A5079439134- Metabolomics and Mass Spectrometry Studies
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Analytical Chemistry and Chromatography
- Autophagy in Disease and Therapy
- Antibiotics Pharmacokinetics and Efficacy
- Fungal Biology and Applications
- Traditional Chinese Medicine Analysis
- Cancer-related molecular mechanisms research
- Bioactive Compounds and Antitumor Agents
- MicroRNA in disease regulation
- Ginseng Biological Effects and Applications
- Phytochemistry and biological activity of medicinal plants
- Intracerebral and Subarachnoid Hemorrhage Research
- Circular RNAs in diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Lung Cancer Treatments and Mutations
- TGF-β signaling in diseases
- Essential Oils and Antimicrobial Activity
- Natural product bioactivities and synthesis
- PARP inhibition in cancer therapy
- Acute Ischemic Stroke Management
- Venous Thromboembolism Diagnosis and Management
- Computational Drug Discovery Methods
Chinese PLA General Hospital
2013-2024
Fujian Agriculture and Forestry University
2024
ZheJiang Institute For Food and Drug Control
2009-2024
National Medical Products Administration
2021-2024
Southwest Forestry University
2023-2024
Henan University of Technology
2024
Zhejiang University of Technology
2024
University of British Columbia
2009-2023
Guangxi Medical University
2023
British Columbia Children's Hospital
2018-2023
Adoptive immunotherapy with regulatory T cells (Tregs) is a promising treatment for allograft rejection and graft-versus-host disease (GVHD). Emerging data indicate that, compared polyclonal Tregs, disease-relevant antigen-specific Tregs may have numerous advantages, such as need fewer reduced risk of nonspecific immune suppression. Current methods to generate alloantigen-specific rely on expansion allogeneic antigen-presenting cells, which requires access donor recipient multiple MHC...
Our previous work has shown that autophagy plays a pro-survival function in two necrotic cell death models: zVAD-treated L929 cells as well H(2)O(2)-treated Bax(-/-)Bak(-/-) mouse embryonic fibroblasts (DKO MEF). This study aims to further explore the regulatory role of necrosis by examining functional phosphoinositide-3 kinase (PI3K)-Akt-mammalian target rapamycin (mTOR) signaling pathway. initial intriguing finding was insulin is able promote induced zVAD and MNNG or H(2)O(2) DKO MEF...
Abstract Background LINE-1s (L1, Long Interspersed Element-1) are the most abundant autonomous non-LTR retrotransposons in human genome and replicate by reverse transcription of an RNA intermediate. Full-length L1 encodes two open reading frames (ORF1, ORF2) ORF2 has transcriptase activity. Results Here we expressed RT E. coli purified protein displayed same activity as that ORF2p insect cells. We tested effect different inhibitors on found all four nucleoside efficiently inhibited...
Comparison of 10 co-receptor variant CARs revealed a requirement for CD28wt optimal suppression by human regulatory T cells.
Chimeric antigen receptor (CAR) technology can be used to engineer the specificity of regulatory T cells (Tregs) and improve their potency as an adoptive cell therapy in multiple disease models. As synthetic receptors, CARs carry risk immunogenicity, particularly when derived from nonhuman antibodies. Using HLA-A*02:01–specific CAR (A2-CAR) encoding a single-chain variable fragment (Fv) mouse antibody, we developed panel 20 humanized A2-CARs (hA2-CARs). Systematic testing demonstrated...
Regulatory T cell (Treg) therapy is a promising approach to improve outcomes in transplantation and autoimmunity. In conventional therapy, chronic stimulation can result poor vivo function, phenomenon termed exhaustion. Whether or not Tregs are also susceptible exhaustion, if so, this would limit their therapeutic effect, was unknown. To "benchmark" exhaustion human Tregs, we used method known induce cells: expression of tonic-signaling chimeric antigen receptor (TS-CAR). We found that...
