A5061036657- Gut microbiota and health
- Plant Virus Research Studies
- Diet and metabolism studies
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Plant and Fungal Interactions Research
- Cardiac Health and Mental Health
- Transgenic Plants and Applications
- Radiation Therapy and Dosimetry
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Tryptophan and brain disorders
- Nanoparticles: synthesis and applications
- Probiotics and Fermented Foods
- Vector-Borne Animal Diseases
- Liver Disease Diagnosis and Treatment
- Zoonotic diseases and public health
- Insect Resistance and Genetics
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Cardiovascular Function and Risk Factors
- Clostridium difficile and Clostridium perfringens research
- Infant Nutrition and Health
- Virus-based gene therapy research
Federal Medical-Biological Agency
2017-2025
Center for Strategic Research
2018-2024
Institute of Economic Forecasting
2023
State Research Institute of Highly Pure Biopreparations
2023
Ryazan State Agrotechnological University
2022
Far Eastern Federal University
2021
Doka-Gene (Russia)
2017-2019
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation
2019
MIREA - Russian Technological University
2017-2019
Lomonosov Moscow State University
2009-2018
We report the synthesis and characterization of amorphous iron oxide nanoparticles from salts in aqueous extracts monocotyledonous (Hordeum vulgare) dicotyledonous (Rumex acetosa) plants. The were characterized by TEM, absorbance spectroscopy, SAED, EELS, XPS, DLS methods shown to contain mainly oxohydroxide. H. vulgare produced with diameters up 30 nm. These are intrinsically unstable prone aggregation; however, we rendered them stable long term addition 40 mM citrate buffer pH 3.0. In...
Familial hypercholesterolemia (FH) is a common autosomal codominant disorder, characterized by elevated low-density lipoprotein cholesterol levels causing premature atherosclerotic cardiovascular disease. About 2900 variants of LDLR, APOB, and PCSK9 genes potentially associated with FH have been described earlier. Nevertheless, the genetics in Russian population poorly understood. The aim this study to present data on spectrum gene cohort 595 index patients FH, as well an additional...
Traumatic brain injury (TBI) heavily impacts the body: it damages tissue and peripheral nervous system shifts homeostasis in many types of tissue. An acute compromises “brain–gut-microbiome axis”, a well-balanced network formed by brain, gastrointestinal tract, gut microbiome, which has complex effect: damage to alters composition microbiome; altered microbiome affects TBI severity, neuroplasticity, metabolic pathways through various bacterial metabolites. We modeled rats. Using...
The gut microbiota plays an important role in maintaining human health, as well the development of various pathologies, indicated by a large amount research. One manifestations imbalance microbiome composition is appearance diseases or immune reactions, particular, atopic dermatitis (AD) and/or food allergies (FA). In this research, using 16S NGS sequencing, it was found that children with and can be characterized having higher inflammatory potential. Both groups exhibited abundance...
It is known that the gut microbiota plays an important role in many biological processes of human body. However, all factors and mechanisms influencing changes composition microbiome are not fully understood. As results previous studies show, long-term food preferences play a significant modulating intestinal microbiota. At same time, reflecting peculiarities relationship vitamins, trace elements adequately characterized. Vitamins have bidirectional effect on On one hand, they influence...
Introduction Minimally invasive diagnostics based on liquid biopsy makes it possible early detection of lung cancer (LC). The blood plasma circulating cell-free DNA (cfDNA) fragments reflect the genome and chromatin status are considered as integral biomarkers biological entities for ‘cancer-of-origin’ prediction. aim this work is to create a method processing next-generation sequencing (NGS) data an interpretable binary classification model (CM), which analyzed cfDNA fragmentation features...
BACKGROUND . More than one hundred candidate genes have been associated with longevity, including APOE, FOXO1A, GF1, IGF1, INCR, TP53, etc. Despite extensive research, the precise molecular and genetic underpinnings remain elusive due to low reproducibility of findings. Moreover, most studies focused on exomes neglected intergenic regulatory regions DNA. AIM To examine basis longevity create a prognostic model that assesses chance reaching age 90. MATERIALS AND METHODS study predictors...
BACKGROUND . A trend toward demographic aging of the population is currently observed in most countries worldwide. More and more people are achieving longevity, both due to environmental factors lifestyle, as well molecular genetic processes. Few large-scale studies have examined all contributing longevity Russia globally. Fewer them used a multi-omics approach, which could provide better insight into phenomenon by integrating genomic, epigenomic, transcriptomic metagenomic data. AIM. To...
