A5022010854- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Retinal and Macular Surgery
- Glaucoma and retinal disorders
- Ocular Diseases and Behçet’s Syndrome
- Retinal and Optic Conditions
- Retinal Imaging and Analysis
- Intraocular Surgery and Lenses
- Ocular Infections and Treatments
- Ocular Disorders and Treatments
- Vascular Malformations Diagnosis and Treatment
- Ophthalmology and Visual Impairment Studies
- Corneal surgery and disorders
- melanin and skin pigmentation
- Traumatic Ocular and Foreign Body Injuries
- RNA regulation and disease
- interferon and immune responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Ocular Oncology and Treatments
- Neuroscience and Neuropharmacology Research
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- Systemic Lupus Erythematosus Research
- Photoreceptor and optogenetics research
- MicroRNA in disease regulation
Eye & ENT Hospital of Fudan University
2016-2025
Fudan University
2014-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2025
Ministry of Health
2023
Academy of Medical Sciences
2023
Academia Sinica
2023
Ministry of Health
2023
Peking Union Medical College Hospital
2023
Science and Technology Commission of Shanghai Municipality
2017-2022
National Health and Family Planning Commission
2019-2022
Nuclear movement relative to cell bodies is a fundamental process during certain aspects of mammalian retinal development.During the generation photoreceptor cells in division cycle, nuclei progenitors oscillate between apical and basal surfaces neuroblastic layer (NBL).This termed interkinetic nuclear migration (INM).Furthermore, newly formed migrate form outer (ONL).In current study, we demonstrated that KASH domain-containing protein, Syne-2/Nesprin-2, as well SUN proteins, SUN1 SUN2,...
Diabetic retinopathy, a vascular complication of diabetes mellitus, is the leading cause visual impairment and blindness. circRNAs act as competing endogenous RNA, sponging target miRNA thus influencing mRNA expression in diseases. We investigated whether how circDNMT3B involved retinal dysfunction under diabetic conditions.qRT-PCR was performed to detect circDNMT3B, miR-20b-5p, BAMBI microvascular endothelial cells conditions. Western blot, Cell Counting Kit-8, Transwell, Matrigel tube...
Rationale: The Notch and transforming growth factor-β (TGFβ) signaling pathways are two intracellular mechanisms that control fibrosis in general but whether they play a major role retinal is less clear. Here we study how these regulate Müller cell-dominated vitro vivo. Methods: Human MIO-M1 cells were treated with ligands TGFβ1, either alone or combination. Western blots performed to changes γ-secretase proteases, downstream effectors, endogenous phosphorylated Smad3 (p-Smad3) extracellular...
Purpose: The purpose of this study was to evaluate the predisposing risk factors and treatment outcomes endogenous fungal endophthalmitis (EFE). Methods: Records 25 patients (29 eyes), diagnosed with EFE treated at Shanghai Eye, Ear, Nose & Throat Hospital from January 2002 December 2006, were retrospectively reviewed. Four had bilateral disease. Final visual acuity recurrence evaluated. Results: Of these EFE, 20 (22 eyes) by pars plana vitrectomy. remaining 5 (7 3 cases (4 cured antifungal...
Purpose: To investigate the visual outcomes and occurrences of perioperative complications after three-dimensional (3D) heads-up vitrectomy compared with traditional microscopic (TM) surgery for vitreoretinal diseases. Methods: A retrospective case series comparison 3D TM performed on 124 202 eyes a minimum follow-up 6 months, respectively, by same surgeon. Changes in acuity, primary anatomical success, operation time, were evaluated. Results: There no significant differences age, gender,...
Aims To investigate the frequency of USH2A mutation and clinical genetic differences between Usher syndrome type II (USH2) retinitis pigmentosa (RP) in a large cohort Chinese patients. Methods A total 1381 patients with inherited retinal disease (IRD) were recruited. The phenotypic genotypic information mutations was evaluated. Results prevalence 15.75%, which most frequently detected gene this Hotspot c.8559-2A >G c.2802T >G. Patients USH2 had an earlier more serious decline visual...
Purpose: Pathologic myopia (PM) is one of the primary causes blindness. This study aims to explore possible relations between composition microRNA in vitreous exosomes patients with PM and progression myopic maculopathy. Methods: Vitreous humor (VH) samples were collected from undergoing retinal surgery. A total 15 12 VH obtained control, respectively. The group was divided into PM-L (G2) PM-H groups (G3 G4) order differentially expressed microRNAs (DEMs) that account for relatively poor...
