A5049298261- Prostate Cancer Treatment and Research
- MicroRNA in disease regulation
- Fungal Biology and Applications
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- Genetic Associations and Epidemiology
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Phytochemistry and biological activity of medicinal plants
- Circular RNAs in diseases
- Phytochemistry and Bioactivity Studies
- Ginseng Biological Effects and Applications
- Mycorrhizal Fungi and Plant Interactions
- Nitric Oxide and Endothelin Effects
- Endoplasmic Reticulum Stress and Disease
- Bioactive Compounds and Antitumor Agents
- Chemical synthesis and alkaloids
- Traditional and Medicinal Uses of Annonaceae
- Barrier Structure and Function Studies
- Receptor Mechanisms and Signaling
- Alkaloids: synthesis and pharmacology
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- Cell Adhesion Molecules Research
- Sexual Differentiation and Disorders
China Medical University
2013-2023
China Medical University
2009-2012
Kaohsiung Medical University
2009-2010
Kaohsiung Municipal Hsiao-Kang Hospital
2009-2010
National Taiwan University Hospital
2010
China Medical University Hospital
2009-2010
Kaohsiung Medical University Chung-Ho Memorial Hospital
2009-2010
Tri-Service General Hospital
2010
Oriental Institute of Technology
2009
This study demonstrated aloe‐emodin‐ and emodin‐induced apoptosis in lung carcinoma cell lines CH27 (human squamous cell) H460 non‐small cell). Aloe‐emodin‐ was characterized by nuclear morphological changes DNA fragmentation. During apoptosis, an increase cytochrome c of cytosolic fraction activation caspase‐3, identified the cleavage its proform, were observed. To elucidate whether expression protein kinase C (PKC) isozymes are involved this examined PKC Western blotting techniques during...
Emodin (1,3,8‐trihydroxy‐6‐methylanthraquinone) is an active component from the root and rhizome of Rheum palmatum that has been reported to exhibit antitumour effects, but mechanism not known. The study investigated effects mechanisms emodin‐induced cell death in human lung squamous carcinoma line CH27. (50 μ M )‐induced CH27 apoptosis was confirmed by morphological change, sub‐G 1 formation flow cytometry analysis, viability assay degradation focal adhesion kinase this study....
Abstract Recent genomewide association studies have identified several prostate cancer susceptibility variants. However, the between these variants and biochemical failure in patients receiving radical prostatectomy has not been determined. We systematically evaluated 20 cancer–associated single-nucleotide polymorphisms a cohort of 320 localized prostatectomy. Each polymorphism found to be associated with recurrence prostate-specific antigen was further analyzed by Kaplan-Meier analysis Cox...
Abstract Purpose: Recent evidence indicates that small noncoding RNA molecules, known as microRNAs (miRNAs), are involved in cancer initiation and progression. We hypothesized genetic variations miRNAs miRNA target sites could be associated with the efficacy of androgen-deprivation therapy (ADT) men prostate cancer. Experimental Design: systematically evaluated 61 common single nucleotide polymorphisms (SNPs) inside a cohort 601 advanced treated ADT. The prognostic significance these SNPs on...
Ganoderma lucidum is one of most widely used herbal medicine and functional food in Asia, ganoderic acids (GAs) are its active ingredients. Regulation GA biosynthesis enhancing production critical to using G. as a medicine. However, regulation by various signaling remains poorly understood. This study investigated the role apoptosis on presented novel approach, namely induction, increasing production. Aspirin was able induce cell lucidum, which identified terminal deoxynucleotidyl...
Recent evidence indicates that microRNAs might participate in prostate cancer initiation, progression and treatment response. Germline variations alter target gene expression modify the efficacy of therapy. To determine whether genetic variants microRNA sites are associated with risk biochemical recurrence (BCR) after radical prostatectomy (RP). We retrospectively studied two independent cohorts composed 320 Asian 526 Caucasian men pathologically organ-confined who had a median follow-up...
Accumulating evidence indicates that oestrogens have significant direct effects on normal prostate development and carcinogenesis. The majority of the biological activities are mediated through oestrogen receptor (ER), which functions as a hormone-inducible transcription factor to regulate target gene expression by binding response elements (EREs) in regulatory regions genes. Sequence variants EREs might affect ER-ERE interaction subsequent physiological activities. Therefore, we tested...
