A5014530329- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Agricultural Productivity and Crop Improvement
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Agriculture and Biological Studies
- Cardiomyopathy and Myosin Studies
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Crop Yield and Soil Fertility
- Genetics and Plant Breeding
- Plant Pathogens and Resistance
- Plant Reproductive Biology
- Agriculture, Plant Science, Crop Management
- Bioenergy crop production and management
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Legume Nitrogen Fixing Symbiosis
- Neurogenetic and Muscular Disorders Research
- Plant pathogens and resistance mechanisms
- Plant Genetic and Mutation Studies
- Garlic and Onion Studies
- Lipoproteins and Cardiovascular Health
- Mitochondrial Function and Pathology
- Cystic Fibrosis Research Advances
National Research Center for Preventive Medicine
2019-2025
All-Russian Scientific Research Institute of Food Biotechnology
2017-2025
Kurchatov Institute
2020-2024
Moscow State Agroengineering University named after V.P. Goryachkin
2011-2024
Ministry of Health of the Russian Federation
2019-2024
Moscow Research Institute" Nemchinovka "
2024
Moscow Institute of Physics and Technology
2024
Skolkovo Foundation
2023
Russian State Agrarian University - Moscow Timiryazev Agricultural Academy
2012-2022
The chromosome preparation is a crucial step for obtaining satisfactory results in molecular cytogenetic researches. of plant chromosomes purposes remains challenge some species. In contrast to human preparation, the processes occurring during and causing spreading are still poorly understood.We studied dynamics after dropping cell suspension on slides. We showed that steam stimulates cytoplasm hydrolysis rapid stretch this spreading. Based these observations, we developed novel method,...
Hemp (Cannabis sativa L.) was karyotyped using by DAPI/C-banding staining to provide chromosome measurements, and fluorescence in situ hybridization with probes for 45 rDNA (pTa71), 5S (pCT4.2), a subtelomeric repeat (CS-1) the Arabidopsis telomere probes. The karyotype has 18 autosomes plus sex pair (XX female XY male plants). are difficult distinguish morphologically, but three pairs could be distinguished Y is larger than autosomes, carries fully heterochromatic DAPI positive arm CS-1...
Familial hypercholesterolemia (FH) is a common autosomal codominant disorder, characterized by elevated low-density lipoprotein cholesterol levels causing premature atherosclerotic cardiovascular disease. About 2900 variants of LDLR, APOB, and PCSK9 genes potentially associated with FH have been described earlier. Nevertheless, the genetics in Russian population poorly understood. The aim this study to present data on spectrum gene cohort 595 index patients FH, as well an additional...
Here, we present a small Russian family, where the index patient received diagnosis of left-ventricular non-compaction cardiomyopathy (LVNC) in combination with skeletal myopathy. Clinical follow-up analysis revealed LVNC phenotype also her son. Therefore, applied broad next-generation sequencing gene panel approach for identification underlying mutation. Interestingly, DES-p.A337P was identified genomes both patients, whereas only carried DSP-p.L1348X. DES encodes muscle-specific...
We performed a targeted sequencing of 242 clinically important genes mostly associated with cardiovascular diseases in representative population sample 1,658 individuals from the Ivanovo region northeast Moscow. Approximately 11% 11,876 detected variants were not found Single Nucleotide Polymorphism Database (dbSNP) or reported earlier Russian population. Most novel singletons and doubletons our sample, virtually no alleles presumably specific for able to reach frequencies above 0.1-0.2%....
Left ventricular noncompaction (LVNC) cardiomyopathy is a disorder that can be complicated by heart failure, arrhythmias, thromboembolism, and sudden cardiac death. The aim of this study to clarify the genetic landscape LVNC in large cohort well-phenotyped Russian patients with LVNC, including 48 families (n=214). All index underwent clinical examination analysis, as well family members who agreed participate and/or testing. testing included next generation sequencing classification...
Digital phenotyping is a fast-growing area of hardware and software research development. Phenotypic studies usually require determining whether there difference in some trait between plants with different genotypes or under conditions. We developed StatFaRmer, user-friendly tool tailored for analyzing time series plant phenotypic parameters, ensuring seamless integration common tasks studies. For maximum versatility across methods platforms, it uses data the form set spreadsheets (XLSX CSV...
