A5085631728- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Digital Holography and Microscopy
- Optical Imaging and Spectroscopy Techniques
- Cell Image Analysis Techniques
- Plasmonic and Surface Plasmon Research
- Metabolomics and Mass Spectrometry Studies
- Nanoplatforms for cancer theranostics
- Molecular Biology Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Laser-induced spectroscopy and plasma
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Infrared Thermography in Medicine
- Identification and Quantification in Food
- Electrochemical sensors and biosensors
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Ear Surgery and Otitis Media
- Microfluidic and Bio-sensing Technologies
- Advanced Chemical Sensor Technologies
- Mass Spectrometry Techniques and Applications
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2016-2025
Sidney Kimmel Comprehensive Cancer Center
2016-2024
Uttaranchal University
2024
Chandigarh University
2024
Vellore Institute of Technology University
2024
Assam Down Town University
2024
University of Baltimore
2015-2023
Tumori Foundation
2022
Second Hospital of Shandong University
2017
Biogenic synthesis of metal nanoparticles is considerable interest, as it affords clean, biocompatible, nontoxic, and cost-effective fabrication. Driven by their ability to withstand variable extremes environmental conditions, several microorganisms, notably bacteria fungi, have been investigated in the never-ending search for optimal nanomaterial production platforms. Here, we present a hitherto unexplored algal platform featuring Chlorella pyrenoidosa, which offers high degree consistency...
Driven by the need to engineer robust surface coatings for medical devices prevent infection and sepsis, incorporation of nanoparticles has surfaced as a promising avenue enhance non-fouling efficacy. Microbial synthesis such nanoscale metallic structures is substantive interest this can offer an eco-friendly, cost-effective, sustainable route further development. Here we present Mucor hiemalis-derived fungal silver nanoparticles, which display significant antimicrobial properties when...
The successful development of a noninvasive blood glucose sensor that can operate reliably over sustained periods time has been much sought after but elusive goal in diabetes management. Since no well-established cure, control elevated levels is critical for avoiding severe secondary health complications multiple organs including the retina, kidney and vasculature. While fingerstick testing continues to be mainstay detection, advances electrochemical sensing-based minimally invasive...
Widespread testing and isolation of infected patients is a cornerstone viral outbreak management, as underscored during the ongoing COVID-19 pandemic. Here, we report large-area label-free platform that combines surface-enhanced Raman spectroscopy machine learning for rapid accurate detection SARS-CoV-2. Spectroscopic signatures acquired from virus samples on metal–insulator–metal nanostructures, fabricated using nanoimprint lithography transfer printing, can provide test results within 25...
We present the first demonstration of glycated albumin detection and quantification using Raman spectroscopy without addition reagents. Glycated is an important marker for monitoring long-term glycemic history diabetics, especially as its concentrations, in contrast to hemoglobin levels, are unaffected by changes erythrocyte life times. Clinically, concentrations show a strong correlation with development serious diabetes complications including nephropathy retinopathy. In this article, we...
In recent years, glycated hemoglobin (HbA1c) has been increasingly accepted as a functional metric of mean blood glucose in the treatment diabetic patients. Importantly, HbA1c provides an alternate measure total glycemic exposure due to representation throughout day, including post-prandially. this article, we propose and demonstrate potential Raman spectroscopy novel analytical method for quantitative detection HbA1c, without using external dyes or reagents. Using drop coating deposition...
Despite the intrinsic elemental analysis capability and lack of sample preparation requirements, laser-induced breakdown spectroscopy (LIBS) has not been extensively used for real-world applications, e.g., quality assurance process monitoring. Specifically, variability in sample, system, experimental parameters LIBS studies present a substantive hurdle robust classification, even when standard multivariate chemometric techniques are analysis. Considering pharmaceutical investigation as an...
Circulating biomarkers have emerged as promising non-invasive, real-time surrogates for cancer diagnosis, prognostication and monitoring of therapeutic response. Emerging data, however, suggest that single markers are inadequate in describing complex pathologic transformations. Architecting assays capable parallel measurements multiple can help achieve the desired clinical sensitivity specificity while conserving patient specimen reducing turn-around time. Here we describe a plasmon-enhanced...
