A5076965404- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Immune cells in cancer
- Herpesvirus Infections and Treatments
- Characterization and Applications of Magnetic Nanoparticles
- CAR-T cell therapy research
- Sperm and Testicular Function
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Cancer Research and Treatments
- Reproductive System and Pregnancy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Electrochemical Analysis and Applications
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Photodynamic Therapy Research Studies
- Reproductive Biology and Fertility
- Phagocytosis and Immune Regulation
- Viral gastroenteritis research and epidemiology
- Laser-Ablation Synthesis of Nanoparticles
- Iron oxide chemistry and applications
- Immune Cell Function and Interaction
Federal Medical Research Centre for Psychiatry and Narcology
2019-2024
Ministry of Health of the Russian Federation
2011-2024
University of Calgary
2018-2024
Institute of Cancer Research
2024
National University of Science and Technology
2018-2022
Alberta Children's Hospital Research Institute
2016-2022
Alberta Children's Hospital
2016-2022
National University of Science and Technology
2017-2020
Lomonosov Moscow State University
2017-2018
University of Science and Technology
2018
High-quality, 25 nm octahedral-shaped Fe
Radiographic contrast agents cause acute kidney injury (AKI), yet the underlying pathogenesis is poorly understood. Nod-like receptor pyrin containing 3–deficient (Nlrp3-deficient) mice displayed reduced epithelial cell and inflammation in a model of contrast-induced AKI (CI-AKI). Unexpectedly, directly induced tubular death vitro that was not dependent on Nlrp3. Rather, activated canonical Nlrp3 inflammasome macrophages. Intravital microscopy revealed diatrizoate (DTA) uptake within minutes...
Despite significant advances in our knowledge regarding the genetics and molecular biology of gliomas over past two decades hundreds clinical trials, no effective therapeutic approach has been identified for adult patients with newly diagnosed glioblastoma, overall survival remains dismal. Great hopes are now placed on combination immunotherapy. In immunotherapeutics generally tested after standard therapy (radiation, temozolomide, steroid dexamethasone) or concurrently temozolomide and/or...
Second mitochondrial activator of caspase (Smac)-mimetic compounds and oncolytic viruses were developed to kill cancer cells directly. However, Smac-mimetic compound virus therapies also modulate host immune responses in ways we hypothesized would complement one another promoting anticancer T-cell immunity. We show that synergize driving CD8
Liposomes are the most extensively used nanocarriers in cancer therapy. Despite advantages these vehicles provide over free drugs, there still limitations with regards to efficiency of liposomes delivery tumors and off-target accumulation. A better understanding nanodrugs extravasation mechanisms different tumor types normal vessels is needed improve their antitumor activity. We intravital microscopy track for fluorescent behavior xenograft models (murine breast 4T1 melanoma B16, human...
The influenza A virus (IAV) causes a respiratory tract infection with approximately 10% of the population infected by each year. Severe IAV is characterized excessive inflammation and tissue pathology in lungs. Platelet neutrophil recruitment to lung are involved pathogenesis IAV, but specific mechanisms have not been clarified. Using confocal intravital microscopy mouse model infection, we observed profound recruitment, platelet aggregation, extracellular trap (NET) production thrombin...
Magnetic nanoparticles (MNPs) are widely considered for cancer treatment, in particular magnetic hyperthermia (MHT). Thereby, MNPs still being optimized lowest possible toxicity on organisms while the properties matched best heating capabilities. In this study, biocompatibility of 12 nm cobalt ferrite MNPs, functionalized with citrate ions, different dosages mice and rats both sexes was investigated 30 days after intraperitoneal injection. The animals’ weight, behavior, blood cells changes,...
Conventional dendritic cells (cDCs) generate protective cytotoxic T lymphocyte (CTL) responses against extracellular pathogens and tumors. This is achieved through a process known as cross-presentation (XP), and, despite its biological importance, the mechanism(s) driving XP remains unclear. Here, we show that cDC-specific pore-forming protein called apolipoprotein L 7C (APOL7C) up-regulated in response to innate immune stimuli recruited phagosomes. Association of APOL7C with phagosomes led...
CAR T therapy for solid tumors is limited by a lack of safe and uniformly expressed cell-surface targets. Here, we identify the MiT fusion-driven protein GPNMB as being highly, homogeneously, stably in primary relapsed translocation-positive alveolar soft part sarcoma (ASPS) renal cell carcinoma (tRCC). We developed GPNMB-targeting called GCAR1 that shows activity against patient-matched cells, organoids xenograft models. First-in-human treatment patient with metastatic ASPS was well...
Summary Acute and chronic infections of the seminal tract are among most common causes male infertility. As at least half infertility cases classified as idiopathic, some these might be attributed to asymptomatic infection. The detection quantification Epstein–Barr virus ( EBV ), cytomegalovirus CMV ) human herpes type 6 HHV ‐6) DNA in semen samples were performed. A total 232 patients divided into five groups: (i) infertile men with varicocoele; (ii) idiopathic infertility; (iii)...
Oncolytic viral therapy is a promising novel approach to cancer treatment. viruses cause tumor regression through direct cytolysis on the one hand and recruiting activating immune cells other. In this study, enhance antitumor efficacy of thymidine kinase-deficient vaccinia virus (VV, Lister strain), recombinant variants encoding bacterial flagellin (subunit B) Vibrio vulnificus (LIVP-FlaB-RFP), firefly luciferase (LIVP-Fluc-RFP) or red fluorescent protein (LIVP-RFP) were developed. The...
Insufficient drug accumulation in tumors is still a major concern for using cancer nanotherapeutics. Here, the neutrophil-based delivery of three nanoparticle types—liposomes, PLGA, and magnetite nanoparticles—was assessed both vitro vivo. Confocal microscopy flow cytometry analysis demonstrated that all studied nanoparticles interacted with neutrophils from peripheral blood mice 4T1 mammary adenocarcinoma without significant impact on neutrophil viability or activation state. Intravital...
Background/Objectives: Neutrophils are emerging as promising candidates for cell-based nanodrug delivery to tumors due their unique biological properties. This study aims investigate the mechanisms of nanoparticle internalization by neutrophils, specifically focusing on liposomes, poly(lactic-co-glycolic acid) (PLGA), and magnetite nanoparticles. Understanding these could enhance efficiency neutrophil-based cancer treatment. Methods: were isolated from peripheral blood mice bearing 4T1...
The presence of human cytomegalovirus (HCMV) in male genital tract suggests its vertical transmission with spermatozoa and the development a potentially dangerous fetal infection. objective present study was to evaluate possibility intracellular HCMV localization germ cells examine effect virus on spermatogenesis. Semen samples from 91 infertile 47 fertile men were analyzed. detected by real time PCR, rapid culture method PCR situ. Human testis organotypic quantitative karyological analysis...
Herein, we describe the design, synthesis, and biological evaluation of novel betulin N-acetyl-d-galactosamine (GalNAc) glycoconjugates suggest them as targeted agents against hepatocellular carcinoma. We prepared six conjugates derived via C-3 C-28 positions with one or two saccharide ligands. These molecules demonstrate high affinity to asialoglycoprotein receptor (ASGPR) hepatocytes assessed by in silico modeling surface plasmon resonance tests. Cytotoxicity studies vitro revealed a...