Background: Genetic testing for long QT syndrome (LQTS) is straightforward in many families; however, severe and complex cases, a single disease-causing variant may not be enough to explain all clinical features. In such the search genetic modifiers beneficial precise diagnosis management. Case presentation: We describe three-generational family affected with clinically heterogeneous LQTS type 3 bradycardia which novel missense p.V642M HCN4 was identified addition known pathogenic p.E1784K...
Dioecy is relatively rare in plants and sex determination systems vary among such species. A good example of a plant with heteromorphic chromosomes hop <i>(Humulus lupulus)</i>. The genotypes carrying XX or XY correspond to female male plants, respectively. Until now no clear cytogenetic markers for the have been established. Here, first time are clearly identified characterized. high copy sequence (HSR1) has cloned localized on by fluorescence situ hybridization. HSR1 repeat...
Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as source of oil, minerals vitamins. In this study, we analyzed transposable elements satellites its genome. We carried out Illumina DNA sequencing reconstructed main repetitive sequences. For data analysis, developed new bioinformatics approach for advanced satellite analysis showed that about 25% genome consists 24% formed elements, dominated by Ty3/Gypsy...
About 50% of patients with arrhythmogenic cardiomyopathy (ACM) carry a pathogenic or likely mutation in the desmosomal genes. However, there is significant number without positive familial anamnesis. Therefore, molecular reasons for ACM these are frequently unknown and genetic contribution might be underestimated. Here, we used next-generation sequencing (NGS) approach addition single nucleotide polymor-phism (SNP) arrays analysis two independent index medical history. Of note, this strategy...
Transposable element insertions (TEIs) are an important source of genomic innovation by contributing to plant adaptation, speciation, and the production new varieties. The often large, complex genomes make identifying TEIs from short reads difficult expensive. Moreover, rare somatic that reflect mobilome dynamics track using reads. To address these challenges, we combined Cas9-targeted Nanopore sequencing (CANS) with novel pipeline NanoCasTE trace both genetically inherited in plants. We...
The optimization of all constituent conditions to obtain high and even maximum yields is a recent trend in agriculture. Legumes play special role this process, as they have unique characteristics with respect storing protein many other important components their seeds that are useful for human animal nutrition well industry A great advantage legumes the nitrogen fixation activity symbiotic nodule bacteria. This self-sufficiency contributes directly challenging issue feeding world’s growing...
Speciation and allopolyploidization in cereals may be accompanied by dramatic changes abundance of centromeric repeated transposable elements. Here we demonstrate that the reverse transcriptase part Ty3/gypsy retrotransposon (RT-CR) is highly conservative segmental hexaploid Thinopyrum intermedium (JrJvsSt) its possible diploid progenitors Th. bessarabicum (Jb), Pseudoroegneria spicata (St) Dasypyrum villosum (V) but repeats varied to a large extent. Fluorescence situ hybridization (FISH)...
A genetic diagnosis of primary cardiomyopathies can be a long-unmet need in patients with complex phenotypes. We investigated three-generation family cardiomyopathy and various extracardiac abnormalities that had long sought precise diagnosis. The 41-year-old proband hypertrophic (HCM), left ventricular noncompaction, myocardial fibrosis, arrhythmias, short stature. His sister showed HCM, hypertrabeculation sensorineural deafness, congenital genitourinary malformations. Their father...
The germplasm collection of 87 wheat-wheatgrass hybrids developed in Tsitisin Main Botanical Garden (Russia, Moscow) was evaluated for resistance to pre-harvest sprouting (PHS) by spike (SS) and germination index (GI) assays as well grain features. PHS variation haplotype polymorphism the wheatgrass ThVp-1 wheat TaVp-1B genes orthologues Vp-1 revealed studied collection. Four haplotypes were revealed: ThVp-1a (41% entries), ThVp-1b (13%), ThVp-1c (29%), ThVp-1d (15%). association between...
ИСПОЛЬЗОВАНИЕ ГЕНЕТИЧЕСКОГО ПОТЕНЦИАЛА МНОГОЛЕТНИХ ДИКОРАСТУЩИХ ЗЛАКОВ В СЕЛЕКЦИОННОМУЛУЧШЕНИИ ПШЕНИЦЫ (обзор)П.Ю.КРУПИН 1, 2 , М.Г.ДИВАШУК Г.И