A5077218668- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Sports Performance and Training
- Diet and metabolism studies
- Genetics and Physical Performance
- Metabolism, Diabetes, and Cancer
- Amino Acid Enzymes and Metabolism
- Adipose Tissue and Metabolism
- Musculoskeletal pain and rehabilitation
- Spine and Intervertebral Disc Pathology
- PI3K/AKT/mTOR signaling in cancer
- Autophagy in Disease and Therapy
- Nutrition and Health in Aging
- Cardiovascular and exercise physiology
- Neurobiology and Insect Physiology Research
- Cervical and Thoracic Myelopathy
- Protein Kinase Regulation and GTPase Signaling
- Single-cell and spatial transcriptomics
- Physical Activity and Health
- Cellular transport and secretion
- Lymphatic System and Diseases
- Antimicrobial Peptides and Activities
- Cardiovascular Effects of Exercise
- Myofascial pain diagnosis and treatment
Manchester Metropolitan University
2022-2024
University of Toronto
2019-2024
University of Birmingham
2017-2020
Henry Royce Institute
2016-2018
University of Manchester
2016-2018
The aetiology of intervertebral disc (IVD) degeneration remains poorly understood. Painful IVD is associated with an acidic intradiscal pH but the response NP cells to this aberrant microenvironmental factor be fully characterised. aim here was address hypothesis that pH, similar found in degenerate IVDs, leads altered cell/functional phenotype observed during degeneration, and investigate involvement acid-sensing ion channel (ASIC) -3 response. Human were treated a range from non-degenerate...
The mechanistic target of rapamycin (mTOR) is a central mediator protein synthesis in skeletal muscle. We utilized immunofluorescence approaches to study mTOR cellular distribution and protein-protein co-localisation human muscle the basal state as well immediately, 1 3 h after an acute bout resistance exercise fed (FED; 20 g Protein/40 carbohydrate/1 fat) or energy-free control (CON) state. lysosomal LAMP2 were highly co-localised samples. Resistance resulted rapid translocation mTOR/LAMP2...
Mechanistic target of rapamycin (mTOR) resides as two complexes within skeletal muscle. mTOR complex 1 [mTORC1-regulatory associated protein (Raptor) positive] regulates muscle growth, whereas mTORC2 [rapamycin-insensitive companion (Rictor) insulin sensitivity. To examine the regulation these in human muscle, we utilized immunohistochemical analysis to study localization before and following protein-carbohydrate feeding (FED) resistance exercise plus (EXFED) a unilateral model. In basal...
We demonstrate that training-induced increase in SC content type II fibers and myonuclear I is similar between males females. Furthermore, these changes are related to the extent of muscle fiber hypertrophy. Thus, accretion appear contribute hypertrophy irrespective sex, highlighting importance stem cells human skeletal growth.
The branch chain amino acid leucine is a potent stimulator of protein synthesis in skeletal muscle. Leucine rapidly enters the cell via L-Type Amino Acid Transporter 1 (LAT1); however, little known regarding localisation and distribution this transporter human Therefore, we applied immunofluorescence staining approaches to visualise LAT1 wild type (WT) muscle-specific knockout (mKO) mice, addition basal muscle samples. positive was visually greater WT muscles compared mKO In muscle, noted...
Resistance training induces skeletal muscle hypertrophy via the summated effects of postexercise elevations in myofibrillar protein synthesis (MyoPS) that persist for up to 48 h, although research females is currently lacking. MyoPS regulated by mTOR translocation and colocalization; however, resistance on these intracellular processes are unknown. We hypothesized would correlate with only after both sexes be associated redistribution mTOR.Recreationally active males (n = 10 each) underwent...
We recently demonstrated that acute oral ketone monoester intake induces a stimulation of postprandial myofibrillar protein synthesis rates comparable to elicited following the ingestion 10 g whey or their coingestion. The present investigation aimed determine effects ingesting monoester, protein, coingestion on mechanistic target rapamycin (mTOR)-related protein-protein colocalization and intracellular trafficking in human skeletal muscle. In randomized, double-blind, parallel group design,...
Postexercise protein ingestion can elevate rates of myofibrillar synthesis (MyoPS), mTORC1 activity, and mTOR translocation/protein-protein interactions. However, it is unclear if leucine-enriched essential amino acids (LEAA) similarly facilitate intracellular trafficking in humans after exercise. The purpose this study was to determine the effect postexercise LEAA (4 g total EAAs, 1.6 leucine) on acute MyoPS translocation signaling. Recreationally active men performed lower-body resistance...
