Benzyl isothiocyanate (BITC), a component of dietary cruciferous vegetables, has antioxidant and anticancer properties. In this study, we show for the first time antimetastatic effects BITC in human colon cancer HT29 cells. had an inhibitory effect on cell migration invasion. Protein levels matrix metalloproteinase-2 (MMP-2), metalloproteinase-9 (MMP-9), urokinase-plasminogen activator (u-PA) were reduced by concentration-dependent manner. also exerted phosphorylation c-Jun N-terminal kinase...

Berberine (BBR) is a natural alkaloid with significant antitumor activities against many types of cancer cells. In this study, we investigated the molecular mechanisms by which BBR repressed metastatic potential breast was found to downregulate enzymatic and expression levels matrix metalloproteinases 2 9 (MMP2 MMP9, respectively). The BBR-mediated suppression MMP2 MMP9 involved inhibition Akt/nuclear factor kappa B (NF-κB) activator protein-1 (AP-1) signaling pathways. Furthermore, Akt...

Green tea is a widely consumed beverage known for its beneficial anti-inflammatory, anti-oxidative, anti-mutagenic, anti-carcinogenic, and cardioprotective properties. Here, we administered epigallocatechin gallate fraction of green extract (EGTE) to mice 6 weeks examined the effects on innate adaptive immune responses by measuring phagocytic natural killer (NK) cell activity, as well antigen-specific proliferation, cytolysis, cytokine secretion, antibody production. Our data show that EGTE...

ABSTRACT Triptolide, a traditional Chinese medicine, obtained from Tripterygium wilfordii Hook F, has anti‐inflammatory, antiproliferative, and proapoptotic properties. We investigated the potential efficacy of triptolide on murine leukemia by measuring triptolide‐induced cytotoxicity in WEHI‐3 cells vitro . Results indicated that induced cell morphological changes cytotoxic effects through G0/G1 phase arrest, induction apoptosis. Flow cytometric assays showed increased production reactive...

Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed develop in vitro plant regeneration system of P. via somatic embryogenesis analyse chlorogenic acid rutin contents a few commercially available products Japan Taiwan markets, tissue culture plants derived from embryos. Induction could be achieved...

Abstract Oral cancer is one of the cancer‐related diseases in human populations and its incidence rates are rising worldwide. Fisetin, a flavonoid from natural products, has been shown to exhibit anticancer activities many cell lines but molecular mechanism fisetin‐induced apoptosis oral cells still unclear; thus, this study, we investigated death associated signal pathways on SCC‐4 vitro . We examined morphological changes, total viable cells, cycle distribution by phase contrast microscopy...

Bufalin has been shown to exhibit multiple pharmacological activities, including induction of apoptosis in many types cancer cell lines. Osteosarcoma is a type which difficult treat and the purpose this study was investigate effects bufalin on migration invasion human osteosarcoma U‐2 OS cells. The wound healing assay Boyden chamber transwell were used for examining Western blotting gelatin zymography assays theexpression activities metalloproteinase (MMP)‐2, MMP‐7 or MMP‐9 levels. analysis...

Abstract Novel 2‐phenyl‐4‐quinolone compounds have potent cytotoxic effects on different human cancer cell lines. In this study, we examined anticancer activity and mechanisms of 20‐fluoro‐6,7‐methylenedioxy‐2‐phenyl‐4‐quinolone (CHM‐1) in osterogenic sarcoma U‐2 OS cells. CHM‐1‐induced apoptosis was determined by flow cytometric analysis, DAPI staining, Comet assay, caspase inhibitors. CHM‐1‐inhibited migration invasion assessed a wound healing gelatin zymography, Transwell assay. The CHM‐1...

Abstract Numerous evidences have shown that chrysin induced cytotoxic effects via cell cycle arrest and induction of apoptosis in human cancer lines, however, no information showed inhibited skin migration invasion. In this study, we investigated anti‐metastasis mechanisms melanoma A375.S2 cells vitro. Under sub‐lethal concentrations (0, 5, 10, 15 μM) which inhibits mobility, invasion were assayed by wound healing Transwell filter. That MMP‐2 activity was gelatin zymography assay. Western...

Lung cancer notably contributes to tumor-associated mortality worldwide, and standard chemotherapy is used for lung patients. However, its therapeutic efficacy remains unsatisfactory. This study aimed evaluate the effects molecular mechanisms of sorafenib bufalin combination therapy on cells in vitro.NCI-H292 were treated with sorafenib, bufalin, bufalin. Cell viability, ROS production, Ca2+ release, mitochondrial membrane potential examined by flow cytometric assay. Annexin V/PI staining...

This study was performed to investigate the free radical scavenging active components from in vitro propagated medicinal herbs of genus Dendrobium, namely, Dendrobium tosaense Makino and moniliforme SW, using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidative assay. Seeds capsules derived after 12 weeks hand-pollination germinated asymbiotically (50−74%) on half-strength Murashige Skoog's (MS) basal medium with 3% sucrose solidified 0.9% Difco agar. Active growth seedlings achieved by...

Saussurea involucrata (Kar. et Kir.) commonly known as 'snow lotus' or 'Xue Lian' is an important plant in the traditional Chinese system of medicine. The contains flavonoids such syringin and rutin. These compounds have been reported to be anti-rheumatic, anti-inflammatory dilate blood vessels, lower pressure, prevent cardiovascular diseases, enhance immunity, act anti-aging, anti-cancer, anti-fatigue agents. species has become endangered due excessive collection S. plants wild, slower...

Abstract Prostate cancer is the most common male reproductive system cancer. The prevalence of prostate in Europe and United States higher than that Asian region. However, treatment remains unsatisfactory. Psoralea corylifolia has been used to cure this disease as Chinese medicine In study, we analyzed components ethanol extraction unprepared prepared P. by HPLC. Psoralen isopsoralen content from twofold unprepared, so use study. effects (PCE) on PC‐3 human cells remain unclear. were treated...

Abstract Tetrandrine (TET) has been reported to induce anti‐cancer activity in many human cancer cells and also inhibit cell migration invasion. However, there are no reports show TET inhibits invasion brain glioblastoma multiforme GBM 8401 cells. In this study, we investigated the anti‐metastasis effects of on vitro. Under sub‐lethal concentrations (from 1, 5 up 10 μM), significantly inhibited mobility, that were assayed by wound healing Transwell assays. Gelatin zymography assay showed...

A simple and efficient plant propagation system has been developed by asymbiotic germination of seeds in three medicinally important Dendrobium species, namely, tosaense, moniliforme, linawianum. Plants obtained from natural habitats were grown the greenhouse. The flowers hand pollinated. Seeds capsules derived after 12 weeks hand-pollination germinated asymbiotically (50—74%) on half strength Murashige Skoog's (MS) basal medium with 3% sucrose solidified 0.9% Difco agar. Active growth...

Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical chemical compositions which might affect the biological activities. Although many chromatographic spectrometric methods reported determine concentration betaine other bioactive amino acids, disturbance resulted polar substances with low UV-absorbance expensive mass facilities reduced applicability...