A5024278695- Pharmacological Effects of Natural Compounds
- Ginseng Biological Effects and Applications
- Traditional Chinese Medicine Analysis
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Analytical Chemistry and Chromatography
- Plant-based Medicinal Research
- Metabolomics and Mass Spectrometry Studies
- Natural product bioactivities and synthesis
- Analytical Methods in Pharmaceuticals
- Cancer therapeutics and mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Berberine and alkaloids research
- Phytochemistry and Biological Activities
- Drug-Induced Hepatotoxicity and Protection
- HIV/AIDS drug development and treatment
- Cancer, Hypoxia, and Metabolism
- Cancer Treatment and Pharmacology
- Inflammatory mediators and NSAID effects
- Bioactive natural compounds
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Ginkgo biloba and Cashew Applications
State Key Laboratory of Natural Medicine
2014-2025
China Pharmaceutical University
2016-2025
First Hospital of Shanxi Medical University
2024
Shanxi Medical University
2024
Yunnan University of Traditional Chinese Medicine
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2024
Second Military Medical University
2024
Army Medical University
2007-2023
Xinqiao Hospital
2007-2023
Zhejiang University
2009-2021
The objective of this study was to determine the incidence DNA fragmentation in human sperm, and correlate any detected damage with semen analysis parameters fertilization rates vitro (IVF). A total 298 samples were collected from men infertility program at Toronto Hospital. For each sample, percentage sperm determined using method terminal deoxynucleotidyl transferase-mediated dUTP-biotin end-labeling (TUNEL) fluorescence-activated cell sorting. fragmented less than 4% majority but ranged...
The objective of this study was to evaluate the effect generation reactive oxygen species (ROS) on integrity DNA human spermatozoa, and determine if pretreatment with antioxidants can reduce damage. Samples were obtained from 47 men undergoing infertility investigation. ROS generated in samples by addition xanthine/xanthine oxidase (X/XO) or without antioxidants. After incubation at timed intervals (0-2 h) X/XO, percentage spermatozoa fragmentation determined using method TdT-mediated...
Objective: To determine the incidence of DNA fragmentation in human sperm used for intracytoplasmic injection (ICSI) and to correlate any detected damage with semen analysis parameters fertilization rates ICSI.Design: Descriptive correlational clinical study.Setting: Tertiary care fertility clinic.Patient(s): A total 150 samples was collected from men ICSI program.Intervention(s): For each sample, wash swim-up were performed, percentage recovered determined use terminal transferase-mediated...
<i>Background:</i> Imperatorin (IM) is a furanocoumarin isolated from the root of <i>Angelica dahurica,</i> which reported to have anticonvulsant and anticancer effects. In this study, antiproliferative effect IM on 9 human cancer cell lines was examined, hepatoma HepG2 cells were chosen as target for preferential killing by IM. Particularly, mechanism IM-induced apoptosis in vivo animal effects also studied. <i>Methods:</i> Cell viability measured using...
Kaempferol is a biologically active component present in various plants. The hepatoprotective effect of kaempferol drug-induced liver injury has been proven, while its against alcoholic (ALI) remains unclear. Hence, the study aimed to evaluate ALI mice. experimental mice model was developed and were treated with different doses for 4 weeks. functions observed by monitoring following parameters: Aspartate aminotransferase (AST/GOT) alanine (ALT/GPT) levels serum; histopathological studies...
Abstract Acute ischemic stroke (AIS) is a global cerebrovascular disease with high disability and mortality, which has no effective therapy. Studies have demonstrated that astrocyte-derived exosomes (ADEXs) provided neuroprotection in experimental models. Nevertheless, the role of derived from oxygen-glucose-deprivation/reoxygenation-stimulated astrocytes (OGD/R-stimulated astrocytes; OGD/R-ADEXs) AIS remains largely unknown. Here, we found OGD/R-ADEXs significantly reduced OGD/R-induced...
Our previous studies have shown that berberine can improve the nerve function deficits in ischemic stroke by inhibiting inflammation. The cellular communication between astrocytes and neurons via exosomes might affect neurological after stroke, which plays a vital role therapy of stroke.The present study focused on effects released from induced glucose oxygen deprivation model with pretreatment (BBR-exos) treatment for its regulatory mechanism.Oxygen-glucose-deprivation/Reoxygenation...
Ischemic stroke (IS) is one of the principal causes disability and death worldwide. Berberine (BBR), derived from traditional Chinese herbal medicine Huang Lian, has been reported to inhibit progression stroke, but specific mechanism whereby BBR modulates ischemic remains unclear. N6-methyladenosine (m6A) modification most typical epigenetic mRNA post-transcriptional modifications, among which METTL3 common methylation transferase. During study, middle cerebral artery occlusion/reperfusion...
