A5004131827- Viral Infections and Immunology Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- Atherosclerosis and Cardiovascular Diseases
- Eosinophilic Disorders and Syndromes
- Immunotherapy and Immune Responses
- Cardiac electrophysiology and arrhythmias
- interferon and immune responses
- Systemic Lupus Erythematosus Research
- Heart Rate Variability and Autonomic Control
- Cardiovascular Effects of Exercise
- Immune Response and Inflammation
- Advanced Thermodynamics and Statistical Mechanics
- Whipple's Disease and Interleukins
- Cardiomyopathy and Myosin Studies
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- RNA modifications and cancer
- Cardiac Fibrosis and Remodeling
- Monoclonal and Polyclonal Antibodies Research
- Dermatological and COVID-19 studies
- Economic Issues in Ukraine
- Xenotransplantation and immune response
- Inflammatory Myopathies and Dermatomyositis
Engelhardt Institute of Molecular Biology
2014-2023
The Russian Presidential Academy of National Economy and Public Administration
2022
Russian Academy of Sciences
2006-2019
Johns Hopkins University
1998-2017
University of Ottawa
2014-2015
Republican Clinical Hospital of the Ministry of Health of the Republic of Tatarstan
2013
Institute of Physiologically Active Compounds
2012
University of Calgary
2004-2008
Johns Hopkins Medicine
2005
University of Michigan
2001
Background — Interleukin (IL)-12 exerts a potent proinflammatory effect by stimulating T-helper (Th) 1 responses. This is believed to be mediated primarily through the activation of STAT4 and subsequent production interferon (IFN)-γ. Methods Results We examined role IL-12 receptor (IL-12R) signaling in development murine experimental autoimmune myocarditis (EAM) induced cardiac myosin immunization. Both IL-12Rβ1–deficient mice STAT4-deficient were resistant induction myocarditis. Treatment...
Elevated expression of chemokine receptors in tumors has been reported many instances and is related to a number survival advantages for tumor cells including abnormal activation prosurvival intracellular pathways. In this work we demonstrated an inverse correlation between levels p53 suppressor CXCR5 receptor MCF-7 human breast cancer cell line. Lentiviral transduction with shRNA led elevated at both mRNA protein levels. Functional activity p53-knockdown was also increased as shown by...
This chapter describes four murine models of autoimmune diseases: two related to myocarditis and thyroiditis. The first model, Coxsackie virus B3 (CB3)-induced myocarditis, results in the development acute susceptible as well resistant mouse strains, whereas chronic develops only genetically mice. CB3-induced closely resembles course human which is believed be initiated by viral infection. Mouse cardiac myosin heavy chain has been identified major antigen associated with late phase...
Inflammation has been recognized increasingly as a critical pathologic component of number heart diseases. A mouse model autoimmune myocarditis was developed to study the role immune mediators in development cardiac dysfunction. We have found previously that IFN-γ deficiency promotes inflammation murine myocarditis. It unclear, however, how affects function and what underlying mechanisms are responsible for these effects. In this work, we show knockout (KO) mice more pronounced systolic...
Appropriate treatment of autoimmune myocarditis following virus infection remains a major clinical problem. Induction nasal tolerance may provide new approach to treatment. However, the exact mechanism is unknown. To assess tolerance, we examined role IL-10 in induction and suppression myocarditis. First showed that blocking concurrent with administration Ag abolished disease-suppressing effect tolerization. It also led increased cardiac myosin-specific IL-1 TNF-alpha production. Then...
Global assessment of both cardiac and arterial function is important for a meaningful interpretation pathophysiological changes in animal models cardiovascular disease. We simultaneously acquired left ventricular (LV) aortic pressure LV volume (V(LV)) 17 open-chest anesthetized mice (26.7 +/- 3.2g) during steady-state (BL) caval vein occlusion (VCO) using 1.4-Fr dual-pressure conductance catheter subgroup eight animals (AOO). Aortic flow was obtained from numerical differentiation V(LV). AOO...
tThe linear time-varying elastance theory is frequently used to describe the change in ventricular stiffness during cardiac cycle. The concept assumes that all isochrones (i.e., curves connect pressure-volume data occurring at same time) are and have a common volume intercept. Of specific interest steepest isochrone, end-systolic relationship (ESPVR), of which slope serves as an index for contractile function. Pressure-volume measurements, achieved with combined pressure-conductance catheter...
Previously we showed that autoimmune myocarditis could be induced in mice by immunization with purified murine cardiac myosin (MCM). In this study, found identical disease also genetically susceptible porcine (PCM). The lesions both antigens were characterized extensive infiltration of the myocardium accompanied myocyte necrosis. A novel finding was presence multinucleated giant cells and eosinophils infiltrates, addition to a mixture mononuclear polymorphonuclear described previously....
Constrictive pericarditis represents a serious hemodynamic syndrome that may lead to heart failure. Studies of its pathophysiological mechanisms have been impeded by the lack an animal model.Cardiac myosin-induced experimental autoimmune myocarditis in interferon (IFN)-gamma-knockout (KO) mice results increased cardiac inflammation and development severe grossly detectable pericarditis. Using vivo pressure-volume studies, we found acute phase IFN-gamma-KO was characterized reduced left...
Genome-wide association studies (GWASes) revealed several single-nucleotide polymorphisms (SNPs) in the human 17q12-21 locus associated with autoimmune diseases. However, follow-up are still needed to identify causative SNPs directly mediating risk locus. We have chosen six high linkage disequilibrium GWAS hits that showed strongest evidence of causality according pattern and epigenetic data assessed their functionality a local genomic context using luciferase reporter system. found...
Abstract The role of IFN-γ in the pathogenesis autoimmune disease is controversial, being described as immunostimulatory some studies and immunosuppressive others. To determine contribution local expression IFN-γ, we derived NOD.H-2h4 transgenic mice overexpressing a thyroid-restricted manner. Transgenic mice, which had serum levels similar to wild-type littermates, showed up-regulation MHC class II on thyrocytes, but did not develop spontaneous thyroiditis. Upon immunization with murine...
Summary Complement receptors type 1 and 2 (CR1 (CD35)/CR2 (CD21)) are known to enhance the adaptive immune response. In mice, CR1/CR2 expressed on B cells, follicular dendritic activated granulocytes. Recently, we showed that a subset of CD44 high CD62L low T cells also expresses CR1 CR2. We now report detectable both CD4 + CD8 subsets cells. Lipopolysaccharide (LPS) from Gram‐negative bacteria causes polyclonal activation stimulation macrophages other antigen‐presenting further demonstrate...
Clusterin is a multifunctional protein that participates in tissue remodeling, apoptosis, lipid transport, complement-mediated cell lysis and serves as an extracellular chaperone. The role of clusterin cancer neurodegeneration has been extensively studied, however little known about its functions the immune system. Using expression profiling we found mRNA considerably down-regulated mouse spleen stroma upon knock-out lymphotoxin β receptor which plays pivotal secondary lymphoid organ...
Non-Small Cell Lung Cancer (NSCLC) is responsible for the majority of deaths caused by cancer. Small C-terminal domain (CTD) phosphatases (SCP), CTDSP1, CTDSP2 and CTDSPL (CTDSPs) belong to SCP/CTDSP subfamily are involved in many vital cellular processes tumorigenesis. High similarity their structures suggests similar functions. However role NSCLC remains insufficiently understood. For first time we revealed suppressor function CTDSPs leading a significant growth slowdown senescence A549...