A5041568744- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Animal Virus Infections Studies
- X-ray Diffraction in Crystallography
- Immune Cell Function and Interaction
- Crystallization and Solubility Studies
- Mosquito-borne diseases and control
- Diet and metabolism studies
- Coastal wetland ecosystem dynamics
- Gut microbiota and health
- interferon and immune responses
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Birth, Development, and Health
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Invertebrate Immune Response Mechanisms
- Bacteriophages and microbial interactions
- Myxozoan Parasites in Aquatic Species
- Spine and Intervertebral Disc Pathology
- Genomics and Chromatin Dynamics
- Reproductive biology and impacts on aquatic species
- Plant Virus Research Studies
- Advanced Proteomics Techniques and Applications
University of Manchester
2018-2025
Norwegian Veterinary Institute
2020-2025
Wellcome Centre for Cell-Matrix Research
2025
Henry Royce Institute
2018-2025
Norwegian Institute of Marine Research
2014-2024
Manchester Academic Health Science Centre
2024
Vaxxinova (Norway)
2023
Columbia University Irving Medical Center
2021
GlaxoSmithKline (United States)
2020
Savitribai Phule Pune University
2015-2019
<h3>Background and aims:</h3> <i>Helicobacter pylori</i> infection is the major cause of peptic ulceration gastric adenocarcinoma. To address hypothesis that human acquired immune response to <i>H influences pathogenesis, we characterised T helper (Th) regulatory cell (Treg) infected patients. <h3>Methods:</h3> The CD4<sup>+</sup> 28 donors who were with 44 not was analysed using flow cytometry. associated mucosal cytokine by real-time polymerase chain reaction assay samples from 38 22...
Proteins associated with cancer cell plasma membranes are rich in known drug and antibody targets as well other proteins to play key roles the abnormal signal transduction processes required for carcinogenesis. We describe here a proteomics process that comprehensively annotates protein content of breast tumor defines clinical relevance such proteins. Tumor-derived lines were used ensure an enrichment cell-specific membrane because it is difficult purify cells then obtain good preparations...
Colloids with high-symmetry patches are functionalized metal-coordination-based recognition units and assembled into larger chain architectures, demonstrating for the first time use of metal coordination as a specific force in colloidal self-assembly. The cross-linked poly(styrene)-based patchy particles fabricated by encapsulation clusters following two-stage swelling polymerization methodology. particle patches, containing carboxylic acid groups, site-specifically either triblock copolymer...
hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that expressed in cement gland, an ectodermal organ head associated with anteroposterior fate determination during early development. Although roles mammalian cells unknown, both share a high degree protein sequence homology lie adjacent to one another on chromosome 7p21. mRNA expression has previously been demonstrated oestrogen receptor (ER)-positive cell lines. In this study,...
Interactions among host, microbiota and viral pathogens are complex poorly understood. The goal of the present study is to assess changes in skin microbial community Atlantic salmon (Salmo salar L.) response experimental infection with salmonid alphavirus (SAV). was determined using 16S rDNA pyrosequencing five different groups: control, 7 days after low-dose SAV, 14 high-dose SAV. Both treatment time were strong predictors composition. Skin samples from SAV3 infected fish showed an...
A Zika self-amplifying mRNA vaccine delivered by a flexible bedside mixing formulation induces protective immunity in NHPs.
A new class of treatments termed bioelectronic medicines are now emerging that aim to target individual nerve fibres or specific brain circuits in pathological conditions repair lost function and reinstate a healthy balance. Carotid sinus (CSN) denervation has been shown improve glucose homeostasis insulin-resistant glucose-intolerant rats; however, these positive effects from surgery appear diminish over time heavily caveated by the severe adverse associated with permanent loss chemosensory...
Real time RT-PCR has become an important tool for analyzing gene expression in fish. Although several housekeeping genes have been evaluated Atlantic halibut (Hippoglossus Hippoglossus L.), appropriate reference low copy mRNA transcripts at the earliest developmental stages not identified. No attempts reported to identify suitable infected with NNV or stimulated leucocytes. In this study, β-actin1 (ACTB1), elongation factor 1 alpha (EF1A1), hypoxanthine-guanine phosphoribosyltransferase...
