A5066398897- Plant Molecular Biology Research
- Enzyme Catalysis and Immobilization
- Psoriasis: Treatment and Pathogenesis
- Plant Reproductive Biology
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Colorectal Cancer Surgical Treatments
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Dermatology and Skin Diseases
- Biofuel production and bioconversion
- S100 Proteins and Annexins
- Chromium effects and bioremediation
- Genetically Modified Organisms Research
- Cytokine Signaling Pathways and Interactions
- Gout, Hyperuricemia, Uric Acid
- Adipose Tissue and Metabolism
- Immune cells in cancer
- Mesenchymal stem cell research
- Colorectal and Anal Carcinomas
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
Nanjing Agricultural University
2019-2024
Ministry of Agriculture and Rural Affairs
2019-2024
Zhejiang Provincial People's Hospital
2024
Sichuan University
2024
West China Second University Hospital of Sichuan University
2024
Beijing Academy of Agricultural and Forestry Sciences
2014-2023
Jiangsu University
2021-2022
Jinan University
2017-2021
China Pharmaceutical University
2020-2021
Xuzhou Medical College
2021
Interleukin-37 (IL-37), a new member of IL-1 family cytokine, is recently identified as natural inhibitor innate immunity. This study aimed to measure the peripheral blood mononuclear cells (PBMCs) and serum levels IL-37 in patients with systemic lupus erythematosus (SLE) investigate its role SLE, including correlation disease activity, organ disorder regulation inflammatory cytokines. The expressions mRNAs PBMCs 66 SLE were measured by real-time polymerase chain reaction (RT-PCR)...
Objective Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease, and its pathogenetic mechanism far from being understood. In this study, we aimed to explore the cellular molecular mechanisms that lead pathogenesis of disease. Methods We applied single-cell RNA sequencing (scRNA-seq) 57,288 peripheral blood mononuclear cells (PBMCs) five patients with pSS healthy controls. The immune cell subsets susceptibility genes involved in were analyzed. Flow cytometry was preformed verify...
Abstract WRKY transcription factors (TFs) are of great importance in plant responses to different abiotic stresses. However, research on their roles the regulation thermotolerance remains limited. Here, we investigated function LlWRKY39 lily ( Lilium longiflorum ‘white heaven’). According multiple alignment analyses, is IId subclass and contains a potential calmodulin (CaM)-binding domain. Further analysis has shown that LlCaM3 interacts with by binding its CaM-binding domain, this...
Stem cell therapy has emerged as a promising therapeutic strategy for myocardial infarction (MI). However, the poor viability of transplanted stem cells hampers their efficacy. Hypoxic preconditioning (HPC) can effectively promote survival cells. The aim this study was to investigate whether HPC improved functional bone marrow mesenchymal (BM-MSCs) and increased cardiac protective effect.BM-MSCs, isolated from Tg(Fluc-egfp) mice which constitutively express both firefly luciferase (Fluc)...
Heat stress transcription factors (HSFs) are central regulators of plant responses to heat stress. Their heat-induced transcriptional regulation has been extensively studied; however, their posttranscriptional and posttranslational is poorly understood. In a previous study, we established that there were at least two
Abstract Introduction Our objective in the present study was to determine signaling pathway of interleukin 10 (IL-10) for modulating IL-17 expression macrophages and importance this mediation collagen-induced arthritis (CIA). Methods IL-10-knockout (IL-10 −/− ) mice wild-type (WT) were immunized with chicken type II collagen (CII) induce arthritis. The levels retinoid-related orphan receptor γt (RORγt) joint tissues IL-10 WT analyzed by enzyme-linked immunosorbent assay, quantitative RT-PCR...
Abstract Bone marrow‐derived mesenchymal stem cells (BM‐MSCs) have been demonstrated as an ideal autologous source for cell‐based therapy myocardial infarction (MI). However, poor viability of donor after transplantation limits their therapeutic efficiency, whereas the underlying mechanism is still poorly understood. Autophagy, a highly conserved process cellular degradation, required maintaining homeostasis and normal function. Here, we investigated potential role autophagy on apoptosis in...