This research aimed to evaluate the potency of preparation based on heparinized iron oxide nanoparticles (hIONPs) in combination with radiation therapy, including magnetic delivery via applied field (AMF), sarcoma and cervical cancer models. For vitro studies, cells rhabdomyosarcoma (RD), fibrosarcoma (HT1080), (HeLa S3) were treated hIONPs analyzed for survival rate hIONP uptake. Then, cell morphology, cycle, increase reactive oxygen species, mitochondria depolarization, ability form...
Non-alcoholic fatty liver disease (NAFLD) is considered the most common chronic worldwide, affecting nearly 25% of global adult population. Increasing evidence suggests that functional and compositional changes in gut microbiota may contribute to development promote progression NAFLD. 16S rRNA gene next-generation sequencing widely used determine specific features NAFLD microbiome, but a complex system such as requires comprehensive approach. We three different approaches: MALDI-TOF-MS...
Geriatric syndromes (GSs) and aging-associated diseases (AADs) are common side effects of aging. They affecting the lives millions older adults placing immense pressure on healthcare systems economies worldwide. It is imperative to study factors causing these conditions develop a holistic framework for their management. The so-called long-lived individuals-people over age 90 who managed retain much health functionality-could be holding key understanding implications. We analyzed status...
Background . The trend towards aging of the population is global, which makes study etiology, prevention and treatment age-related disorders relevant. Increasing number studies highlights influence 25-hydroxyvitamin D deficiency on health state in older people, as well its correlation with geriatric syndromes prevalence. However, at moment it not known whether these patterns persist patients than 90 due to limited this population. Aim To assess prevalence vitamin a cohort centenarians...
The combination of radiation treatment and chemotherapy is currently the standard for management cancer patients. However, safe doses do not often provide effective therapy, then pre-treated patients are forced to repeat with already increased tumor resistance drugs irradiation. One solutions we suggest improve primary course via enhancing radiosensitivity tumors by magnetic-guided iron oxide nanoparticles (magnetite). We obtained spherical heparinized (hIONPs, ∼20 nm), characterized it TEM,...
Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA similar to viruses of the structurally characterized and well-studied Tobamovirus. Here we report first high-resolution structure BSMV at 4.1 Å obtained by cryo-electron microscopy. We discovered that forms two types virion differ in number coat protein (CP) subunits per turn interactions between CP subunits. While tobacco have fold interact with using conserved residues, axial contacts these viral...
Aim . To assess the concentrations of bacterial and eukaryotic metabolites mainly involved in indole, kynurenine, serotonin pathways tryptophan metabolism a cohort patients with obesity. Materials methods. Using high-performance liquid chromatography mass spectrometric detection, several serum metabolites, such as kynurenic acid, anthranilic xanthurenic quinolinic 5-hydroxyindole-3-acetate, tryptamine, serotonin, indole-3-lactate, indole-3-acetate, indole-3- butyrate,...
Anti-SARS-CoV-2 vaccination leads to the production of neutralizing as well non-neutralizing antibodies. In current study, we investigated temporal dynamics both sides immunity after with two doses Sputnik V against SARS-CoV-2 variants Wuhan-Hu-1 G614-variant (D614G), B.1.617.2 (Delta), and BA.1 (Omicron). First, constructed a pseudovirus assay assess neutralization activity vaccine sera. We show that serum compared D614G is decreased by 8.16-, 11.05-, 11.16- fold in 1, 4, 6 months...
Three 'triple gene block' proteins known as TGBp1, TGBp2 and TGBp3 are required for cell-to-cell movement of plant viruses belonging to a number genera including Hordeivirus. Hordeiviral TGBp1 interacts with viral genomic RNAs form ribonucleoprotein (RNP) complexes competent translocation between cells through plasmodesmata over long distances via the phloem. Binding hordeivirus RNA involves two protein regions, C-terminal NTPase/helicase domain N-terminal extension region. This study...
Conversion of the rod-like tobacco mosaic virus (TMV) virions into "ball-like particles" by thermal denaturation at 90-98 °C had been described R.G. Hart in 1956. We have reported recently that spherical particles (SPs) generated TMV 94-98 were highly stable, RNA-free, and water-insoluble. The SPs uniform shape but varied widely size (53-800 nm), which depended on concentration. Here, we describe some structural characteristics using circular dichroism, fluorescence spectroscopy, Raman...