arterial hypertension (IPAH) is a fatal disease characterised by elevated blood pressure in the circulation. Initial vasoconstriction, proliferation of smooth muscle cells (PASMC) and increased deposition extracellular matrix (ECM) contribute to pathological remodelling arterioles IPAH. Glycosaminoglycans (GAGs), components ECM, control cellular differentiation, but their expression IPAH remains elusive. In present study, GAG was investigated lungs patients with or...