Abstract Background To investigate the anatomical and functional outcomes of macular hole-associated retinal detachment (MHRD) after vitrectomy using inverted internal limiting membrane (ILM) flap technique with autologous blood clot (ABC). Methods This retrospective observational study included 80 eyes MHRD that underwent ILM without (46 eyes) or ABC (34 eyes). Fundus photography optical coherence tomography were evaluated. The pre- postoperative best-corrected visual acuities (BCVAs) BCVA...
Purpose To observe the changes in axial length (AL) patients of acute retinal necrosis (ARN) undergoing vitrectomy and investigate correlated factors.
To investigate the optical coherence tomography angiography (OCTA) characteristics of diabetic macular edema (DME) at different stages.This study was a cross-sectional study. Patients diagnosed with DME were recruited. classified into early, advanced, and severe DME. The vessel density (VD) in superficial vascular plexus (SVP), deep (DVP) foveal avascular zone (FAZ) parameters, including FAZ area, perimeter, acircularity index VD 300-μm-wide region around (FD-300), calculated by AngioVue...
Purpose: To determine the effect of three-month diabetes on retinal ganglion cell (RGC) morphology and density. Methods: Experimental was induced in Sprague-Dawley rats by intraperitoneal injection streptozotocin (STZ). Retinas from diabetic age-matched control were harvested, immunohistochemistry with monoclonal anti-Thy-1 antibody carried out for calculating RGC Random labeling gene gun propelled lipophilic fluorescent dye, DiI, coated particles (DiOlistic method) done detailed...
Abstract Background We aim to study the level of inflammatory cytokines in aqueous post‐vitrectomy eyes. Methods Aqueous samples were obtained from eyes that underwent cataract surgery. Samples age‐related patients also collected as controls. The concentrations measured by array‐based multiplex sandwich enzyme‐linked immunosorbent assay system and microparticle‐based cytokine assay. Results Eighteen 18 included; all received one vitrectomy with gas injection for rhegmatogenous retinal...
Purpose: To evaluate vitreous humor (VH) protein expression profiles in patients with proliferative diabetic retinopathy (PDR), and without intravitreal injection (IVI) of anti-vascular endothelial growth factor (anti-VEGF) before vitrectomy. Methods: We enrolled consecutive PDR who needed pars plana vitrectomy (PPV) or IVI pan-retinal photocoagulation (PRP). Visual acuity, duration, treatment diabetes mellitus, ocular history, fundus examinations were recorded. VH samples collected...
To clarify the correlations between changes in photoreceptor layer (PRL) and other clinical characteristics during central serous chorioretinopathy.Patients with chorioretinopathy one eye affected were enrolled. Photoreceptor appearance within detached area was evaluated, its symptom duration, best-corrected visual acuity, difference foveal outer nuclear thickness contralateral eyes analyzed.A total of 222 patients included. The PRL border appeared either smooth, granulated, or as scattered...
Background Bestrophin-1 ( BEST1 ) gene is associated with a wide range of ocular phenotypes, collectively termed as bestrophinopathy. The aim the current study was to identify mutation spectrum in large cohort Chinese patients Methods Patients clinically suspected bestrophinopathy were screened using multigene panel testing. All variants confirmed by Sanger sequencing, and validated families. Findings A total 92 (Best vitelliform macular dystrophy (BVMD)=77; autosomal recessive (ARB)=15)...
Purpose: To clarify the mutation spectrum and frequency of ABCA4 in a Chinese cohort with Stargardt disease (STGD1). Methods: A total 153 subjects, comprising 25 families (25 probands their family members) 71 sporadic cases, were recruited for analysis variants. All STGD1 underwent comprehensive ophthalmologic examination. Overall, 792 genes involved common inherited eye diseases screened variants by panel-based next-generation sequencing (NGS). Variants filtered analyzed to evaluate...
Purpose: To describe the multimodal imaging characteristics of flat irregular pigment epithelial detachment (FIPED) in patients with chronic central serous chorioretinopathy and determine risk factors for vascularized FIPED to explore activity before after half-dose photodynamic therapy. Methods: Multimodal data 185 eyes 155 consecutive included spectral domain optical coherence tomography, fluorescein angiography, indocyanine green angiography. Optical tomography angiography was available...