A5101503265- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- Ginseng Biological Effects and Applications
- vaccines and immunoinformatics approaches
- Metabolism, Diabetes, and Cancer
- Muscle metabolism and nutrition
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Machine Learning in Bioinformatics
- Neurobiology and Insect Physiology Research
- Glycosylation and Glycoproteins Research
- Blood Coagulation and Thrombosis Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Salmonella and Campylobacter epidemiology
- Carbohydrate Chemistry and Synthesis
- Plant biochemistry and biosynthesis
- Traditional Chinese Medicine Analysis
- Polyamine Metabolism and Applications
- Peptidase Inhibition and Analysis
- Amino Acid Enzymes and Metabolism
- Protease and Inhibitor Mechanisms
Zhengzhou Fruit Research Institute
2025
Chinese Academy of Agricultural Sciences
2025
Zhengzhou University
2022-2025
China Pharmaceutical University
2021-2025
Zhejiang University
2024
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2021-2022
University of Electronic Science and Technology of China
2022
Cangzhou Central Hospital
2017-2022
First Affiliated Hospital of Henan University
2022
People's Hospital of Cangzhou
2021
The mechanistic target of rapamycin (mTOR) is a central mediator protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation human muscle the basal state as well immediately, 1 3 h after an acute bout resistance exercise fed (FED; 20 g Protein/40 carbohydrate/1 fat) or energy-free control (CON) state. lysosomal LAMP2 were highly co-localised samples. Resistance resulted rapid translocation mTOR/LAMP2...
The mTOR pathway has been implicated in immune functions; however, its role asthma is not well understood. We found that patients experiencing an attack, when compared with remission, showed significantly elevated serum activation, increased Th17 cells and IL-4, decreased Treg IFN-γ. In asthma, activation was positively correlated the loss of Th17/Treg Th1/Th2 balance. further confirmed using ovalbumin-induced asthmatic mouse model. activated mice, demonstrated by levels p-PI3K, p-Akt,...
The role of matrix metalloproteinase-2 and 9 in the metastasis development hypopharyngeal carcinoma has not been clarified.To observe relationship between metalloproteinase-2, metalloproteinase-9 metastasis, carcinoma.This study included 42 cancer patients. mRNA protein expression levels paracancerous tissues were detected by reverse transcription-polymerase chain reaction Western blot.Reverse detection showed that was expressed both pericarcinoma tissues, but almost polypoid control...
Mechanistic target of rapamycin (mTOR) resides as two complexes within skeletal muscle. mTOR complex 1 [mTORC1-regulatory associated protein (Raptor) positive] regulates muscle growth, whereas mTORC2 [rapamycin-insensitive companion (Rictor) insulin sensitivity. To examine the regulation these in human muscle, we utilized immunohistochemical analysis to study localization before and following protein-carbohydrate feeding (FED) resistance exercise plus (EXFED) a unilateral model. In basal...
Discrimination of cancer subtypes at the single-cell level is critical for early diagnosis and accurate treatment cancer. However, discrimination breast based on their metabolite information, which could provide a new perspective cellular metabolomics, still in its infancy. Herein, high-coverage single cell metabolic analysis was carried out by combining multiple microextraction with mass spectrometry (MS). About 4300 ion signals were extracted from each assigned to lipids, energy...
Red Actinidia arguta has recently become highly popular because of its red appearance resulting from anthocyanin accumulation, and gradually an important breeding direction. However, regulators involved in biosynthesis have not been fully characterized A. arguta. Here, we demonstrated that a key R2R3-MYB transcription factor, AaMYB61-like, plays crucial role biosynthesis. The RT-qPCR results revealed transient overexpression AaMYB61-like fruit at 90–100 DAFB significantly promoted...
Anomeric stereocontrol is usually one of the major issues in synthesis complex carbohydrates, particularly those involving β-configured 2,6-dideoxyglycoside and d/l-rhamnoside moieties. Herein, we report that 2-(diphenylphosphinoyl)acetyl highly effective as a remote stereodirecting group direct these challenging β-glycosides under mild conditions. A deoxy-trisaccharide mimic sugar chain landomycin E was prepared stereospecifically high yield. The synthetic potential also highlighted...
Chemotherapy resistance has long been recognized as a major obstacle to cancer treatment. Therefore, elucidating the underlying mechanisms of chemotherapy is conducive developing new strategies improve patients' response drugs.Real-time quantitative PCR (QPCR) was applied measure expression levels lncRNAs. LINC01572 down-regulated or up-regulated in GC cells transfected with either shRNA overexpression vectors. In vitro and vivo experiments were conducted investigate role autophagy-related...
