A5030168441- Enzyme Catalysis and Immobilization
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Antimicrobial Peptides and Activities
- biodegradable polymer synthesis and properties
- Fungal Biology and Applications
- Chemical synthesis and alkaloids
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- Plant-Microbe Interactions and Immunity
- Cancer Treatment and Pharmacology
- Computational Drug Discovery Methods
- Plant biochemistry and biosynthesis
Nanjing Agricultural University
2023
In the present study, a feruloyl esterase DLFae4 identified in our previous research was modified by error-prone PCR and site-directed saturation mutation to enhance catalytic efficiency acyltransferase activity further. Five mutants with 6.9-118.9% enhanced toward methyl ferulate (MFA) were characterized under optimum conditions. Double variant DLFae4-m5 exhibited highest hydrolytic (270.97 U/mg), Km value decreased 83.91%, Kcat/Km increased 6.08-fold MFA. Molecular docking indicated that...
Plipastatin is a cyclic lipopeptide synthesized by non-ribosomal peptide synthetases (NRPS), which has diverse range of applications in postharvest preservation fruits and vegetables, biological control, feed processing. Whereas the yield plipastatin wild Bacillus sp. low, its chemical structure complex challenging to synthesize, significantly limiting production application. ComQXPA-PsrfA, quorum-sensing (QS) circuit from amyloliquefaciens, was constructed this study. Two QS promoters...
Futerpene, an unprecedented C22 bishomoditerpene featuring a unique C-5 ether ring, together with five known compounds, including deoxynivalenol (2), L-pyroglutamic acid (3), (R)-α-Hydroxy-1H-indole-2-propanoic (4), N2′-acetyltryptophan methyl ester (5) and cerebroside (6), were isolated from endophytic fungus Fusarium tricinctum RHM-PTF9 obtained the Salicornia bigelovii Torr. The chemical structures of these compounds elucidated by extensive spectroscopic analysis electronic circular...