A5036843005- Advanced Biosensing Techniques and Applications
- Osteoarthritis Treatment and Mechanisms
- Developmental Biology and Gene Regulation
- Advanced biosensing and bioanalysis techniques
- Congenital heart defects research
- Gene expression and cancer classification
- Zebrafish Biomedical Research Applications
- Electrospun Nanofibers in Biomedical Applications
- Engineering Technology and Methodologies
- Connective tissue disorders research
- Microfluidic and Bio-sensing Technologies
- Robotic Mechanisms and Dynamics
- Single-cell and spatial transcriptomics
- Machine Learning in Bioinformatics
- Advanced Proteomics Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- Advanced Numerical Analysis Techniques
- Urticaria and Related Conditions
- Mesenchymal stem cell research
- Vasculitis and related conditions
- Pathogenesis and Treatment of Hiccups
- Cancer Cells and Metastasis
Columbia University
2021-2024
New York Proton Center
2024
Columbia University Irving Medical Center
2021
Johns Hopkins University
2007-2011
Johns Hopkins Medicine
2007-2009
Specific antimicrobial antibodies present in the sera of patients with inflammatory bowel disease (IBD) have been proven to be valuable serological biomarkers for diagnosis/prognosis disease. Herein we describe use a whole Escherichia coli proteome microarray as novel high throughput proteomics approach screen and identify new IBD. Each protein array, which contains 4,256 E. K12 proteins, was screened using individual serum from healthy controls (n = 39) clinically well characterized IBD (66...
The poor intrinsic repair capacity of mammalian joint cartilage likely contributes to the high incidence arthritis worldwide. Adult zebrafish can regenerate many structures that show limited or no healing in mammals, including jawbone. To test whether also damaged joints, we developed a surgical injury model which jaw is destabilized via transection major ligament, interopercular–mandibular (IOM). Unilateral IOM ligament 1-year-old fish resulted an initial reduction by 14 days, with full...
Abstract Objective Weight gain is a side effect of glucocorticoid (GC) use, but the natural history and health implications changes in weight that occur during treatment inflammatory disease are not understood. Methods We evaluated data from Wegener's Granulomatosis Etanercept Trial. Patients were categorized according to clinical outcome at 1 year: remission (no flares), single flare, or multiple flares. Risk factors for gaining ≥10 kg examined multivariate models. Results Weights baseline...
Abstract After traumatic injury, healing of mammalian ligaments is typically associated with fibrotic scarring as opposed to scar-free regeneration. In contrast, here we show that the ligament supporting jaw joint adult zebrafish capable rapid and complete healing. Following surgical transection ligament, observe breakdown tissue adjacent cut sites, expansion mesenchymal within wound site, then remodeling extracellular matrix (ECM) a normal morphology. Lineage tracing mature ligamentocytes...
Abstract We introduce a novel experimental methodology for the reverse‐phase protein microarray platform which reduces typical measurement CV as much 70%. The methodology, referred to array microenvironment normalization, increases statistical power of platform. In experiment, it enabled detection 1.1‐fold shift in prostate specific antigen concentration using approximately six technical replicates rather than 37 previously required. improved reproducibility and should facilitate clinical...
This study assesses the ability of a novel family machine learning algorithms to identify changes in relative protein expression levels, measured using 2-D DIGE data, which support accurate class prediction. The analysis was done training set 36 total cellular lysates comprised six normal and three cancer biological replicates (the remaining are technical replicates) validation four two samples. Protein samples were separated by quantified DeCyder-2D Differential Analysis Software. reversal...
Abstract Adult mammalian synovial joints have limited regenerative capacity, where injuries heal with mechanically inferior fibrotic tissues. Here we developed a unilateral whole-joint resection model in adult zebrafish to advance our understanding of how stimulate regrowth native joint Using combination microCT, histological, live imaging, and single-cell RNA sequencing (scRNAseq) approaches after complete removal all tissues, find de novo regeneration articular cartilage, ligament,...
The expression level of proteins governing the electrical excitability and conduction within ventricular myocardium are known to vary as a function distance through heart wall. patterns subset these altered in disease. Precise measurement such is therefore essential understanding structure-function relationships health Here, we report new experimental approach using reverse-phase protein microarrays map left transmural proteome. This can yield submillimeter spatial resolution, when coupled...
After traumatic injury, healing of mammalian ligaments is typically associated with fibrotic scarring as opposed to scar-free regeneration. In contrast, here we show that the ligament supporting jaw joint adult zebrafish capable rapid and complete healing. Following surgical transection ligament, observe breakdown tissue adjacent cut sites, expansion mesenchymal within wound site, then remodeling extracellular matrix (ECM) a normal morphology. Lineage tracing mature ligamentocytes following...