The ability to detect trace analytes without necessitating solid surface attachment or complicated processing steps would facilitate the translation of sensors for monitoring environmental toxins in field. To address a critical unmet need fresh water ecology, we have developed dual-modal aptamer-based biosensor (aptasensor), featuring fluorescence and surface-enhanced Raman spectroscopy (SERS), sensitive selective detection hepatotoxin microcystin-LR (MC-LR). rational sensor design is based...
Abstract Plasmonic nanomaterials bearing targeting ligands are of great interest for surface‐enhanced Raman scattering (SERS)‐based bioimaging applications. However, the practical utility SERS‐based imaging strategies is hindered by lack a straightforward method to synthesize highly sensitive SERS‐active nanostructures with high yield and efficiency. In this work, leveraging DNA origami principles, first‐in‐class design plasmonically coupled nanoprobe targeted cancer (SPECTRA) reported. The...
For the improved delivery of cancer therapeutics and imaging agents, conjugation cell-penetrating peptides (CPPs) increases cellular uptake water solubility agents. Among various CPPs, arginine-rich have been most widely used. Combining CPPs with enzyme-responsive presents an innovative strategy to target specific intracellular enzymes in cells when combined appropriate click chemistry can enhance theranostic drug through formation self-assembled nanostructures. However, one drawback is...
We have developed a novel multimodal microscopy system that incorporates confocal Raman, reflectance, and quantitative phase (QPM) into single imaging entity.Confocal Raman provides detailed chemical information from the sample, while reflectance show morphology.Combining these intrinsic contrast modalities makes it possible to obtain morphological without exogenous staining.For validation characterization, we used this multi-modal investigate healthy diseased blood samples.We first...
We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to signal arising from development malaria-inducing parasites. By selectively imaging electric fields using quantitative phase microscopy a Fourier transform technique, we calculate maps Pf-RBCs. show that onset progression pathological states Pf-RBCs can be clearly identified by static maps. Progressive changes biophysical properties Pf-RBC...
Abstract Microcalcifications are a feature of diagnostic significance on mammogram and target for stereotactic breast needle biopsy. Here, we report development Raman spectroscopy technique to simultaneously identify microcalcification status diagnose the underlying lesion, in real-time, during core biopsy procedures. spectra were obtained ex vivo from 146 tissue sites fresh cores 33 patients, including 50 normal sites, 77 lesions with microcalcifications, 19 without using compact clinical...
Abstract Surface‐enhanced Raman spectroscopy (SERS) has evolved from an esoteric physical phenomenon to a robust and effective analytical method recently. The need of addressing both the field enhancement extinction nanoparticle suspensions, however, been underappreciated despite its substantive impact on sensing performance. A systematic experimental investigation SERS attenuation is performed in suspensions gold nanostars, which exhibit markedly different behavior relation conventional...
Abstract Reported is a new shell‐based spectroscopic platform, named mechanical trap surface‐enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on membrane single live cells. By leveraging functionalization inner surfaces MTs with plasmonic gold nanostars, conformal contact cell membrane, MTSERS permits excellent signal enhancement, reliably detects signatures, allows non‐perturbative, multiplex surface...
Despite its intrinsic advantages, translation of laser induced breakdown spectroscopy for material identification has been often impeded by the lack robustness developed classification models, due to presence spurious correlations. While a number classifiers exhibiting high discriminatory power have reported, efforts in establishing subset relevant spectral features that enable fundamental interpretation segmentation capability and avoid 'curse dimensionality' lacking. Using LIBS data...
Abstract Delay in the assessment of tumor response to radiotherapy continues pose a major challenge quality life for patients with nonresponsive tumors. Here, we exploited label-free Raman spectroscopic mapping elucidate radiation-induced biomolecular changes tumors and uncovered latent microenvironmental differences between treatment-resistant -sensitive We used isogenic radiation-resistant A549 human lung cancer cells head neck squamous cell carcinoma (HNSCC) lines (UM-SCC-47 UM-SCC-22B,...
Recent advances in animal modeling, imaging technology, and functional genomics have permitted precise molecular observations of the metastatic process. However, a comprehensive understanding premetastatic niche remains elusive, owing to limited tools that can map subtle differences mediators organ-specific microenvironments. Here, we report ability detect changes lung microenvironment, response primary breast tumors, using combination mouse models, Raman spectroscopy, multivariate analysis...