Several recent reports have shown irisin protects the heart against ischemia/reperfusion injury. However, effect of on I/R injury in diabetic mice has not been described. The present study was designed to investigate role myocardial ischemia-reperfusion (MI/R) mice.A mouse model diabetes established by feeding wild type or gene-manipulated adult male with a high-fat diet. All received intraperitoneal injection PBS. Thirty minutes after injection, were subjected 30 min ischemia followed 3h...
Abstract It is now well accepted that an imbalance between the Th17 and regulatory T‐cell responses closely associated with development of rheumatoid arthritis (RA). However, precise mechanism for differentiation Treg in RA not characterized. The present study showed metallothionein‐1 (MT‐1), which a low molecular weight protein involved detoxification heavy metals scavenging free radicals, was upregulated RA. Furthermore, synovial inflammation pathologic symptoms collagen‐induced collagen...
Regulation of lignin biosynthesis is known to occur at the level transcription factors (TFs), which R2R3-MYB family members have been proposed play a central role via AC cis-elements. Despite important roles TFs in biosynthesis, post-translational regulation these TFs, particularly their ubiquitination regulation, has not thoroughly explored.We describe discovery Populus tomentosa E2 ubiquitin-conjugating enzyme 34 (PtoUBC34), involved transactivation activity lignin-associated...
Abstract Background Forest trees have important economic and ecological value. As a model tree, poplar has played significant role in elucidating the molecular mechanisms underlying tree biology. However, lack of mutant libraries time-consuming stable genetic transformation processes severely limit progress into functional characterization genes. A convenient fast transient method is therefore needed to enhance on genomics poplar. Methods total 11 clones were screened for amenability syringe...
Calcyclin-binding protein (CacyBP)/Siah-1 interacting (SIP), a component of ubiquitin-mediated proteolysis, could bind the Skp1-Cul1-F box complex. Although CacyBP/SIP was implicated in p53-induced β-catenin degradation, its exact function still unknown. Our previous studies showed that modulate multidrug-resistant phenotype gastric cancer cells and highly expressed tissues compared with non-cancerous tissues. In this study, expression profile broad range human normal carcinomas analyzed by...
Nebivolol, third-generation β-blocker, may activate β3-adrenergic receptor (AR), which has been emerged as a novel and potential therapeutic targets for cardiovascular diseases. However, it is not known whether nebivolol administration plays cardioprotective effect against myocardial infarction (MI) injury. Therefore, the present study was designed to clarify effects of on MI injury elucidate underlying mechanism. model constructed by left anterior descending (LAD) artery ligation. β3-AR...
Abstract The objective of this study is to investigate the correlation between IL-37 level and osteoarthritis activity determine anti-inflammatory effects in peripheral blood mononuclear cells (PBMCs) synovial (SCs) from (OA) patients, which including 32 patients with erosive inflammatory OA (EIOA) 40 primary generalized (PGOA), age sex matched healthy volunteers were recruited as controls (HCs). protein relative mRNA levels significant increased EIOA compared those PGOA HCs. Serum...
The vertical segments of Populus stems are an ideal experimental system for analyzing the gene expression patterns involved in primary and secondary growth during wood formation. Suitable internal control genes indispensable to quantitative real time PCR (qRT-PCR) assays expression. In this study, stability eight candidate reference was evaluated a series stem tomentosa. Analysis through software packages geNorm, NormFinder BestKeeper showed that ribosomal protein (RP) tubulin beta (TUBB)...
In the present study, a feruloyl esterase DLFae4 identified in our previous research was modified by error-prone PCR and site-directed saturation mutation to enhance catalytic efficiency acyltransferase activity further. Five mutants with 6.9-118.9% enhanced toward methyl ferulate (MFA) were characterized under optimum conditions. Double variant DLFae4-m5 exhibited highest hydrolytic (270.97 U/mg), Km value decreased 83.91%, Kcat/Km increased 6.08-fold MFA. Molecular docking indicated that...
This study aims to determine a role of interleukin-17A (IL-17) in salivary gland (SG) dysfunction and therapeutic effects targeting IL-17 SG for treating autoimmune sialadenitis primary Sjögren's syndrome (pSS).Salivary levels IL-17-secreting cells labial glands pSS patients were examined. Kinetic changes IL-17-producing from mice with experimental (ESS) analysed. To secretion, IL-17-deficient constructed chimeric receptor C (IL-17RC) deficiency non-hematopoietic hematopoietic examined...