A5079421874- Chronic Obstructive Pulmonary Disease (COPD) Research
- Proteoglycans and glycosaminoglycans research
- Asthma and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pulmonary Hypertension Research and Treatments
- Respiratory and Cough-Related Research
- Protease and Inhibitor Mechanisms
- Retinal Diseases and Treatments
- Glycosylation and Glycoproteins Research
- Respiratory Support and Mechanisms
- Neonatal Respiratory Health Research
- Retinal and Macular Surgery
- Fibroblast Growth Factor Research
- Inhalation and Respiratory Drug Delivery
- Pediatric health and respiratory diseases
- Peptidase Inhibition and Analysis
- Adipose Tissue and Metabolism
- Pleural and Pulmonary Diseases
- Glaucoma and retinal disorders
- Respiratory viral infections research
- Macrophage Migration Inhibitory Factor
- Dermatology and Skin Diseases
- Neuropeptides and Animal Physiology
- Intraocular Surgery and Lenses
- High Altitude and Hypoxia
University Medical Center Freiburg
2023-2025
University of Freiburg
2022-2025
University Hospital of Basel
2015-2024
University of Basel
2017-2024
Aristotle University of Thessaloniki
2014-2023
Thriasio General Hospital of Elefsina
2014-2023
Medizinische Hochschule Hannover
2016-2018
University of Dundee
2017
Ninewells Hospital
2017
University of Milan
2017
Skin aging is a multifactorial process consisting of two distinct and independent mechanisms: intrinsic extrinsic aging. Youthful skin retains its turgor, resilience pliability, among others, due to high content water. Daily external injury, in addition the normal aging, causes loss moisture. The key molecule involved moisture hyaluronic acid (HA) that has unique capacity retaining There are multiple sites for control HA synthesis, deposition, cell protein association degradation, reflecting...
Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis. The kinase inhibitor nintedanib specific for vascular endothelial growth factor receptor (VEGFR), platelet-derived (PDGFR) and fibroblast (FGFR) significantly reduced the rate of decline forced vital capacity versus placebo.To determine in vitro effect on primary human fibroblasts.Fibroblasts were isolated from lungs IPF patients non-fibrotic controls. We assessed VEGF, PDGF-BB basic FGF (bFGF) ± on: (i)...
Abstract: Extrinsic skin ageing or ‘photoageing’, as opposed to intrinsic ageing, is the result of exposure external factors, mainly ultraviolet irradiation. Glycosaminoglycans (GAG) and particularly hyaluronic acid (HA) are major components cutaneous extracellular matrix involved in tissue repair. However, their involvement extrinsic remains elusive. In this study, we investigated expression HA its metabolizing enzymes photoexposed photoprotected human specimens, obtained from same patient....
Abstract Background Growing numbers of post‐adolescent females are suffering from treatment‐resistant or relapsing adult acne forms, therefore requiring the definition safe and effective treatment options for this burdening disease. Objectives To assess efficacy azelaic acid 15% gel (AzA) vs. no during maintenance therapy female to compare its safety adapalene 0.1% ( AD ) a 9‐month period (3‐month 6‐month treatment). Methods A total 55 women between 18 45 years with were included in...
The patho-mechanism leading to airway wall remodeling in allergic asthma is not well understood and resistant therapies. This study assessed the effect of immunoglobulin E (IgE) absence allergens on human primary smooth muscle cell (ASMC) vitro. ASMCs were obtained from five patients controls. Proliferation was determined by direct counts, mitochondrial activity expression cytochrome c, protein immunoblotting immuno-fluorescence, migration microscopy imaging, collagen deposition based ELISA...
Abstract Background Bronchial thermoplasty regulates structural abnormalities involved in airway narrowing asthma. In the present study we aimed to investigate effect of bronchial on histopathological structures distinct asthma endotypes/phenotypes. Methods Endobronchial biopsies (n = 450) were collected from 30 patients with severe uncontrolled before and after 3 sequential thermoplasties. Patients classified based blood eosinophils, atopy, allergy smoke exposure. Tissue sections assessed...
Background Although inhaled corticosteroids (ICS) are highly effective in asthma, they provide significant, but modest, clinical benefit COPD. Here, we tested the hypothesis that high bronchial airway smooth muscle cell (ASMC) area COPD is associated with ICS responsiveness. Methods In this investigator-initiated and -driven, double-blind, randomised, placebo-controlled trial (HISTORIC), 190 patients, Global Initiative for Chronic Obstructive Lung Disease stage B–D, underwent bronchoscopy...
Total glycans from the cell layer and culture medium of human vascular smooth muscle cells (VSMC) that had been cultivated in presence platelet-derived growth factor (PDGF) were isolated purified by gel filtration after Pronase DNase digestion alkaliborohydride treatment. Measurements content neutral hexoses uronic acids revealed PDGF stimulates total glycan synthesis proliferating VSMC a linear fashion 24 h to 72 incubation. In contrast, fibroblasts, epithelial cells, or endothelial was not...
To investigate possible correlations between vitreous and/or serum levels of platelet-derived growth factor isoforms (PDGF-AA, -AB and -BB) with parameters associated non-proliferative diabetic retinopathy (NPDR) clinically significant macula oedema (CSMO); to compare the results relevant regarding vascular endothelial (VEGF), which is an established affecting NPDR.Fifteen patients NPDR, 31 proliferative (PDR) 15 non-diabetic were included in study. Vitreous samples obtained during...
Hypoxia is associated with extracellular matrix remodeling in several inflammatory lung diseases, such as fibrosis, chronic obstructive pulmonary disease, and asthma. In a human cell culture model, we assessed whether modification by hypoxia platelet-derived growth factor (PDGF) involves the action of metalloproteinases (MMPs) thereby affects proliferation. Expression MMP its activity were zymography enzyme-linked immunosorbent assay fibroblasts vascular smooth muscle cells (VSMCs),...
Purpose: We investigated possible correlations between vitreous and/or serum levels of platelet derived growth factor isoforms (PDGF-AA, -AB, -BB) with parameters associated proliferative diabetic retinopathy (PDR), and compared the results to vascular endothelial (VEGF), which is an established affecting PDR. Methods: Thirty-one patients PDR 15 non-diabetic were included in study. Vitreous samples obtained during vitrectomy. PDGF-AA, -BB, as well VEGF, measured by enzyme-linked...
Glycosaminoglycans (GAG) are essential extracellular matrix molecules which regulate tissue flexibility, a parameter that is reduced in airways of patients with asthma and chronic obstructive pulmonary disease (COPD). We investigated the expression GAG their metabolising enzymes primary human airway smooth muscle cells (ASMC) obtained from healthy donors (controls) or COPD. Total synthesis was assessed by [ 3 H]-glucosamine incorporation. were isolated, purified, fractionated electrophoresis...
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