Abstract Cell adhesion and spreading is a crucial step in the metastatic cascade of cancer cells, interruption this considered to be logical strategy for prevention treatment tumor metastasis. Emodin major active component rhizome Rheum palmatum L., with known anticancer activities. Here, we first found that emodin significantly inhibited cell various human cells. This inhibition was achieved through suppressing recruitment focal kinase (FAK) integrin β1 as well phosphorylation FAK followed...
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at glance that was extracted from about 200 leading journals. To access Abstract, please click on HTML or PDF.
Strength-endurance type of sport can lead to modification human beings' physiological status. The present study aimed investigate the alteration metabolic phenotype or biochemical compositions in professional athletes induced by long-term training means a novel systematic tool, metabolomics. Resting venous blood samples junior and senior male rowers were obtained before after 1-wk 2-wk training. Venous from healthy volunteers as control was also sampled at rest. Endogenous metabolites serum...
Poly(ADP-ribose) polymerase-1 (PARP-1), activated by DNA strand breaks, participates in the repair process physiologically. Excessive activation of PARP-1 mediates necrotic cell death under status oxidative stress and damage. However, it remains elusive whether how is involved autophagy what function PARP-1-mediated We recently demonstrate that hydrogen peroxide (H2O2) induces through a novel signalling mechanism linking to LKB1-AMP-activated protein kinase (AMPK)-mammalian target rapamycin...
Lactate accumulation has been observed in the brain with intracerebral hemorrhage (ICH). However, outcome of lactate not well characterized. Here, we report that contributes to angiogenesis and neurogenesis ICH. In first set experiment, a rat model ICH was induced by injecting collagenase into brain. The effects on neurological function, apoptosis, numbers newborn endothelial cells neurons, as proliferation-associated signaling pathway, were evaluated second set, exogenous l-lactate infused...
Clear cell renal carcinoma (ccRCC) is the most common tumor of kidney.in adults. The prognosis ccRCC remains unsatisfactory. Recently, T immunoglobulin mucin-3 (TIM-3), a novel transmembrane protein, has been implicated in biology. Its role unknown. aim this study was to investigate roles TIM-3 as prognostic marker patients with ccRCC.TIM-3 protein expression determined by immunohistochemistry and Western blot from 137 samples adjacent normal tissues. We performed also proliferation assay...
Myocardial infarction is characterized by cardiomyocyte death and mitochondrial dysfunction induced ischemia. Ferroptosis, a novel form of cell death, has been found to play critical roles under ischemic conditions. Recently, several studies have shown that fibronectin type III domain-containing 5 (FNDC5) its cleaved form, irisin, protect the heart against injury. However, protective effect on ferroptosis impairments still unclear. Thus, our aim was investigate role irisin in cardiomyocytes...
Abstract Background Despite the popularity of modern medicine, medicinal plants remain a cornerstone treatment for numerous diseases, particularly among ethnic groups and tribal communities around globe. Ethnomedicine offers advantages such as ease use, convenience, economic benefits. Medicinal plant knowledge within Bulang community southwest China is valuable complement to Chinese ethnomedicine systems. Accumulated medical due extensive length occupation by People, considered earliest...
Background Endothelial DLL4 plays an important role in controlling of tumor angiogenesis, which is required for invasive growth and metastasis. However, the regulation clear cell renal carcinoma (ccRCC) has not yet been systematically elucidated. Methodology We performed bioinformatical analysis to explore miRNAs targeting DLL4. miR-30a was selected as a representative validate its functional association endothelial cell. Then, expressions mature from 90 cases ccRCC 28 nonmatched adjacent...
To compare and calculate the 3-year refractive results, higher-order aberrations (HOAs), contrast sensitivity (CS) dry eye parameters after small incision lenticule extraction (SMILE) wavefront-guided femtosecond laser-assisted laser in situ keratomileusis (FS-LASIK) for correction of high myopia myopic astigmatism.In this prospective, non-randomized comparative study, 78 eyes with spherical equivalent (SE) -8.11±1.09 diopters (D) received a SMILE surgery, 65 SE -8.05±1.12 D FS-LASIK surgery...