Abstract Background Brain metastasis (BM) is thought to be related the mortality and poor prognosis of non-small cell lung cancer (NSCLC). Despite promising development NSCLC treatment, treatment BM still not optimistic due existence blood-brain barrier (BBB) that prevent drug penetration, as well short median survival time patients left for treatment. In this context, further quick effective pre-clinical models needed in Here, we report a model system using zebrafish promote drugs with BM....
Liver fibrosis poses a significant global health risk due to its association with hepatocellular carcinoma (HCC) and the lack of effective treatments. Thus, need discover additional novel therapeutic targets attenuate liver diseases is urgent. Leucine-rich repeat containing 1 (LRRC1) reportedly promotes HCC development. Previously, we found that LRRC1 was significantly upregulated in rat fibrotic according transcriptome sequencing data. Herein, current work, aimed explore role underlying...
Metal–organic frameworks (MOFs) are a class of porous materials with versatile properties. In this study, ZIF-8 was employed to establish two-enzyme system by encapsulating permeabilized Bacillus subtilis cells coated glucose isomerase. B. constructed introducing the shuttle plasmid PMA5 associated overexpression trehalose synthase. Using system, produced synthase and byproduct converted fructose help The decrease in production not only relieved inhibition entire reaction chain but also...
Glabridin is a bioactive isoflavan, which has wide range of biological properties and widely used in the market health products dietary supplements. However, transformation pathway glabridin vivo unclear, bioavailability controversial among different studies. Therefore, new HPLC-Q-TOF method was developed to analyze identify prototype metabolites rats. A total 63 compounds were identified, including hydroxylation, demethylation, acetylation, demethylation carboxylation, glucuronidation,...
Abstract BACKGROUND Diapause is the arrest of development insects and can be used for effective agricultural pest management strategies. Heat shock protein 70 (Hsp70) reported to up‐regulated during diapause maintain survival in some insect species. However, its regulatory mechanism unknown. RESULTS Expression hsp70 Helicoverpa armigera was found pupal brains. To elucidate molecular mechanisms , we focused our attention on transcription factor, heat factor 1 ( HSF1) . Four alternative...
Aberrant circular RNA (circRNA) expression has been extensively discovered for its involvement in both the initiation and progression of various cancers. Through screening circRNA profile, we identified a novel has_circ_0001806, which is termed as circCSPP1 liver cancer. In present study, aim to investigate role cancer.Fluorescence situ hybridization (FISH) was used detect location circCSPP1. Function studies including MTT, colony formation assay, transwell assay flow cytometry were carried...
The inhibitory effect of Tetrandrine (Tet) on rheumatoid arthritis (RA) is well established. However, its exact molecular mechanism remains unknown.RT-qPCR coupled with western blotting was employed to analyze the expression NEAT1, miR-17-5p, and STAT3 in RA tissues and/or RA-fibroblast-like synoviocytes (RA-FLS) treated 3 μmol/L Tet for 48 h. Cell Counting Kit-8 assay flow cytometry were performed assess RA-FLS proliferation apoptosis. Luciferase reporter assays used validate interactions...
Gastric cancer is the fourth most common malignancy and third leading cause of cancer‑associated mortality globally. Accumulating studies have identified involvement microRNAs in initiation progression gastric cancer. This study was aimed to investigate expression, functional roles microRNA‑454 (miR‑454) its direct target gene According results, expression level miR‑454 demonstrated be reduced tissues cell lines compared with corresponding distant non‑tumor human immortalized epithelial,...
What is the central question of study? Is Vps34 a nutrient-sensitive activator mTORC1 in human skeletal muscle? main finding and its importance? We show that altering nutrient availability, via protein-carbohydrate feeding, does not increase kinase activity muscle. Instead, feeding increased Vps34-mTORC1 co-localization parallel to activity. These findings may have important implications understanding nutrient-induced activation muscle interaction with Vps34.The Class III PI3Kinase, Vps34,...
Recently, the incidence of thyroid cancer (THCA) has been on rise. RNA binding proteins (RBPs) and their abnormal expression are closely related to emergence pathogenesis tumor diseases. In this study, we obtained gene data corresponding clinical information from TCGA database. A total 162 aberrantly expressed RBPs were obtained, comprising 92 up-regulated 70 down-regulated RBPs. Then, performed a functional enrichment analysis constructed PPI network. Through univariate Cox regression key...