A5087369314- Glioma Diagnosis and Treatment
- Nanoparticle-Based Drug Delivery
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Nanoparticles: synthesis and applications
- RNA Interference and Gene Delivery
- Cancer Immunotherapy and Biomarkers
- Viral Infectious Diseases and Gene Expression in Insects
- MicroRNA in disease regulation
- Autism Spectrum Disorder Research
- Adenosine and Purinergic Signaling
- Advanced Proteomics Techniques and Applications
- Diabetes and associated disorders
- Cancer Research and Treatments
- Laser-Ablation Synthesis of Nanoparticles
- Neurological Disease Mechanisms and Treatments
- CAR-T cell therapy research
- Animal Genetics and Reproduction
- Brain Metastases and Treatment
- Photodynamic Therapy Research Studies
- Gender Roles and Identity Studies
- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
Federal Medical Research Centre for Psychiatry and Narcology
2020-2024
Ministry of Health of the Russian Federation
2021-2024
Despite significant advances in our knowledge regarding the genetics and molecular biology of gliomas over past two decades hundreds clinical trials, no effective therapeutic approach has been identified for adult patients with newly diagnosed glioblastoma, overall survival remains dismal. Great hopes are now placed on combination immunotherapy. In immunotherapeutics generally tested after standard therapy (radiation, temozolomide, steroid dexamethasone) or concurrently temozolomide and/or...
The effectiveness of tumor therapy, especially immunotherapy and oncolytic virotherapy, critically depends on the activity host immune cells. However, various local systemic mechanisms immunosuppression operate in cancer patients. Tumor-associated involves deregulation many components immunity, including a decrease number T lymphocytes (lymphopenia), an increase levels or ratios circulating tumor-infiltrating immunosuppressive subsets [e.g., macrophages, microglia, myeloid-derived suppressor...
Background/Objectives: Neutrophils are emerging as promising candidates for cell-based nanodrug delivery to tumors due their unique biological properties. This study aims investigate the mechanisms of nanoparticle internalization by neutrophils, specifically focusing on liposomes, poly(lactic-co-glycolic acid) (PLGA), and magnetite nanoparticles. Understanding these could enhance efficiency neutrophil-based cancer treatment. Methods: were isolated from peripheral blood mice bearing 4T1...
Insufficient drug accumulation in tumors is still a major concern for using cancer nanotherapeutics. Here, the neutrophil-based delivery of three nanoparticle types—liposomes, PLGA, and magnetite nanoparticles—was assessed both vitro vivo. Confocal microscopy flow cytometry analysis demonstrated that all studied nanoparticles interacted with neutrophils from peripheral blood mice 4T1 mammary adenocarcinoma without significant impact on neutrophil viability or activation state. Intravital...
Ad5-delta-24-RGD is currently the most clinically advanced recombinant adenovirus (rAd) for glioma therapy. We constructed a panel of fiber-modified rAds (Ad5RGD, Ad5/3, Ad5/35, Ad5/3RGD, and Ad5/35RGD, all harboring delta-24 modification) compared their infectivity, replication, reproduction, cytolytic efficacy in human rodent cell lines short-term cultures from primary gliomas. In cells, both Ad5/35-delta-24 Ad5/3-delta-24 displayed superior infectivity over Ad5-delta-24-RGD, while...
Ischemic stroke triggers a whole cascade of pathological changes in the brain, one which is postischemic inflammation. Since such cases thrombolytic therapy often not possible, methods that modulate inflammation and affect microglia become particularly interesting. We synthesized 3-(2-oxo-4-phenylpyrrolidin-1-yl)propane-1-sulfonate calcium(II) (Compound 4) studied its anti-inflammatory activity vitro vivo models ischemia. Macrophage cell line RAW 264.7 was treated with lipopolysaccharides...
As a result of comprehensive approach to comparative assessment the activity genetically modified adenoviruses on panel tumor cell cultures, we obtained unique viral preparation with combination modifications in i-leader and E3-19K genes, which has enhanced oncolytic properties when compared its genetic predecessors.
Цель. Исследовать возможное влияние локуса IDDM18 на предрасположенность к СД1 в семьях из русской популяции. Материалы и методы. Исследованы ДНК двух групп семей городских популяций Москвы Самары, с конкордантными (27 семей) дискордантными (62 семьи) парами сибсов. Выделяли геномную крови больных, определяли генотипы полиморфных маркеров. Амплифицированные фрагменты ДНК, содержащие полиморфные маркеры, разделяли 10% полиакриламидном геле последующей окраской азотнокислым серебром. Фрагменты...
We perfomed panoramic proteomic analysis of rat C6 glioma cells, native astrocytes and co- cultivated with cell line. Based on the data, a putative model was present for diagnostic differentiation normal from cells.
Human multiforme glioblastoma is characterized by an unfavorable prognosis, low survival rate and extremely limited possibilities for therapy. Rat C6 glioma experimental model the study of growth invasion. It has been shown that development tumor accompanied changes in surrounding normotypic tissues [1]. These create a favorable environment give it evolutionary advantage [2]. Description occurring cells body upon their contact with great interest. We have grown rat astrocytes, as well...