A5081191206- Virus-based gene therapy research
- Cancer Research and Treatments
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Cancer, Hypoxia, and Metabolism
- Wheat and Barley Genetics and Pathology
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- interferon and immune responses
- Bacteriophages and microbial interactions
- Analog and Mixed-Signal Circuit Design
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Sensor Technology and Measurement Systems
- Biochemical and Molecular Research
- Immune Cell Function and Interaction
- Advancements in Semiconductor Devices and Circuit Design
- Agricultural Productivity and Crop Improvement
- Viral Infections and Outbreaks Research
- Chemokine receptors and signaling
Bauman Moscow State Technical University
2025
Engelhardt Institute of Molecular Biology
2015-2024
Moscow Institute of Physics and Technology
2023
Research Institute of Molecular Electronics
2021
Samara Federal Research Scientific Center
2013
Cleveland Clinic
2008
Identification of unique features cancer cells is important for defining specific and efficient therapeutic targets. Mutant p53 present in nearly half all cases, forming a promising target pharmacological reactivation. In addition to being defective the tumor-suppressor function, mutant contributes malignancy by blocking family member p73. Here, we describe small-molecule RETRA that activates set p53-regulated genes specifically suppresses p53-bearing tumor vitro mouse xenografts. Although...
Colorectal cancer (CRC) is one of the most common malignant tumors worldwide. CRC molecular pathogenesis heterogeneous and may be followed by mutations in oncogenes tumor suppressor genes, chromosomal microsatellite instability, alternative splicing alterations, hypermethylation CpG islands, oxidative stress, impairment different signaling pathways energy metabolism. In present work, we have studied alterations patterns genes related to metabolism CRC. Using CrossHub software, analyzed The...
Elevated expression of chemokine receptors in tumors has been reported many instances and is related to a number survival advantages for tumor cells including abnormal activation prosurvival intracellular pathways. In this work we demonstrated an inverse correlation between levels p53 suppressor CXCR5 receptor MCF-7 human breast cancer cell line. Lentiviral transduction with shRNA led elevated at both mRNA protein levels. Functional activity p53-knockdown was also increased as shown by...
The paper solves the task of obtaining technological conversion - three-component system ‘microdispersed mineral grain microdispersed particle viscous bitumen water’ at consecutive dosing raw material components asphalt-concrete plant. It is necessary to prepare suspension consistency avoid delamination slurry, ensure its transportation by dump trucks, storage in excavation and loading. authors propose refuse heat winter use ready-made materials for road construction cold form. addition will...
Oncolytic viruses have gained momentum in the last decades as a promising tool for cancer treatment. Despite progress, only fraction of patients show positive response to viral therapy. One key variable factors contributing therapy outcomes is interferon-dependent antiviral mechanisms tumor cells. Here, we evaluated this factor using patient-derived glioblastoma multiforme (GBM) cultures. Cell type I interferons' (IFNs) stimulation was characterized at mRNA and protein levels. Omics analysis...
Oncolytic viral therapy is a promising novel approach to cancer treatment. viruses cause tumor regression through direct cytolysis on the one hand and recruiting activating immune cells other. In this study, enhance antitumor efficacy of thymidine kinase-deficient vaccinia virus (VV, Lister strain), recombinant variants encoding bacterial flagellin (subunit B) Vibrio vulnificus (LIVP-FlaB-RFP), firefly luciferase (LIVP-Fluc-RFP) or red fluorescent protein (LIVP-RFP) were developed. The...
We developed recombinant variants of oncolytic vaccinia virus LIVP strain expressing interleukin-15 (IL-15) or its receptor subunit alpha (IL-15Rα) to stimulate IL-15-dependent immune cells. evaluated their activity either alone in combination with each other vitro and vivo using the murine CT26 colon carcinoma 4T1 breast models. demonstrated that admixture these could promote generation IL-15/IL-15Rα complex. In studies indicated cancer cells were more susceptible viruses. showed...
Glioblastoma multiforme (GBM) is one of the most common types brain tumor. Despite intensive research, patients with GBM have a poor prognosis due to very high rate relapse and significant side effects treatment, median survival 14.6 months. Oncolytic viruses are considered promising strategy eliminate other cancer, several already been introduced into clinical practice. However, identification factors that underly sensitivity tumor species oncolytic or modulate their efficacy remains an...
Among oncolytic viruses, modified vaccinia virus Ankara (MVA), a highly attenuated (VV) is well-studied variant with promising results in preclinical and clinical trials. The Lister VV strain from the Moscow Institute of Viral Preparations (LIVP) has been studied to lesser extent than MVA different property MVA. aim this work was compare efficacy LIVP strains against solid tumors. We developed recombinant variants LIVP-RFP MVA-RFP; enhance onco-selectivity thymidine kinase (TK) gene...
Oncolytic viruses are promising agents for the treatment of various types tumors, including malignant non-Hodgkin lymphomas. In order to identify most effective candidates from a wide panel non-pathogenic antitumor strains, study was carried out their ability reproduce in short-term cultures lymphoid tumor cells. Also, cell were characterized relation presence specific viral receptors CD54, CD55, CD155 on surface.
INTRODUCTION. Developing novel medicines based on non-pathogenic enterovirus strains exhibiting oncotropic and oncolytic properties represents an up-to-date safe approach to complex cancer treatment postoperative metastasis prevention. Safety pharmacology studies are a necessary step in the preclinical development of medicinal products. AIM. The study aimed investigate single repeated-dose general toxicity, local tolerance, safety pharmacology, pyrogenicity products LEV4, LEV7, LEV8, LEV14,...
Основным источником возникновения рецидивов мультиформной глиобластомы после хирургического вмешательства являются стволовые клетки, успевающие проникнуть глубоко в ткани мозга.В настоящее время актуален поиск новых подходов для борьбы с ними, том числе помощью онколитических вирусов.Целью работы было определение чувствительности к непатогенным энтеровирусам клеток человека, поддерживаемых in vitro и модели мышиных ксенотрансплантатов.Культуры опухолевых глиобластом испытывали на...
Transient gene expression is one of the most common methods in molecular biology, equally relevant for basic research projects and biotechnological industries. Despite existence commercial transfection systems, which afford high efficiency levels reporter genes, expanding such systems to industrial scales often problematic due costs reagents. The well-described cationic calcium-phosphate are accessible ensure reproducible results at much lower costs. This study aimed comparative validation...