A5022095208- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Congenital Heart Disease Studies
- Aortic Disease and Treatment Approaches
- Cardiac Valve Diseases and Treatments
- 3D Printing in Biomedical Research
- Cardiac and Coronary Surgery Techniques
- Cardiac Structural Anomalies and Repair
- Mechanical Circulatory Support Devices
- Aortic aneurysm repair treatments
- Infectious Aortic and Vascular Conditions
- Additive Manufacturing and 3D Printing Technologies
- Pulmonary Hypertension Research and Treatments
- Infective Endocarditis Diagnosis and Management
- Transplantation: Methods and Outcomes
- Congenital Diaphragmatic Hernia Studies
- Anatomy and Medical Technology
- Tracheal and airway disorders
- Connective tissue disorders research
- Congenital heart defects research
- Cardiovascular Issues in Pregnancy
- Pluripotent Stem Cells Research
- Coronary Artery Anomalies
- Surgical Simulation and Training
- Vascular anomalies and interventions
University of Chicago
2018-2025
Advocate Children's Hospital
2018-2025
University of Illinois Chicago
2023-2024
University of Chicago Medical Center
2021-2023
Advocate Heart Institute
2022-2023
Johns Hopkins University
2016-2022
Johns Hopkins Hospital
2015-2022
Hershey (United States)
2022
Comer Children's Hospital
2022
Johns Hopkins Medicine
2016-2020
Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are the earliest tissue-engineered vascular grafts (TEVGs) to be used clinically. These TEVGs transform into living blood vessels in vivo, an endothelial cell (EC) lining invested by smooth muscle (SMCs); however, process which this occurs is unclear. To test if BMCs differentiate mature of neovessel, we implanted immunodeficient mouse recipient human BMC (hBMC)-seeded scaffolds. As humans, a host as venous...
Bioprinting is a technology with the prospect to change way many diseases are treated, by replacing damaged tissues live de novo created biosimilar constructs. However, after more than decade of incubation and proofs concept, field still in its infancy. The current stagnation consequence early success: first bioprinters, most those that followed, were modified versions three-dimensional printers used additive manufacturing, redesigned for layer-by-layer dispersion biomaterials. In all...
We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial (EC) were aggregated create mixed cell spheroids. Cardiac patches created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) bioprinter. analyzed with light video microscopy, immunohistochemistry, immunofluorescence, viability assays optical electrical mapping. tissue all...
Tissue engineered vascular grafts (TEVGs) have the potential to overcome issues faced by existing small diameter prosthetic providing a biodegradable scaffold where patient's own cells can engraft and form functional neotissue. However, applying classical approaches create arterial TEVGs using slow degrading materials with supraphysiological mechanical properties, typically results in limited host cell infiltration, poor remodeling, stenosis, calcification. The purpose of this study is...
The primary graft-related complication during the first clinical trial evaluating use of tissue-engineered vascular grafts (TEVGs) was stenosis. We investigated role macrophages in formation TEVG stenosis a murine model. analyzed natural history macrophage infiltration at critical time points and evaluated cell seeding on neovessel formation. To assess function infiltrating macrophages, we implanted TEVGs into mice that had been depleted using clodronate liposomes. confirm this, used...
Tissue-engineered vascular autografts (TEVAs) were made by seeding 4-6 x 10(6) of mixed cells obtained from femoral veins mongrel dogs onto tube-shaped biodegradable polymer scaffolds composed a polyglycolid acid (PGA) nonwoven fabric sheet and copolymer L-lactide caprolactone (n = 4). After 7 days, the inferior vena cavas (IVCs) same replaced with TEVAs. 3, 4, 5, 6 months, angiographies performed, sacrificed. The implanted TEVAs examined both grossly immunohistologically. showed no evidence...
In Brief Introduction: The development of a living, autologous vascular graft with the ability to grow holds great promise for advancing field pediatric cardiothoracic surgery. Objective: To evaluate growth potential tissue-engineered (TEVG) in juvenile animal model. Methods: Polyglycolic acid nonwoven mesh tubes (3-cm length, 1.3-cm id; Concordia Fibers) coated 10% copolymer solution 50:50 l-lactide and ε-caprolactone were statically seeded 1 × 106 cells/cm2 bone marrow derived mononuclear...
Congenital heart defect interventions may benefit from the fabrication of patient-specific vascular grafts because wide array anatomies present in children with cardiovascular defects. 3D printing is used to establish a platform for production custom grafts, which are biodegradable, mechanically compatible tissues, and support neotissue formation growth. As service our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed be...
We developed a tissue-engineered vascular graft composed of biodegradable scaffold seeded with autologous bone marrow-derived mononuclear cells (BMMCs) that is currently in clinical trial and analogous mouse models to study mechanisms neovessel formation. previously reported human BMMCs were rapidly lost after implantation into immunodeficient mice as host macrophages invaded the graft. As consequence, resulting was entirely cell origin. Here, we investigate source neotissue syngeneic...
The distribution of periodic patterns materials with radial or bilateral symmetry is a universal natural design principle. Among the many biological forms, tubular shapes are common motif in organisms, and they also important for bioimplants soft robots. However, simple principle strategic placement 3D printed segments swelling nonswelling to achieve widely different functionalities yet be demonstrated. Here, we report design, fabrication, characterization segmented gel tubes composed an...
We developed a tissue-engineered vascular graft (TEVG) for use in children and present results of U.S. Food Drug Administration (FDA)-approved clinical trial evaluating this patients with single-ventricle cardiac anomalies. The TEVG was used as Fontan conduit to connect the inferior vena cava pulmonary artery, but high incidence narrowing manifested within first 6 months, which treated successfully angioplasty. To elucidate mechanisms underlying early stenosis, we data-informed,...
One of the leading causes death worldwide is heart failure. Despite advances in treatment and prevention failure, number affected patients continues to increase. We have recently developed 3D-bioprinted biomaterial-free cardiac tissue that has potential improve function. This study aims evaluate vivo regenerative these patches. The patches were generated using 3D-bioprinting technology conjunction with cellular spheroids created from a coculture human-induced pluripotent stem cell-derived...