A5101648442- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Renal cell carcinoma treatment
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Renal and related cancers
- Biofuel production and bioconversion
- Weed Control and Herbicide Applications
- Pesticide and Herbicide Environmental Studies
- CRISPR and Genetic Engineering
- Multiple and Secondary Primary Cancers
- MicroRNA in disease regulation
- Plant Virus Research Studies
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Time Series Analysis and Forecasting
- Animal Virus Infections Studies
- Transgenic Plants and Applications
- Metabolism, Diabetes, and Cancer
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Chromosomal and Genetic Variations
- Cellular transport and secretion
- Genetically Modified Organisms Research
Chinese Academy of Sciences
2015-2024
University of Science and Technology of China
2023-2024
Anhui Medical University
2024
Chinese Academy of Agricultural Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Institute of Crop Sciences
2024
Hefei Institutes of Physical Science
2015-2024
Center for Excellence in Molecular Plant Sciences
2023-2024
University of Chinese Academy of Sciences
2023-2024
Harbin Institute of Technology
2013-2024
Glioblastomas (GBM) comprise different subsets that exhibit marked heterogeneity and plasticity, leading to a lack of success genomic profiling in guiding the development precision medicine approaches against these tumors. Accordingly, there is an urgent need investigate regulatory mechanisms for GBM identify novel biomarkers therapeutic targets relevant context GBM-specific niches. The DHHC family proteins associated tightly with malignant progression gliomas. However, role plasticity...
To cope with environmental stimuli, plants have evolved precise regulatory mechanisms to perceive, transduce and respond abiotic stresses that can negatively affect growth development. The CBL–CIPK signaling system is a newly emerging plant-specific Ca2+-dependent network mediating stress tolerance. CBLs may sense Ca2+ signature triggered by stresses, specific interactions novel CIPK-type kinases after binding Ca2+. CBL/CIPK complexes post-translationally phosphorylate downstream target...
Regulation of lignin biosynthesis is known to occur at the level transcription factors (TFs), which R2R3-MYB family members have been proposed play a central role via AC cis-elements. Despite important roles TFs in biosynthesis, post-translational regulation these TFs, particularly their ubiquitination regulation, has not thoroughly explored.We describe discovery Populus tomentosa E2 ubiquitin-conjugating enzyme 34 (PtoUBC34), involved transactivation activity lignin-associated...
Glioblastoma multiforme (GBM) almost invariably acquires an invasive phenotype, resulting in limited therapeutic options. Protein palmitoylation markedly affects tumorigenesis and malignant progression GBM. The role of protein GBM, however, has not been systematically reported. This study aimed to investigate the effect on GBM cell survival cycle. In this study, most palmitoyltransferases were upregulated its lines, participated signaling pathways controlling Inhibition with substrate-analog...
Abstract Many cellular stresses are transduced into apoptotic signals through modification or up-regulation of the BH3-only subfamily BCL2 proteins. Through direct indirect mechanisms, these proteins activate BAK and BAX to permeabilize mitochondrial outer membrane. While BIM, PUMA, tBID have been confirmed directly its canonical BH3 binding groove, whether BMF, HRK BIK can is less clear. Here we show that BMF bind BAK. NMR studies, site-directed mutagenesis, advanced molecular dynamics...
Abstract Background Forest trees have important economic and ecological value. As a model tree, poplar has played significant role in elucidating the molecular mechanisms underlying tree biology. However, lack of mutant libraries time-consuming stable genetic transformation processes severely limit progress into functional characterization genes. A convenient fast transient method is therefore needed to enhance on genomics poplar. Methods total 11 clones were screened for amenability syringe...
Circular ssDNA can sequester inhibitory microRNAs to restore tumor suppressor gene function, thereby inhibiting progression.
The vertical segments of Populus stems are an ideal experimental system for analyzing the gene expression patterns involved in primary and secondary growth during wood formation. Suitable internal control genes indispensable to quantitative real time PCR (qRT-PCR) assays expression. In this study, stability eight candidate reference was evaluated a series stem tomentosa. Analysis through software packages geNorm, NormFinder BestKeeper showed that ribosomal protein (RP) tubulin beta (TUBB)...
Radioadaptive response (RAR) in mammalian cells refers to the phenomenon where a low-dose ionizing irradiation alters gene expression profiles, and protects from detrimental effects of subsequent high dose exposure. Despite completion numerous experimental studies on RAR, underlying mechanism has remained unclear. In this study, we aimed have comprehensive investigation RAR induced AG01522 human fibroblasts first exposed 5 cGy (priming dose) then followed by 2 Gy (challenge X-ray through...
Although research into immunotherapy is growing, its use in the treatment of breast cancer remains limited. Thus, identification and evaluation prognostic biomarkers tissue microenvironments will reveal new immune-based therapeutic strategies for cancer. Using an silico bioinformatic approach, we investigated tumor microenvironmental genetic factors related to We calculated Immune score, Stromal Estimate Tumor purity, TMB (Tumor mutation burden), MATH (Mutant-allele heterogeneity) Breast...
Precise sequence insertion or replacement in plants is technically challenging but of great importance crop breeding because many agronomic traits are affected by DNA fragment variations. Although prime editing (PE) has been continuously optimized to improve its activity (Jiang et al., 2022; Li 2022a,b; Zong 2022), it still inefficient for targeted longer sequences. Similar strategies, twinPE (Anzalone 2022) and GRAND (Wang which a pair PE guide RNAs (pegRNAs) partially complementary each...
Identification of new binding targets is essential for the development herbicides and phytotoxin-tolerant crops. Caprylic acid (CAP) a safe non-selective bio-herbicide in uncultivated areas. However, herbicidal action CAP remains unclear. Herein, metabolomic proteomic profiling indicated that serine hydroxymethyl transferase Conyza canadensis ( Cc SHMT1) promising candidate targeted CAP. The protein abundance activity SHMT1 were decreased time- dosage-dependent manners after treatment....
Identification of new binding targets is essential for the development herbicides and phytotoxin-tolerant crops. Caprylic acid (CAP) a safe non-selective bio-herbicide in uncultivated areas. However, herbicidal action CAP remains unclear. Herein, metabolomic proteomic profiling indicated that serine hydroxymethyl transferase Conyza canadensis ( Cc SHMT1) promising candidate targeted CAP. The protein abundance activity SHMT1 were decreased time- dosage-dependent manners after treatment....
Transgenic poplar lines 'Shanxin' (Populus davidiana×Populus bolleana) were generated via Agrobacterium-mediated transformation. The transgenic carried the expression cassettes of Cry1Ac + SCK, Cry1Ah3, and Cry9Aa3, respectively. levels exogenous insect resistance genes in determined by Q-PCR Western blot. Leaves used for feeding bioassays on first instar larvae gypsy moth (Lymantria dispar) fall webworm (Hyphantria cunea). At 5 d feeding, mean mortalities SCK Cry1Ah3 poplars leaves 97% 91%,...
The mechanical properties of guard cells have major effects on stomatal functioning. Reinforced stiffness in the polar regions was recently proposed to play an important role function, but underlying molecular mechanisms remain elusive. Here, we used genetic and biochemical approaches poplar (Populus spp.) show that transcription factor MYB156 controls pectic homogalacturonan-based stiffening through downregulation gene encoding pectin methylesterase 6 (PME6). Loss increased stomata, thereby...