The influx of essential amino acids into skeletal muscle is primarily mediated by the large neutral acid transporter 1 (LAT1), which dependent on glutamine gradient generated sodium-dependent 2 (SNAT2). protein expression and membrane localization LAT1 may be influenced ingestion and/or resistance exercise, although its acute influence dietary incorporation has not been investigated. In a group design, healthy males consumed mixed carbohydrate (0.75 g·kg-1) crystalline (0.25 beverage...
Mechanical stimulation is known to influence intervertebral disc (IVD) cell behavior and function, but the effect on cells routinely considered in isolation from other microenvironmental factors. Acidic pH has been shown be a prominent detrimental factor present degenerate IVDs, its human mechanoresponse never studied. We investigated response of agarose‐encapsulated nucleus pulposus (NP) 0.004 MPa, 1.0 Hz 1 hour compression (Flexcell FX4000 Compression System) under conditions...
Breaking up prolonged sitting with intermittent walking breaks can improve glycemic control. Here, we demonstrated that interrupting every 30 min 1 of repeated chair stands was as effective 2-min treadmill walks for lowering postprandial insulinemia in healthy adults. Markers contraction- and insulin-mediated muscle glucose uptake were unchanged. Repeated a form body-weight resistance activity may represent cost- space-efficient break mitigating cardiometabolic-disease risk.
Skeletal muscle repair and maintenance are directly indirectly supported by interstitial cell populations such as vascular cells fibro-adipogenic progenitors (FAPs), a subset of which express Twist2 possess direct myogenic potential. Furthermore, work in rodents has highlighted the potential pericytes to act progenitor cells, giving rise transdifferentiating into endothelial cells. However, less is understood about these human skeletal muscle. Here, we performed single-cell RNA sequencing...
Following anabolic stimuli (mechanical loading and/or amino acid provision), the mechanistic target of rapamycin complex 1 (mTORC1), a master regulator protein synthesis, translocates toward cell periphery. However, it is unknown if mTORC1-mediated phosphorylation events occur in these peripheral regions or before translocation (i.e., central regions). We therefore aimed to determine cellular location event, RPS6Ser240/244, human skeletal muscle following stimuli. Fourteen young, healthy...
Abstract Background Sarcopenia is thought to be underlined by age-associated anabolic resistance and dysregulation of intracellular signalling pathways. However, it unclear whether these phenomena are driven ageing per se or other confounding factors. Methods Lean healthy young (n=10, 22 ± 3 yrs, BMI; 23.4 0.8 kg/m 2 ) old men 70 22.7 1.3 performed unilateral exercise followed intake essential amino acids (EAA). Muscle biopsies were collected from the rested exercised leg before, immediately...
ABSTRACT Background Sarcopenia is thought to be underlined by age‐associated anabolic resistance and dysregulation of intracellular signalling pathways. However, it unclear whether these phenomena are driven ageing per se or other confounding factors. Methods Lean healthy young ( n = 10, 22 ± 3 years, BMI; 23.4 0.8 kg/m 2 ) old men 70 22.7 1.3 performed unilateral exercise followed intake essential amino acids (EAA). Muscle biopsies were collected from the rested exercised leg before,...
Interrupting prolonged sitting with intermittent exercise enhances postprandial glycemic control but has unknown effects on sensitizing skeletal muscle to dietary amino acids. We hypothesized that brief walking or body weight squats would enhance the utilization of phenylalanine for myofibrillar protein synthesis (MyoPS) during sitting. Participants (7 males and 5 females; ∼23 yr; ∼25.1 kg/m2; ∼7,300 steps/day) completed three 7.5-h trials consisting (SIT) (every 30 min) (WALK) squatting...
What is the central question of study? Is Vps34 a nutrient-sensitive activator mTORC1 in human skeletal muscle? main finding and its importance? We show that altering nutrient availability, via protein-carbohydrate feeding, does not increase kinase activity muscle. Instead, feeding increased Vps34-mTORC1 co-localization parallel to activity. These findings may have important implications understanding nutrient-induced activation muscle interaction with Vps34.The Class III PI3Kinase, Vps34,...