The mechanism of action berberine as an antihyperglycaemic agent was investigated in the Caco-2 cell line. Berberine found to effectively inhibit activity disaccharidases cells. It also decreased sucrase after preincubation with cells for 72 hours. However gluconeogenesis and glucose consumption were not influenced. 2-Deoxyglucose transporting through monolayers by but effect statistically significant. These results suggest that is at least partly due its ability alpha-glucosidase decrease...
LC/ESI/MS method was employed for the pharmacokinetic evaluation of total panax notoginsenoside (TPNS) in rats. After oral or intravenous administration TPNS at dosage 300.0 10.0 mg kg−1 to rats respectively, R1, ginsenoside Rg1, Rd, Re and Rb1 were simultaneous determined rat plasma. Pharmacokinetic parameters absolute bioavailability obtained by Drug And Statistics windows (DAS) software. The all analytes different form each other. T1/2 changed from 0.72 22.16 h AUC 1.03 98.94 mg/l·h after...
Strength-endurance type of sport can lead to modification human beings' physiological status. The present study aimed investigate the alteration metabolic phenotype or biochemical compositions in professional athletes induced by long-term training means a novel systematic tool, metabolomics. Resting venous blood samples junior and senior male rowers were obtained before after 1-wk 2-wk training. Venous from healthy volunteers as control was also sampled at rest. Endogenous metabolites serum...
The potential for herb-drug interactions has recently received greater attention worldwide, considering the fact that use of herbal products becomes more and widespread. goal this work was to examine metabolism-based drug interaction arising from seven active components (danshensu, protocatechuic aldehyde, acid, salvianolic acid B, tanshinone I, IIA, cryptotanshinone) danshen extract. Probe substrates cytochrome P450 enzymes were incubated in human liver microsomes (HLMs) with or without...
P-glycoprotein (P-gp) is an ATP-dependent efflux transporter highly expressed in gastrointestinal tract and multidrug resistance tumor cells. Inhibition or induction of P-gp can cause drug-drug interactions thus influence the effects substrate drugs. Previous studies indicated that 20(<i>S</i>)-ginsenoside Rh2 [20(<i>S</i>)-Rh2] could synergistically enhance anticancer conventional chemotherapeutic agents at a nontoxic dose. The aim present study was to investigate vitro vivo whether...
Intracellular pharmacokinetics of anticancer drugs in multi-drug resistance (MDR) cancer cells is hugely important the evaluation and improvement drug efficacy. By using adriamycin as a probe MDR cells, we developed cellular pharmacokinetic-pharmacodynamic (PK-PD) model to reveal correlation between pharmacokinetic properties resistance. In addition, ability 20(S)-ginsenoside Rh2 (20(S)-Rh2) reverse was further investigated.The were analysed visually quantitatively human breast MCF-7...
HIV patients on combination oral drug therapy experience insufficient levels in lymph nodes, which is linked to viral persistence. Following success enhancing node and extending plasma residence time of indinavir formulated lipid nanoparticles, we developed multidrug anti-HIV nanoparticles (anti-HIV LNPs) containing lopinavir (LPV), ritonavir (RTV), tenofovir (PMPA). These LNPs were prepared, characterized, scaled up, evaluated primates with a focus course intracellular exposure blood nodes....
The signaling pathways by which cell volume regulates ion transporters, e.g.Na+/H+ exchangers (NHEs), and affects cytoskeletal organization are poorly understood. We have previously shown that shrinkage induces tyrosine phosphorylation in CHO cells, predominantly an 85-kDa band. To identify volume-sensitive kinases their substrates, we investigated the effect of hypertonicity on members Src kinase family. Hyperosmolarity stimulated Fyn inhibited Src. activation was also observed...
Triptolide is a potential anti-immune agent, and has shown multi-organic toxicity, however its toxic mechanism remained undiscovered. This paper aimed at characterizing the pharmacokinetic profiles of triptolide in rats to provide clue approach mechanism. The absorption, distribution, metabolism excretion were investigated male Sprague-Dowley after single doses oral i.v. administration. After administration 0.6, 1.2 2.4 mg/kg, concentration plasma reached maximum within 15 min, declined...
Insufficient HIV drug levels in lymph nodes have been linked to viral persistence. To overcome lymphatic insufficiency, we developed and evaluated primates a lipid-drug nanoparticle containing lopinavir, ritonavir, tenofovir. These nanoparticles produced over 50-fold higher intracellular ritonavir tenofovir concentrations compared free drug. Plasma blood were enhanced sustained for 7 days after single subcutaneous dose, exceeding that achievable with current oral therapy.