Apoptosis is an essential physiological process that controls many important biological functions. However, apoptosis signaling in relation to secondary metabolite biosynthesis plants and fungi remains a mystery. The fungus Ganoderma lucidum popular herbal medicine worldwide, but the biosynthetic regulation of its active ingredients (ganoderic acids, GAs) poorly understood. We investigated role 3',5'-cyclic adenosine monophosphate (cAMP) fungal GA G. lucidum. Two phosphodiesterase inhibitors...
Abstract Aloe‐emodin (1,8‐dihydroxy‐3‐(hydroxymethyl)‐anthraquinone) is an active component from the root and rhizome of Rheum palmatum that has been reported to exhibit antitumor effects through unknown mechanism. Our study investigated mechanisms aloe‐emodin‐induced cell death in human lung nonsmall carcinoma line H460. (40 μM)‐induced apoptosis H460 cells involves modulation cAMP‐dependent protein kinase, kinase C, Bcl‐2, caspase‐3 p38 expression. The relationship various signals involved...
Abstract This study demonstrated that fenofibrate, a lipid-lowering drug, induced significant time-dependent cytotoxicity of hepatoma Hep3B cells. cells are significantly more sensitive to cell killing by fenofibrate than HepG2, lung cancer CH27 and oral HSC-3 From the result docking simulation, can bind excellently thioesterase domain fatty acid synthase (FASN) binding site as orlistat, FASN inhibitor, acts. The fenofibrate-induced was protected addition palmitate, indicating cytotoxic...
Orlistat has been proved to be an effective fatty acid synthase inhibitor that is able inhibit the proliferation and induce apoptosis in many cancer cell types. However, anticancer effects of orlistat on hepatocellular carcinoma are undefined. We found inhibited growth induced G0/G1 cycle arrest with increased cyclin D, E, p21 expression human hepatoma Hep3B cells. Furthermore, protein A, B, Cdk1, Cdk2, Cdk4 was reduced by orlistat. This study investigated role lipid metabolism...
What’s known on the subject? and What does study add? Although most clinically localized prostate cancer patients who underwent radical prostatectomy have a favorable outcome, molecular markers capable of providing prognostic information are still urgently needed to identify high‐risk might benefit from aggressive treatment. In this study, we found that RUNX1 rs2253319 polymorphism was significantly associated with higher risks advanced pathological stage, lymph node metastasis, time disease...
Submerged cultures of Ganoderma lucidum are used to produce fungal mycelium, which is as a functional food and in the production various triterpenoids, including ganoderic acids (GAs). Specific culture approaches that mycelium with high levels GAs good biological activity critical industry. In this study, solid-medium approach producing was compared submerged system. Production GAs, biomass, intracellular polysaccharides, cytotoxicity cultured were between solid culture. Growing G. strains...
Two new norsesquiterpenoids, solanerianones A and B (1–2), together with nine known compounds, including four sesquiterpenoids, (−)-solavetivone (3), (+)-anhydro-β-rotunol (4), solafuranone (5), lycifuranone (6); one alkaloid, N-trans-feruloyltyramine (7); fatty acid, palmitic acid (8); phenylalkanoid, acetovanillone (9), two steroids, β-sitosterol (10) stigmasterol (11) were isolated from the n-hexane-soluble part of roots Solanum erianthum. Their structures elucidated on basis physical...
Abstract Ultraconserved regions (UCRs) are DNA segments of longer than 200 bp in length that completely conserved between human, rat, and mouse genomes. Recent studies have shown UCRs frequently located at fragile sites involved cancers, their levels transcription can be altered during human tumorigenesis. We systematically evaluated 14 common single-nucleotide polymorphisms (SNPs) within three cohorts prostate cancer patients, to test the hypothesis these UCR SNPs might influence clinical...
Aloe-emodin (1,8-dihydroxy-3-(hydroxymethyl)-anthraquinone) is one of the active constituents from root and rhizome Rheum palmatum. Our previous study has demonstrated that aloe-emodin induced a significant change in expression lung cancer cell apoptosis-related proteins compared to those control cells. However, molecular mechanisms underlying biological effects still remain unknown. Based on these reasons, we were interested aloe-emodin–induced total protein by proteomics technique during...
Abstract Androgen‐deprivation therapy (ADT) is the most common for advanced prostate cancer, but prognosis significantly differs among individuals. In this study, we evaluated recently identified 19 cancer susceptibility variants as prognostic predictors survival after ADT. A total of 601 patients treated with ADT were enrolled in study cohort. The significance risk on disease progression, cancer‐specific mortality (PCSM) and all‐cause (ACM) assessed by Kaplan–Meier analysis Cox regression...