A5026700190- Protein Tyrosine Phosphatases
- Autophagy in Disease and Therapy
- Cardiomyopathy and Myosin Studies
- HER2/EGFR in Cancer Research
- Immune Cell Function and Interaction
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Nuclear Structure and Function
- Cytokine Signaling Pathways and Interactions
- Diabetes and associated disorders
- Extracellular vesicles in disease
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
Mayo Clinic
2023-2025
Mayo Clinic in Arizona
2025
WinnMed
2023
During infection and cancer, mTORC1-mediated metabolic regulation impacts CD8+ T cell effector expansion memory development. However, the mechanisms by which cells regulate mTORC1 to support their unique requirements remain unknown. Here we show that NKG7, a lysosomal protein whose expression is restricted cytotoxic lymphocytes, negatively regulates recruitment activation inhibiting assembly function of proton pump, vacuolar ATPase (v-ATPase). Human mouse lacking NKG7 more acidic lysosomes...
ABSTRACT His domain protein tyrosine phosphatase (HD-PTP; also known as PTPN23) facilitates function of the endosomal sorting complexes required for transport (ESCRTs) during multivesicular body (MVB) formation. To uncover its role in physiological homeostasis, embryonic lethality caused by a complete lack HD-PTP was bypassed through generation hypomorphic mice expressing reduced protein, resulting animals that are viable into adulthood. These exhibited marked lipodystrophy and decreased...
Abstract Endosomal Sorting Complexes Required for Transport (ESCRTs) drive reverse topology membrane remodeling events including the formation of intralumenal vesicles within multivesicular bodies, budding retroviruses from plasma membrane, and scission cytokinetic bridge. It has been difficult to study physiological relevance this machinery in mammals because many contributing components are essential viability. To bypass problem we used combinations knockout (−), hypomorphic (H) wildtype...