Genes encoding the five Atlantic halibut (Hippoglossus hippoglossus L.) cytokines; interleukin (IL)-1β, IL-6, IL-11b, IL-12βc, and interferon (IFN) γ, were cloned characterised at a molecular level. The genomic organisation of cytokine genes was similar to that seen in mammals and/or other fish species. Several mRNA instability motifs found within 3′-untranslated region (UTR) all cDNA sequences. putative protein sequences showed low sequence identity with corresponding homologues mammals,...
Viruses are threatening pathogens for fish aquaculture. Some of them transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (NNV). In order to be the gonad, should colonize and replicate inside some cell types this tissue avoid subsequent immune response locally. However, whether NNV colonizes that infected, how in is regulated has never been studied. We have demonstrated first time presence localization into testis after an experimental infection European sea bass...
Obesity-related adipose tissue dysfunction has been linked to the development of insulin resistance, type 2 diabetes, and cardiovascular disease. Impaired calcium homeostasis is associated with altered metabolism; however, molecular mechanisms that link disrupted signaling metabolic regulation are largely unknown. Here, we investigated contribution a calcium-sensing enzyme, calcium/calmodulin-dependent protein kinase II (CAMK2), adipocyte function, obesity-associated glucose intolerance. To...
The microbial communities that live in symbiosis with the mucosal surfaces of animals provide host defense strategies against pathogens. These are largely shaped by environment and genetics. Triploid Atlantic salmon ( Salmo salar ) being considered for aquaculture as they reproductively sterile thus cannot contaminate natural gene pool. It has not been previously investigated how microbiome triploid compares to their diploid counterparts. In this study, we compare steady-state skin gill both...
ABSTRACT Aquaculture farming discharges large volumes of fish sludge, consisting particulate organic matter from feed spill and feces. Fish sludge land‐based salmonid aquaculture systems can potentially be used to low‐trophic species such as marine polychaetes insects. Further use these invertebrates may provide a more sustainable chain. However, food security must not compromise or safety. The is currently permitted in the European Union (EU). A regulatory assessment that considers...
Abstract Background Intervertebral disc (IVD) degeneration is a common cause of low back pain, and the most symptomatic patients with neural compression need surgical intervention to relieve symptoms. Current techniques used diagnose IVD degeneration, such as magnetic resonance imaging (MRI), do not detect changes in tissue extracellular matrix (ECM) progresses. Improved techniques, combination blood biomarkers, are needed monitor progression for more effective treatment plans. Methods To...
Abstract Scaffold/matrix attachment regions (S/MARs) are DNA elements that serve to compartmentalize the chromatin into structural and functional domains. These involved in control of gene expression which governs phenotype also plays role disease biology. Therefore, genome-wide understanding these holds great therapeutic promise. Several attempts have been made toward identification S/MARs genomes various organisms including human. However, a comprehensive map human is yet not available....
Pancreas disease (PD), caused by salmonid alphavirus (SAV), is an important affecting aquaculture. It has been speculated that Atlantic salmon post-smolts are more prone to infections in the first few weeks following seawater- transfer. After this period of seawater acclimatization, robust and better able resist infection pathogens. Here we describe how established a bath immersion (BI) model for SAV subtype 3 (SAV3) seawater. We also report challenge was used study susceptibility SAV3 two...
Two series of homoleptic phenolate complexes with fluorinated aryloxide ligands A2[M(OAr)4] M=Co2+ or Cu2+, OAr-=(OC6F5)- (OArF) [3,5-OC6H3(CF3)2]- (OAr'), A+=K (18-crown-6)+, Tl+, Ph4P+, Et3HN+, Me4N+ have been synthesized. related nonfluorinated phenoxide synthesized and studied in comparison to the aryloxides demonstrating dramatic structural changes effected by modification OPh OAr(F). The compounds [K(18-crown-6)]2[Cu(OArF)4], 1a; [K(18-crown-6)]2[Cu(OAr')4], 1b; [Tl2Cu(OArF)4], 2a;...